BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0143.p (809 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 1539 0.0 gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 139 6e-34 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 1539 bits (3984), Expect = 0.0, Method: Compositional matrix adjust. Identities = 739/809 (91%), Positives = 769/809 (95%), Gaps = 15/809 (1%) Query: 1 MNFSNEADELRALGTILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPF 60 MNFSNEADELRALG ILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPF Sbjct: 171 MNFSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPF 230 Query: 61 AEHDKIATAFGLADLPTRNGYIVIWTTTPWTIPSNQALNVHPEFEYALVETSRNGEATLL 120 AEHDK+ATAFGL DLPTR+GYIVIWTTTPWTIP+NQALNVHPEFEYALVETSRNGEATLL Sbjct: 231 AEHDKVATAFGLTDLPTRHGYIVIWTTTPWTIPANQALNVHPEFEYALVETSRNGEATLL 290 Query: 121 ILAKDLVESCLQRFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPIYLGEYVTAD 180 ILAKDLV SCL+RFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSP++LGEYVTAD Sbjct: 291 ILAKDLVASCLERFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTAD 350 Query: 181 SGTGIVHSAPAYGIEDFISCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPIC 240 SGTGIVHS+PAYGIEDF+SCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPIC Sbjct: 351 SGTGIVHSSPAYGIEDFVSCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPIC 410 Query: 241 GKLEEVDSLFKLVMFDHSYMHCWRHKTPIVYRATSQWFASMDNTPKDGGPSLRQTALQGI 300 KLEE+ SLFKL+MF HSYMHCWRHK+PI+YRATSQWFASMDNTPKDGGPSLRQTALQGI Sbjct: 411 DKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGI 470 Query: 301 EDTAFFPGWGKARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELIEQVAQ 360 EDTAFFPGWGKARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPEL+EQ+AQ Sbjct: 471 EDTAFFPGWGKARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELLEQIAQ 530 Query: 361 RIEKSGIEAWQALDPKELLGDEAEHYLKNRDTLDVWFDSGTTHQTVLRGSHAQQSQFPAD 420 RIE+ GIEAWQALDPKELLGDEA+ YLKN+DTLDVWFDSG+THQTVLRGSH Q+ QFPAD Sbjct: 531 RIEQGGIEAWQALDPKELLGDEADQYLKNKDTLDVWFDSGSTHQTVLRGSHRQELQFPAD 590 Query: 421 LYLEGSDQHRGWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGKKMSKSKGNVVAPQKVS 480 LYLEGSDQHRGWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEG+KMSKS N V PQKV+ Sbjct: 591 LYLEGSDQHRGWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVA 650 Query: 481 DSLGAEILRLWVASTDYSGELSISDEILKRVVEAYRRIRNTLRFLLANTSDFDPAQHAVP 540 DSLGAEILRLWVAS+DYSGE+SIS+EILKRV EAYRRIRNTLRFLLANTSDFDPAQHAVP Sbjct: 651 DSLGAEILRLWVASSDYSGEISISEEILKRVTEAYRRIRNTLRFLLANTSDFDPAQHAVP 710 Query: 541 VAEMLEIDRYAIANMARLQAEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYT 600 +AE+LEIDRYAIANMARLQ EVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYT Sbjct: 711 MAELLEIDRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYT 770 Query: 601 SGVESAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWRFFAGKDAFEASGETIFSQVYY 660 SGV+SAARRSAQTAVWHITQSLLRLMAPILSFTAEEAW FFAGK+AFEASGETIFSQVYY Sbjct: 771 SGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWSFFAGKEAFEASGETIFSQVYY 830 Query: 661 ALPEVADAQALLDKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGSGAWXGGG 720 ALPEVADAQ LL KFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAG Sbjct: 831 ALPEVADAQGLLAKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGE------- 883 Query: 721 