BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0135.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herba... 493 e-140 gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbasp... 65 9e-12 >gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 418 Score = 493 bits (1268), Expect = e-140, Method: Compositional matrix adjust. Identities = 235/254 (92%), Positives = 247/254 (97%) Query: 1 MSYQHIQVPAEGRKITANADDTLNVPNNPIIPFIMGDGVGVDVTPVMLKVVDAAVEKAYG 60 MSYQHIQVPAEGRKITANADDTLNVPNNPIIP+I+GDGVGVDVTPVMLKVV+AAVEKAYG Sbjct: 1 MSYQHIQVPAEGRKITANADDTLNVPNNPIIPYIVGDGVGVDVTPVMLKVVNAAVEKAYG 60 Query: 61 GARKIHWMEIYAGEKSTKLYGADTWLPEETLDVLKEYLVSIKGPLSTPVGGGIRSLNVTM 120 GARKIHWMEIYAGEK+T+LYG D WLPEETL VLK+YLV+IKGPLSTPVGGGIRSLNV M Sbjct: 61 GARKIHWMEIYAGEKATRLYGPDVWLPEETLAVLKKYLVAIKGPLSTPVGGGIRSLNVAM 120 Query: 121 RQQLDLYVCLRPVRYFKGVPSPLKEPEKTDMVIFRENSEDIYAGIEWQAGTPEVKKLIEL 180 RQQLDLYVCLRPVRYFKGVPSPL+EPEKTDMVIFRENSEDIYAGIEW AGTPEV KLI+L Sbjct: 121 RQQLDLYVCLRPVRYFKGVPSPLREPEKTDMVIFRENSEDIYAGIEWAAGTPEVNKLIDL 180 Query: 181 LTREMGVKKLRFPETSALGIKPVSREGTERLVRKAIQYAIDHDKPSVTLVHKGNIMKYTE 240 LTREMGVKKLRFPE+SALGIKPVSREGTERLVR+AIQYAIDHDKPSVTLVHKGNIMK+TE Sbjct: 181 LTREMGVKKLRFPESSALGIKPVSREGTERLVRQAIQYAIDHDKPSVTLVHKGNIMKFTE 240 Query: 241 GAFRDWGYALAGRE 254 GAFRDWGYALA RE Sbjct: 241 GAFRDWGYALAARE 254 >gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 65.1 bits (157), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 62/214 (28%), Positives = 91/214 (42%), Gaps = 27/214 (12%) Query: 31 IPFIMGDGVGVDVTPVMLKVVDAAVEKAYGGARKIH--WMEI-YAGEKSTKLYGADTWLP 87 I I GDG+G +V P L+ V AA +K H W Y E + D W Sbjct: 7 IAVIAGDGIGQEVMPEGLRAVQAASKKFGIDIEFTHFDWAHCNYYAEHGKMM--PDDWF- 63 Query: 88 EETLDVLKEYLVSIKGPLSTPVGGGIRSLNVTMRQQLDLYVCLRPVRYFKGVPSPL--KE 145 E+ Y ++ P + P + + R+ DLYV LRPVR GVP PL ++ Sbjct: 64 EQLKGFEAIYFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLANRK 123 Query: 146 PEKTDMVIFRENSEDIYAGIEWQAGTPEVKKLIELLTREMGVKKLRFPETSALGIKPVSR 205 P D + REN+E Y+ I ++ E RE +++ F SR Sbjct: 124 PGDIDFYVVRENTEGEYSSIG--------GRMYEGTERETVIQESVF-----------SR 164 Query: 206 EGTERLVRKAIQYAIDHDKPSVTLVHKGNIMKYT 239 +GT+R+++ A A K +T K N + T Sbjct: 165 KGTDRILKFAFDLAQSRPKKHLTSATKSNGIAIT 198 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,283,772 Number of extensions: 54408 Number of successful extensions: 99 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 2 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)