BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0106.p (461 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310704|ref|YP_003774796.1| major facilitator superfamily p... 471 e-134 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 98 1e-21 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 76 5e-15 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 65 2e-11 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 59 8e-10 >gi|300310704|ref|YP_003774796.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 485 Score = 471 bits (1212), Expect = e-134, Method: Compositional matrix adjust. Identities = 330/455 (72%), Positives = 369/455 (81%), Gaps = 5/455 (1%) Query: 8 WRAVALTGGVALYAINVHIVTTILPSVVRDIGGLAYYAWNVTLFVAASIVGSALTGKLMG 67 W AVALTGGVA++AINVHIVTTILPSVVRDIGGL YYAWNVTLFV SI+GS LTGKL+ Sbjct: 34 WCAVALTGGVAMHAINVHIVTTILPSVVRDIGGLDYYAWNVTLFVVTSIIGSTLTGKLLA 93 Query: 68 AMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLAALAYALIPLVFEPR 127 +GARA YL+ALA+FG+GAL+C AGSMGWMLAGRAVQG+GGGLLA+L YALIPLVFE R Sbjct: 94 RLGARAAYLLALAVFGAGALVCAGAGSMGWMLAGRAVQGVGGGLLASLGYALIPLVFEQR 153 Query: 128 MWPRAIALVSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAAQAWLVWRQLGPATV 187 +W RAIALVSGMWGVSTLLGPAVGGLFAA GHWR+AFFALLPLL Q+ LV RQLGP V Sbjct: 154 LWSRAIALVSGMWGVSTLLGPAVGGLFAAGGHWRLAFFALLPLLLLQSVLVVRQLGPGRV 213 Query: 188 RSASGPAQAVPQFLLGRIALLASSVLLVALAAQADDDWLASACVLAGVLLAVWMARIDLR 247 R+ S QA+P LGRI LLA SVLLVALAAQ W A ACVL G L W+ARIDLR Sbjct: 214 RAQSDAVQAMP---LGRIFLLALSVLLVALAAQTGQAWSALACVLGGAGLGAWVARIDLR 270 Query: 248 HRANLLPHAAYRPRTVMGGVMACIALLVMGSCVEIFLPYFLQGLHGYDPLGAGYATAAMA 307 HR +LLP YR TVMG V ACI+LLVM SCVEIF+PYFLQ LHGY PL AGYA AAMA Sbjct: 271 HRISLLPRDGYRLSTVMGCVFACISLLVMASCVEIFVPYFLQSLHGYAPLAAGYAAAAMA 330 Query: 308 AGWSLGSLGGSGLSGRAALRMIRLGPCMMTLGMALLAFLMPAQWLPAGALADGLIVLALG 367 GWS+GSL GSG S +AA RMIR+GP +M LG+ LA L+PA L AG +AD LIVLAL Sbjct: 331 GGWSVGSLVGSGRSAQAAARMIRMGPWLMLLGLLALAALLPASLLAAGVVADVLIVLALA 390 Query: 368 VTGLGIGMGWPHLVARALHAAQPGEENLTSSAITTVQLFAMALGAALAGLVANQAGLG-A 426 GLGIG+GWPHLVARA+ AA+PGEENLTSSAITTVQL+AMA+GAALAGL+ANQAGLG Sbjct: 391 AAGLGIGVGWPHLVARAMQAARPGEENLTSSAITTVQLYAMAMGAALAGLIANQAGLGMP 450 Query: 427 GGAEATARAAWVLLALFTLAPALAIFVARSV-TRD 460 GG + +AA VL A+F +APALAI + R + RD Sbjct: 451 GGVDGARQAAGVLFAVFAVAPALAIMLTRRLAARD 485 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 118/440 (26%), Positives = 199/440 (45%), Gaps = 38/440 (8%) Query: 17 VALYAINVHIVTTILPSVVRDIGGLAYYAWNVTLFVAASIVGSALTGKLMGAMGARATYL 76 + L A++ IV T LP++V ++ G YAW T ++ S++ + G+L G + + Sbjct: 31 IMLVALDQTIVGTALPTIVAELKGFELYAWVATAYLLTSVITVPIFGRLGDYFGRKPFVI 90 Query: 77 MALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLAALAYALIPLVF-EPRMWPRAIAL 135 ++ +F + LCG A SM +++ R +QGIGGG+L +A + +F +PR+ R + Sbjct: 91 ASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRLRWQII 150 Query: 136 VSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAAQAWLVWRQLGPATVRSASGPAQ 195 +S +G++T +GP++GG WR F+ LP+ + VWR + +R Sbjct: 151 LSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFV--PHIRHMQHA-- 206 Query: 196 AVPQFLLGRIALL---ASSVLLVALAAQADDDWLASACVLAG--VLLAVWMA------RI 244 G++ L A + L + Q + L V +G VLLAV +A + Sbjct: 207 -------GKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSGMLVLLAVSVAAFYALWKW 259 Query: 245 DLRHRANLLPHAAYRPRTVMG----GVMACIALLVMGSCVEIFLPYFLQGLHGYDPLGAG 300 + R +LP R R + V+A A+ M ++ P QG G P AG Sbjct: 260 EQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSM----LMYAPLLFQGGFGLSPREAG 315 Query: 301 YATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMTLGMALLAFLMPAQWLPAGALADG 360 + A ++ S+ ++GR R I MM LG AL+A L G Sbjct: 316 LLITPLVACITVASI----VNGRIITR-IPNPNLMMNLGFALIAASCLGIVLCDRVTGKG 370 Query: 361 LIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTSSAITTVQLFAMALGAALAG-LVA 