BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0158.p (577 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 677 0.0 gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 331 9e-92 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 126 5e-30 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 101 1e-22 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 100 5e-22 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 677 bits (1748), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/445 (80%), Positives = 397/445 (89%), Gaps = 3/445 (0%) Query: 1 MTATHALQQAAPEAAPTTIAATLAAYREGRSTPGKVVAELLARIESAGRDEVWISRVPSK 60 M H QQ A AP TIA+TLAAYR G STPGK VA LLA IE A R+EVWISRVP+K Sbjct: 1 MKPAHIPQQVA---APVTIASTLAAYRNGSSTPGKTVASLLALIEQADREEVWISRVPAK 57 Query: 61 QLKQQAAALDLMLQIKGDAVLESMPLFGIPFAVKDNIDVAEMSTTGACPEFGRLASESAT 120 QLKQQAAALDLM QIKG+ VLESMPLFGIPFAVKDNIDVA M TT ACPEF R+A SAT Sbjct: 58 QLKQQAAALDLMWQIKGETVLESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSAT 117 Query: 121 VVQRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIKPEYVSGGSSSGSAVAVALGQ 180 VVQRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAI P+YVSGGSSSGSAVAVALGQ Sbjct: 118 VVQRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQ 177 Query: 181 VMFSLGTDTAGSGRVPAAFNHLVGLKPTKGLISARGLVPACRTLDCISIFAHDVADAWRV 240 VMF+LGTDTAGSGR+PAAFNHLVGLKP++GLISA+GLVPACRTLDCISIFAHDVADAW V Sbjct: 178 VMFALGTDTAGSGRIPAAFNHLVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHV 237 Query: 241 LHSASGFDAEDGYSRRPTMLGVKRRGYRIAIPEQCEFFGDSVAQEAWERSLAQIAALPGV 300 LHSA+G+DA D Y+R+ MLG+KRRGYRIAIP EF+GD AQ+AW+R+LA+IA LPGV Sbjct: 238 LHSAAGYDAADAYARKLPMLGLKRRGYRIAIPAATEFYGDLAAQDAWDRTLARIAELPGV 297 Query: 301 SLAKIPFAPFSEAAQLLYQGPWVAERRAALGDFFLENPEAIHPVVRQITAQADDYDAVDS 360 +LA+IPFAPF++AA+LLYQGPWVAERRAALGDFF++ P+A+HPVVR+ITAQA DYDAV + Sbjct: 298 TLARIPFAPFADAARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAA 357 Query: 361 FNAQYRLADLRRQAENLLEGSDLMLVPTTTTFPTIADVEADPVVRNAQLGHYTNFVNLMD 420 F QYRLA+LRRQAE LLEG+DLMLVPTTT PTIA+V+ADPV RN+QLG+YTNFVNLMD Sbjct: 358 FQGQYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMD 417 Query: 421 MSALAIPAVWRRDGLPAGVTLIGPA 445 MSALAIP +WR DGLPAGVTLIGPA Sbjct: 418 MSALAIPGLWRNDGLPAGVTLIGPA 442 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 331 bits (849), Expect = 9e-92, Method: Compositional matrix adjust. Identities = 201/434 (46%), Positives = 272/434 (62%), Gaps = 16/434 (3%) Query: 19 IAATLAAYREGRSTPGKVVAELLARIESAGRDEVWISRVPSKQLKQQAAALDLMLQIKGD 78 I A AAYR G P V+ L R +A + + R+ L Q A + LQ Sbjct: 9 IDALTAAYRNGSLDPRTVMQRL--RESAAALNPTY--RLYIHLLSQ--AETEPYLQALEQ 62 Query: 79 AVLESMPLFGIPFAVKDNIDVAEMSTTGACPEFGRLASESATVVQRLQAAGALLVGKTNL 138 S+PL+G+PF++KDNID+A + TT ACP F + ESAT+V +L A GA+ VGK NL Sbjct: 63 QSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLVAQLIALGAIPVGKCNL 122 Query: 139 DQFATGLVGVRSPYGAVRNAIKPEYVSGGSSSGSAVAVALGQVMFSLGTDTAGSGRVPAA 198 DQFATGL G RSPYGA RN++ P+Y SGGSSSGS++AVALG F+LGTDTAGSGRVPAA Sbjct: 123 DQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAA 182 Query: 199 FNHLVGLKPTKGLISARGLVPACRTLDCISIFAHDVADAWRVLH--SASGF-DAEDGYSR 255 N+LVGLK TKGL+S G+VPACRTLDC++ F A+A +L + SG D D YSR Sbjct: 183 LNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEYSR 242 Query: 256 ------RPTMLGVKRRGYRIAIPEQCEFFGDSVAQEAWERSLAQIAALPGVSLAKIPFAP 309 + +G R +P Q EF G S + + +LAQ+ A+ GV++ I F+P Sbjct: 243 ANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQMEAIGGVAV-PIDFSP 301 Query: 310 FSEAAQLLYQGPWVAERRAALGDFFLENPEAIHPVVRQITAQADDYDAVDSFNAQYRLAD 369 F AA+LLY+GPWVAER + + E P+ I PV+R + +A Y AVD+F AQYRL Sbjct: 302 FLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQQ 361 Query: 370 LRRQAENLLEGSDLMLVPTTTTFPTIADVEADPVVRNAQLGHYTNFVNLMDMSALAIPAV 429 L+ + + +L D ++ P T+ ++ PV RN+ LG YTNF+N++D +A+A P Sbjct: 362 LKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPMN 421 Query: 430 WRRDGLPAGVTLIG 443 + ++GLP G+T+ G Sbjct: 422 FMKNGLPWGITVFG 435 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 129/426 (30%), Positives = 196/426 (46%), Gaps = 27/426 (6%) Query: 16 PTTIAATLAAYREGRSTPGKVVAELLARI-ESAGRDEVWISRVPSKQLKQQAAALDLMLQ 74 P+T++ A G +T ++ + LARI + AG +RV ++Q + A A D + Sbjct: 2 PSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLRA 61 Query: 75 IKGDAVLESMPLFGIPFAVKDNIDVA-EMSTTGACPEFGR-LASESATVVQRLQAAGALL 132 + L PL G+P +VKD DVA E + G+ G+ +AS A VVQ L AGA+L Sbjct: 62 AR----LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAIL 117 Query: 133 VGKTNLDQFATGLVGVRSPYGAVRNA----IKPEYVSGGSSSGSAVAVALGQVMFSLGTD 188 +GKTN+ +FA +G+ YG +NA I+ + GGSSSG+A++V G ++G+D Sbjct: 118 IGKTNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSD 177 Query: 189 TAGSGRVPAAFNHLVGLKPTKGLISARGLVPACRTLDCISIFAHDVADAWRVLHSASGFD 248 T GS R+P+A N L G KPT +S +G++P LD I A V + D Sbjct: 178 TGGSVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSV-------QCCAIMD 230 Query: 249 AEDGYSRRPTMLGVKRRGYRIAIPEQCEFFG--DSVAQEAWERSLAQIAALPGVSLAKIP 306 A +L RG R+ P G D+V Q AA + A++P Sbjct: 231 AVLAGEDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVP 290 Query: 307 FAPFSEAAQLLYQGPWV-AERRAALGDFFLENPEAIHPVVRQITAQADDYDAVDSFNAQY 365 F+ A + +G + AE A EN V + D A D + + Sbjct: 291 --AFNALAAINAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLH 348 Query: 366 RLADLRRQAENLLEGSDLMLVPTT-TTFPTIADVEADPVVR---NAQLGHYTNFVNLMDM 421 Q E L G D +++PT P IA+++A N ++ +N +D Sbjct: 349 ARPRWIAQVEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDG 408 Query: 422 SALAIP 427 AL++P Sbjct: 409 CALSLP 414 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 132/464 (28%), Positives = 195/464 (42%), Gaps = 66/464 (14%) Query: 19 IAATLAAYREG----RSTPGKVVAELLARIESAGRDEVWISRVPSKQLKQQAAALDLMLQ 74 + TL R+G R + + V L+A E+ ++ R P +QL+ QA LD + Sbjct: 4 VDMTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDP-EQLRSQARMLD---E 59 Query: 75 IKGDAVLESMPLFGIPFAVKDNIDVAEM-STTGACPEFGRLASESATVVQRLQAAGALLV 133 G PL GIP A+KDNIDVA + ST G + ATVV+ L AGAL Sbjct: 60 QGGHG-----PLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHA 114 Query: 