GGGGGGGPPAALLESLGDDLKFVLITSAARVTPVATVEEEAVVVTPSAHQKCERCWHYRA 780 AALLESLGDDLKFVLITSAARVT VA+VEEEAVVVTPSAHQKCERCWHYRA Sbjct: 884 --------KAALLESLGDDLKFVLITSAARVTRVASVEEEAVVVTPSAHQKCERCWHYRA 935 Query: 781 DVGSHADHPGLCGRCYSNLFGQGEKRLFA 809 DVGSHA+H GLCGRC SNLFGQGEKR FA Sbjct: 936 DVGSHAEHAGLCGRCVSNLFGQGEKRQFA 964 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 139 bits (351), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 173/703 (24%), Positives = 281/703 (39%), Gaps = 148/703 (21%) Query: 16 ILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKIATAFGLADL 75 + E+G +YRG + VNW G+A+++ EV +++ + +P A+ Sbjct: 196 LYEQGLIYRGKRLVNWDPVLGTAVSDLEVVSEEEDGSMWHIKYPLADG------------ 243 Query: 76 PTRNGYIVIWTTTPWTIPSNQALNVHPEFE-YALVETSRNGEATLLILAKDLVESCLQRF 134 +G+IV+ TT P T+ + A+ V P E YA Sbjct: 244 ---SGHIVVATTRPETMLGDVAVAVDPTDERYA--------------------------- 273 Query: 135 GLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPIYLGEYVTADSGTGIVHSAPAYGI 194 L GK+I KL L+ R PI EYV + GTG V PA+ Sbjct: 274 ALVGKMI------KLPLV-------------GREIPIIKDEYVDKEFGTGCVKITPAHDF 314 Query: 195 EDFISCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICGKLEEVDSLFKLVM 254 D+ H ++ + A + GL +EA K I L+ + L ++ Sbjct: 315 NDYQVGARHNLEMISVFTLKATINENAP--EKYQGLDRFEARKQIVADLDALGLLEQVK- 371 Query: 255 FDHSYMHCWRHKTPIVYRA--TSQWFASMDNTPKDG----GPSLRQTALQGIE--DTAFF 306 H M +T +V T QWF +M +G G S+ +TAL+ + + F Sbjct: 372 -PHKLMVPRGDRTGVVIEPMLTDQWFVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFV 430 Query: 307 PGWGKARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELIEQVAQRIEKSG 366 P + + N DW +SRQ WG + + ++ G RT E E AQ +G Sbjct: 431 PENWTNTYNQWLNNIQDWCISRQLWWGHQIPAWYDEDGGIYVARTEE--EARAQ----AG 484 Query: 367 IEAWQALDPKELLGDEAEHYLKNRDTLDVWFDSGTTHQTVL---RGSHAQQSQFPADLYL 423 A + ++ D LD WF S + L + + P+ + + Sbjct: 485 GRAVK----------------RDEDVLDTWFSSALVPFSTLGWPEETPDYKLFLPSSVLV 528 Query: 424 EGSDQHRGWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGKKMSKSKGNVVAPQKVSDSL 483 G D W ++ ++ G+ P+ + HG V D G+KMSKSKGN + P + D + Sbjct: 529 TGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGI 588 Query: 484 GAEILRL----------WVASTDYSGELSISDEI-----------------LKRVV---- 512 G + L AS + + +D I L R + Sbjct: 589 GVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFASLATLGRNINFDL 648 Query: 513 ---EAYRRIRNTL----RFLLANTS----DFDPAQHAVPVAEMLEI---DRYAIANMARL 558 E YR N L RF+L NT FD + V E L+ DR+ ++ + R Sbjct: 649 NRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFSKADRWIVSLLQRT 708 Query: 559 QAEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVESAARRSAQTAVWHI 618 +AEV F Y F + + + ++ +YL++ K ++ SG E A +R+ + + + Sbjct: 709 EAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQI-QSGNE-AQQRATRRTLLRV 766 Query: 619 TQSLLRLMAPILSFTAEEAWRFFA--GKDAFEASGETIFSQVY 659 +S+LRL P+L F E+ W+ A + +G++I Q Y Sbjct: 767 LESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAY 809 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,431,456 Number of extensions: 100892 Number of successful extensions: 288 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 3 Length of query: 809 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 716 Effective length of database: 1,179,515 Effective search space: 844532740 Effective search space used: 844532740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)