419 ++ + G G+G+ P+L A AA + ++ + ++++ LG A+ G LV+ Sbjct: 371 FLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVS 430 Query: 420 NQAGLGAGGA-EATARAAWV 438 G G A E A W+ Sbjct: 431 QMYGAGVEKALEGDHAAQWL 450 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 76/274 (27%), Positives = 125/274 (45%), Gaps = 34/274 (12%) Query: 48 VTLFVAASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGI 107 +T + A + LTG L G +++++ +FG + LCG A S+G ++A R +QG+ Sbjct: 74 ITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGL 133 Query: 108 GGGLLAALAYALIPLVFEPRMWPRAIALVSGMWGVSTLL----GPAVGGLFAASGHWRVA 163 GG L L+ L+ +F P A+ G+W ++TL+ GP +GG+ W Sbjct: 134 AGGPLMPLSQTLLLRIFPKEKAPAAV----GLWAMTTLVAPIAGPILGGVLCDQYSWPYI 189 Query: 164 FFALLPLLAAQAWLVWRQLGPATVRSASGPAQAVPQFLLGRIALLASSVLLVALAAQADD 223 F+ +P+ ++ WR L + + P + R+ L+ V + AL D+ Sbjct: 190 FWINVPVALICGYIGWRMLKRYETKMSKLP--------IDRVGLVLMVVWVAALQLMLDE 241 Query: 224 ----DWLASACVLAGVLLAV-----WMARI--DLRHRANLLPHAAYRPRTVMGGVMA-CI 271 DW AS + VLLAV ++A + +L R ++ +R R V C+ Sbjct: 242 GKEKDWFASTEI---VLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICL 298 Query: 272 AL-LVMGSCVEIFLPYFLQGLHGYDPLGAGYATA 304 A GS V P +LQG GY +G +A Sbjct: 299 AFGAFFGSVV--LTPLWLQGYMGYTATWSGCISA 330 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 84/306 (27%), Positives = 142/306 (46%), Gaps = 31/306 (10%) Query: 17 VALYAINVHIVTTILPSVVRDIG-GLAYYAWNVTLFVAASIVGSALTGKLMGAMGARATY 75 V + ++ I +P++ ++G + W +T F AA+ + LTG L +GA + Sbjct: 33 VFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLF 92 Query: 76 LMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLAALAYALIPLVFEPRMWPRAIAL 135 + ++ LF + LCG A ++ +LA R +QG+ G + L+ +L+ + A+AL Sbjct: 93 VTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALAL 152 Query: 136 VSGMWGVSTLL----GPAVGGLFAASGHWRVAFFALLP--LLAAQAWLVWRQLGPATVRS 189 WG++ ++ GPA+GG S W F+ +P L+AA L A RS Sbjct: 153 ----WGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL-------AIYRS 201 Query: 190 ASGPAQAVPQFLLGRIALLASSV--LLVALAAQADDDWLASACVLA-GVLLAVWMARIDL 246 P +P +G +ALL + V + L D DW AS ++ G++ + A + Sbjct: 202 RETPTHKLPIDKIG-LALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVV 260 Query: 247 RHRANLLPHAAYR---PRTVMGGVMACIAL---LVMGSCVEIFLPYFLQGLHGYDPLGAG 300 N P R R GG +A I++ + G+ V LP +LQ GY + +G Sbjct: 261 WELNNDHPVVDLRLFGRRNFAGGTIA-ISIGYGVFFGNLV--ILPQWLQQYLGYRSIDSG 317 Query: 301 YATAAM 306 +TA + Sbjct: 318 LSTAPI 323 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 58.9 bits (141), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 71/229 (31%), Positives = 103/229 (44%), Gaps = 17/229 (7%) Query: 11 VALTG--GVALYAINVHIVTTILPSVVRDIG-GLAYYAWNVTLFVAASIVGSALTGKLMG 67 + LTG G A+ ++ ++V ILP+V RD+G + W V+ +V G Sbjct: 37 ILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATAD 96 Query: 68 AMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLAALAYALIPLVF-EP 126 G R L +ALF +L C A S + RA+QG+G L A A A+I F + Sbjct: 97 RYGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDE 156 Query: 127 RMWPRAIALVSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAAQAWLVWRQLGPAT 186 RA AL G+ G++ +L P +GG+ WR AF LP A VWR + P + Sbjct: 157 DQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHV-PES 215 Query: 187 VRSASGPAQAVPQFLLGRIALLASS----VLLVALAAQADDDWLASACV 231 P L + +L+SS +L AL + W + ACV Sbjct: 216 RNPLPRP--------LDPLGILSSSSAVFLLTWALITGPEHGWTSVACV 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,064,741 Number of extensions: 37355 Number of successful extensions: 338 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 17 Length of query: 461 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 373 Effective length of database: 1,203,190 Effective search space: 448789870 Effective search space used: 448789870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)