BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0042.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 436 e-124 gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 115 3e-27 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 436 bits (1121), Expect = e-124, Method: Compositional matrix adjust. Identities = 210/227 (92%), Positives = 223/227 (98%) Query: 1 MSFAIPAPAQATVPVVGGAPFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVV 60 MSFAIPAPAQATV VVGGA FPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVV Sbjct: 1 MSFAIPAPAQATVAVVGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVV 60 Query: 61 PTDSVVPYPAATADYHHEIELVVALSGGGRDIPVEKALELVYGYAVGLDMTRRDLQAQAK 120 PTDSVVPYP AT+DYHHEIELVVAL+ GGR+IPVE+ALELV+GYAVGLDMTRRD+Q+QAK Sbjct: 61 PTDSVVPYPVATSDYHHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAK 120 Query: 121 KLGRPWDMAKGFDQSAPCAPIVPVAQSGHPDKGAVWLKVNGEVRQQGDLSDLIWNVAETI 180 KLGRPWDMAKGFDQSAPCAPIVPVAQ+GHPD+GAVWLKVNGEVRQQGDL+DLIW+VAETI Sbjct: 121 KLGRPWDMAKGFDQSAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETI 180 Query: 181 SYLSGLVELFPGDLIYTGTPEGVGAVVKGDQLQGHVDGLPDINITIG 227 SYLSGLVELFPGDLI+TGTPEGVGAVVKGD+LQGHVDGLPDINITIG Sbjct: 181 SYLSGLVELFPGDLIFTGTPEGVGAVVKGDKLQGHVDGLPDINITIG 227 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 115 bits (288), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 82/223 (36%), Positives = 112/223 (50%), Gaps = 19/223 (8%) Query: 12 TVPVVGGAPF------PVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSV 65 T+PVV G P + + C+G NYA HA E + P P F K AVV + Sbjct: 53 TLPVVEGNPRIGACVGNIGKFICIGLNYADHAAE-SNLPIPAEPVVFNKWTSAVVGPNDN 111 Query: 66 VPYPAATADYHHEIELVVALSGGGRDIPVEKALELVYGYAVGLDMTRRDLQAQAKKLGRP 125 V P + E+EL V + GG I + A+ V GY V D++ R+ Q + G Sbjct: 112 VKIPRGSKKTDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIER---GGT 168 Query: 126 WDMAKGFDQSAPCAP-IVPVAQSGHPDKGAVWLKVNGEVRQQGDLSDLIWNVAETISYLS 184 WD KG D P P +V + P K +WL+V+G+ Q G+ S +I+NVA +SYLS Sbjct: 169 WDKGKGCDTFGPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLS 228 Query: 185 GLVELFPGDLIYTGTPEGVGAVVK--------GDQLQGHVDGL 219 + L PGD+I TGTP GVG VK G ++ +DGL Sbjct: 229 RFMSLQPGDVISTGTPPGVGMGVKPEAVYLRAGQTIRLGIDGL 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 880,439 Number of extensions: 41924 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 2 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)