134 GKTNLDQFATGLVGVRSPYGAVRNAIKPEYVSGGSSSGSAVAVALGQVMFSLGTDTAGSG 193 GK N+ + A G+ +G RN E ++GGSS G +A ++GTDT GS Sbjct: 115 GKANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSV 174 Query: 194 RVPAAFNHLVGLKPTKGLISARGLVPACRTLDCISIFAHDVADAWRVLHSASGF-DAEDG 252 R+PAA +VGL+PT S G+VP T D A VAD + +G D+ Sbjct: 175 RIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPL 234 Query: 253 YSRRPTM--LGVKRRGYRIAIPEQCEFFGDSVAQEAWERSLAQIAALPGVSLAKI---PF 307 + P+ LGV R + E+ E ++ + E GV L + Sbjct: 235 LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLRE---------AGVELVDVDLPTL 285 Query: 308 APFSEAAQLLYQGPWVAERRAALGDFFLENPEAIH-----PVVRQITAQADDYDAVDSFN 362 AP S AA L +F ++ P + V++++ A+ D Sbjct: 286 APLSHAAGFAI----------VLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQ 335 Query: 363 AQ---------YRLA-DLRRQAENLL------EGSDLMLVPTTTTFPTIADVEADPVVRN 406 A YRLA + RR + L +G D ++ PTT + +R Sbjct: 336 ATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRG 395 Query: 407 AQLGHYTNFVNLMD------MSALAIPAVWRRDGLPAGVTLIGP 444 ++ + F D + +++IP GLP G+ L GP Sbjct: 396 ERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGP 439 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 99.8 bits (247), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 115/399 (28%), Positives = 180/399 (45%), Gaps = 54/399 (13%) Query: 85 PLFGIPFAVKDNIDVAEMSTTGACPEFGRLASES-ATVVQRLQAAGALLVGKTNLDQFAT 143 PL GIP VKDN D +M TTG L +++ A V+RL+ AGA+++GKT + + A Sbjct: 118 PLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHELAA 177 Query: 144 GLVGVRSPYGAVRNAIKPEYVSGGSSSGSAVAVALGQVMFSLGTDTAGSGRVPAAFNHLV 203 G+ V S G RN P GGSS G+ AVA +G+DT GS R+PAA +L Sbjct: 178 GVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLF 237 Query: 204 GLKPTKGLISARGLVPACRTLDCISIFAHDVADAWRVLHSASGFDAEDG----------Y 253 GL+ T+GL S G++P T D + A V D +L + G D +D Sbjct: 238 GLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHIPK 297 Query: 254 SRRPTMLGVKRRGYRIAI--------PEQCEFFGDSVAQEAWERSLAQIAALPGVSLAKI 305 S R + +G RI + PE E + +A ++ Q A + V++ ++ Sbjct: 298 SYRDGLRADSLQGARIGVLRALFGAAPEDAEV--SAAINKALQQLKDQGAIVTDVTIPEL 355 Query: 306 PFAPFSEAAQLLYQGPWVAERRAALGDFFLENPEAIHPVVRQITAQADDYDAVDSFNAQY 365 S ++ + Y E + LG + +P A + +I A+ +++ +++ Sbjct: 356 D-GLLSGSSIIPY------EFKYDLGAYLQSHPGAPVGSLGEILARGMEHELLEAGLRLR 408 Query: 366 RLADLRRQAEN------LLEGSDLMLVPT---------TTTFPTI---ADVEADPV--VR 405 DL+ + +L+ S L + T T +PTI A + +P Sbjct: 409 NSVDLQNPKDKEELEKVMLKRSALKSLMTDVMQKNHLDTLVYPTIQRKAALIGEPQGGAM 468 Query: 406 NAQLGHYTNFVNLMDMSALAIPAVWRRDGLPAGVTLIGP 444 N QL T + ALA+P + DGLP + L+ P Sbjct: 469 NCQLSATTG------LPALALPVGFTEDGLPVSLELLAP 501 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,639,425 Number of extensions: 67714 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 11 Length of query: 577 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 487 Effective length of database: 1,193,720 Effective search space: 581341640 Effective search space used: 581341640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)