BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0148.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310740|ref|YP_003774832.1| isochorismatase family protein ... 434 e-123 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 209 2e-55 gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoa... 65 3e-12 gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoa... 55 5e-09 >gi|300310740|ref|YP_003774832.1| isochorismatase family protein [Herbaspirillum seropedicae SmR1] Length = 227 Score = 434 bits (1116), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/235 (91%), Positives = 217/235 (92%), Gaps = 8/235 (3%) Query: 1 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLAXR 60 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLA Sbjct: 1 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLAL- 59 Query: 61 MHGGTTTARQAQMLVVHTRESHLPDLSDCPRAKRLRGNPTLGIGDVGPMGRILVRGEPGN 120 ARQ QMLVVHTRESHLPDLSDCPR KRLRGNPTLGIGDVGPMGRILVRGEPGN Sbjct: 60 -------ARQTQMLVVHTRESHLPDLSDCPRTKRLRGNPTLGIGDVGPMGRILVRGEPGN 112 Query: 121 QILPQLAPLDGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREAND 180 QILPQL P+ GEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREAND Sbjct: 113 QILPQLTPMAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREAND 172 Query: 181 RGYECLVVEDACASYFPEFHRATLEMLTAQGGIVGWRAPLAQLHSAVAAYTGETP 235 RGYECLVVEDACASYFP FHRATL+MLTAQGGIVGWRAPLAQL SAV AY GE P Sbjct: 173 RGYECLVVEDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTAYAGEHP 227 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 209 bits (532), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 111/235 (47%), Positives = 148/235 (62%), Gaps = 14/235 (5%) Query: 1 MIRIDAFPYPYQ--FHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLA 58 I+ + +P+PY P +TAL+VIDMQ DF GG+ +G D+ A + + +LA Sbjct: 5 FIQAEPYPWPYDGALTPANTALIVIDMQTDFCGIGGYVDKMGYDLSLTRAPIEPIRKVLA 64 Query: 59 XRMHGGTTTARQAQMLVVHTRESHLPDLSDCPRAKRLR----GNPTLGIGDVGPMGRILV 114 GG T ++HTRE H PDLSD P KR R G +GIGD GP GRILV Sbjct: 65 AMRAGGYT--------IIHTREGHRPDLSDLPANKRWRSRQIGANGVGIGDAGPCGRILV 116 Query: 115 RGEPGNQILPQLAPLDGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTS 174 RGEPG +I+P+LAP+ GEI+IDKPGKG+F ATDL L RGI ++++ G+TT+VCV T+ Sbjct: 117 RGEPGWEIIPELAPIAGEIIIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVHTT 176 Query: 175 MREANDRGYECLVVEDACASYFPEFHRATLEMLTAQGGIVGWRAPLAQLHSAVAA 229 MREANDRG+EC+++ D C + H A L M+ QGG+ G A A L + + A Sbjct: 177 MREANDRGFECVMLSDCCGATDYNNHLAALSMIKMQGGVFGAVADSAALIAVIGA 231 >gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 65.5 bits (158), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 36/87 (41%), Positives = 48/87 (55%) Query: 118 PGNQILPQLAPLDGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMRE 177 PG+ I+PQLAP +GE+V+ K AF+ T L L + G+ LLVAG T CV+ S+ + Sbjct: 98 PGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVD 157 Query: 178 ANDRGYECLVVEDACASYFPEFHRATL 204 A G+ LVV D H A L Sbjct: 158 AMSWGFRPLVVSDCVGDRALGPHEANL 184 >gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 176 Score = 55.1 bits (131), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 29/79 (36%), Positives = 47/79 (59%) Query: 113 LVRGEPGNQILPQLAPLDGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQ 172 L RG G Q++P L ++ + K G AF+ T+L A L+ R I+ L++ G+ TE CV+ Sbjct: 56 LQRGSEGWQLMPGLHQQPEDLHLPKRGSDAFHHTELQAMLKMRHISALVICGMQTEYCVE 115 Query: 173 TSMREANDRGYECLVVEDA 191 +++R A GY ++ DA Sbjct: 116 STVRRALALGYPVMLAADA 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 820,242 Number of extensions: 35974 Number of successful extensions: 94 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 5 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)