BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0173.p (322 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 597 e-172 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 382 e-107 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 68 1e-12 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 57 2e-09 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 597 bits (1539), Expect = e-172, Method: Compositional matrix adjust. Identities = 315/343 (91%), Positives = 318/343 (92%), Gaps = 22/343 (6%) Query: 1 VQLLERIERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQN 60 VQLLER+ERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQN Sbjct: 145 VQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQN 204 Query: 61 GRREMGSSGGGGRYDYGYFSDTLLEKYASEAVATL---------------------WXPG 99 GRREMGSSGGGGRYDYGYFSD LLE+YASEAVAT W PG Sbjct: 205 GRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRPAPAGPMTVVLGPGW-PG 263 Query: 100 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 159 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT Sbjct: 264 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 323 Query: 160 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDKDPGEI 219 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGD+DP EI Sbjct: 324 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDRDPAEI 383 Query: 220 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 279 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV Sbjct: 384 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 443 Query: 280 SMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 322 SMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA Sbjct: 444 SMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 486 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 382 bits (982), Expect = e-107, Method: Compositional matrix adjust. Identities = 195/343 (56%), Positives = 243/343 (70%), Gaps = 22/343 (6%) Query: 1 VQLLERIERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQN 60 + LLE ++R RA+ P V +VMASLS D+V +AR DG D+RPLVR+ V V +N Sbjct: 160 IALLESVDRACRARSPLVVEVMASLSISLDLVFLARNDGYCGGDVRPLVRLDVAVQVVRN 219 Query: 61 GRREMGSSGGGGRYDYGYFSDTLLE----------------KYASEAVATL-----WXPG 99 GRRE S GGR F +E + A T+ W G Sbjct: 220 GRREGASRTLGGRVGLATFDHAAIESVAALAVDAALIKLEARAAPAGTMTVVIGPGWN-G 278 Query: 100 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 159 ++LHEA+GHGLEGDFNR+GSS F+GRIG+RVAA GVT+VDDGT+ RGSLNIDDEG P Sbjct: 279 VMLHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPG 338 Query: 160 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDKDPGEI 219 QCTTLIEDGIL Y+QD++NARLM + TGN RRES+ LPMPRMTNTYM G DP EI Sbjct: 339 QCTTLIEDGILVSYMQDSLNARLMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEI 398 Query: 220 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 279 +ASVK G+Y + GGQVDI++G+F+F+ASEA++IE+G++ PVKGAT++GNGP L V Sbjct: 399 IASVKQGIYIAHLEGGQVDISSGRFMFAASEAFLIENGRLGAPVKGATVLGNGPQSLKNV 458 Query: 280 SMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 322 +IGND+ +++ GVCGK GQSVPVGVGQPTLR+DG+TVGGTA Sbjct: 459 KLIGNDLEINTASGVCGKAGQSVPVGVGQPTLRIDGMTVGGTA 501 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 71/309 (22%), Positives = 129/309 (41%), Gaps = 38/309 (12%) Query: 1 VQLLERIERIARAKDPRVKQVM-ASLSGEYDVVLVARADGVLAADIRPLVRVSVTV---- 55 V L +R E A DPR+ A + ++ + A + G + P R +++V Sbjct: 138 VALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMGG--YPFSRHTISVAPIA 195 Query: 56 -----------IAEQNGRREMGSSGGGGRYDYGYFSDTLLEKYASEAVATLWXPGILLHE 104 + + +++ GRY ++ L + + ++T P +L Sbjct: 196 GKGASMQRDDWYSSKRDPKKLAQPEAIGRYA----AERALARLNARKLSTRKCP-VLFEA 250 Query: 105 AIGHGLEGDFNR--------KGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEG 156 + GL G F + + S+ +G++V + + +++D + GS D+EG Sbjct: 251 PLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGGVGSAPFDEEG 310 Query: 157 NPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDKDP 216 T ++ +G+++GY T +AR + M TGN+ L T G Sbjct: 311 VRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSELTTRADTFKG---- 366 Query: 217 GEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVL 276 +L + GL G V+ G + AS Y +E+G I YPV+ T+ GN D+L Sbjct: 367 --MLKKMGTGLLVTELMGQGVNYVTGDYSRGAS-GYWVENGVIQYPVEEITIAGNMKDML 423 Query: 277 NRVSMIGND 285 ++ +GND Sbjct: 424 RQIVAVGND 432 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 73/304 (24%), Positives = 120/304 (39%), Gaps = 28/304 (9%) Query: 1 VQLLERIERIARAKDPRVKQV-MASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQ 59 V L+ RIER A A V Q+ A L ++A ++G L + +S T +A + Sbjct: 141 VALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSLSCTAVAIR 200 Query: 60 NGRREMGSSGGGGRYDYGYFSDT----------LLEKYASEAVATLWXPGILLHEAIGHG 109 +G +E+ S R D L + + VAT P +L Sbjct: 201 DGAKEI-ESWWDARRDPAQLPSPESLGACAGRRALARLGARQVATQTCP-VLFEPQAAAS 258 Query: 110 LEGDFNRK--------GSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPTQC 161 L +F + SS +G+ V A + + DD + GS + D G + Sbjct: 259 LLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAALRP 318 Query: 162 TTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDKDPGEILA 221 TLI G L+GY AR + + + + P M ++ D ++ Sbjct: 319 RTLIAGGRLQGYYLGAYGARRLGLPLAEHGAG------PSNVMVSSTTSAASGDLEAMIR 372 Query: 222 SVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRVSM 281 + GL G V++ G F + S + +E+G+I YPV G T+ N ++ + Sbjct: 373 RMGTGLVISGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGIVA 431 Query: 282 IGND 285 +GND Sbjct: 432 VGND 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 983,574 Number of extensions: 41776 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 116 Number of HSP's successfully gapped: 4 Length of query: 322 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 237 Effective length of database: 1,217,395 Effective search space: 288522615 Effective search space used: 288522615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)