BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0094.p (589 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364... 897 0.0 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 625 e-180 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 206 3e-54 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 197 1e-51 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 192 7e-50 >gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364 [Herbaspirillum seropedicae SmR1] Length = 606 Score = 897 bits (2317), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/595 (74%), Positives = 505/595 (84%), Gaps = 9/595 (1%) Query: 1 MHADILRLTTPLTGQAGQAERERERLAVLDTYCVLDTHPDVLCDLVTELAATLFDTEIAL 60 MHAD+L+ + LT E++RLAVLD YC+LDT PD LCD++TELA LFD EIAL Sbjct: 1 MHADLLKQSPLLTPPLPLDAAEQQRLAVLDAYCLLDTQPDPLCDMITELATRLFDVEIAL 60 Query: 61 VSLVAENRQWFKSRRGLDAPGTPRSQSFCSHVIERDQVMVVLDATQDLRFADNPLVTGEP 120 VSLVAE+RQWFKSRRGLDAPGTPRSQSFC+H I + Q MV+ DA QD RFA NPLV GEP Sbjct: 61 VSLVAEHRQWFKSRRGLDAPGTPRSQSFCAHAIVQAQTMVIPDARQDERFAGNPLVLGEP 120 Query: 121 GIRFYAGAPLQASDGVRLGTLCVIGKSPRTAFGDADRTKLTQLAAIVMARLEILRVSSYV 180 GIRFYAGA L+ +DGV LGTLC+IGKSPR+ F + D+ +L +LAAIVMARL++LR+ SYV Sbjct: 121 GIRFYAGAALRTADGVALGTLCLIGKSPRSQFSEVDQDRLERLAAIVMARLDMLRMGSYV 180 Query: 181 DSLTSLPNRSRLTADLHSLAALGQDGEGLSG------KLTAVAVDICDRVYLRDMIKALG 234 DSLTSLPNRSRL AD+ + A GL+G +L AVAVD+CD YL DM+KALG Sbjct: 181 DSLTSLPNRSRLVADVQLMQA---HATGLAGLDELPRRLAAVAVDVCDHTYLTDMVKALG 237 Query: 235 WDYVEGFLIQAKEQLRQSLGEVALYRIGTTTFAYLERRDIWQQQVEQIERAFAGTVEYQG 294 WDYVEGFLIQAKE+L Q+LGE+ALYRIG T+FAYL+R D WQ Q ++I +AFA TVE+QG Sbjct: 238 WDYVEGFLIQAKERLCQALGEMALYRIGATSFAYLDRCDRWQDQADRIRQAFAATVEHQG 297 Query: 295 IPHRLEISVGIVPLGQCDDIEELVPSLLTAADMARDCGSLQCRYEQPLGQQQKHSFELLS 354 IPH LE+SVGIVPL QC D ELVPSLLTAAD+AR G LQC+YEQPLGQ QKHSFELLS Sbjct: 298 IPHHLEVSVGIVPLSQCPDAAELVPSLLTAADVARADGHLQCQYEQPLGQMQKHSFELLS 357 Query: 355 AIPGALLAGDQLWLEYQPKIALASGHCVGVEALLRWRHPVHGLISPALFIPLAEKTALIR 414 +IP ALL+ DQLWLE+QPK++L++G C GVEALLRWRHP+HG+ISPALFIPLAEKTALIR Sbjct: 358 SIPAALLSTDQLWLEFQPKVSLSTGRCAGVEALLRWRHPIHGVISPALFIPLAEKTALIR 417 Query: 415 QVTLWVLQHGIEQAAAWARRGRAVPVAINVSARDFDNDDLVQTLAIQLRRHRIPAGLIEI 474 QVTLWVLQ GIEQA AWARRG A+PVAINVSARDFDNDDL+Q L L +HRIPAGLIEI Sbjct: 418 QVTLWVLQRGIEQATAWARRGVAIPVAINVSARDFDNDDLLQLLGRLLEQHRIPAGLIEI 477 Query: 475 EFTESAMSKNPEALYGRIQEIKRLGVKVAIDDFGAGFSNLSYLKNIPADYLKIDQSFIRT 534 EFTESAMS+NPE LYGRIQEIKRLGVKVAIDDFGAGFSNLSYLK+IPADYLK+DQSFI Sbjct: 478 EFTESAMSRNPEKLYGRIQEIKRLGVKVAIDDFGAGFSNLSYLKHIPADYLKLDQSFILR 537 Query: 535 MESNYSDQQIVPSMIHLGQKLGFAVVAEGVETEKCRRILHHLGCEYGQGYGIARP 589 MES+YSDQQIVPSMIHLGQ+LGFAVVAEGVE EK RRIL HLGC++GQG+GIARP Sbjct: 538 MESDYSDQQIVPSMIHLGQRLGFAVVAEGVENEKTRRILQHLGCDFGQGFGIARP 592 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 625 bits (1613), Expect = e-180, Method: Compositional matrix adjust. Identities = 314/571 (54%), Positives = 405/571 (70%), Gaps = 5/571 (0%) Query: 21 RERERLAVLDTYCVLDTHPDVLCDLVTELAATLFDTEIALVSLVAENRQWFKSRRGLDAP 80 +E +RLA L YC LDT PD LCD VT+LA ++FD EIALV+LV E+RQWFKSR GL+ Sbjct: 17 QESQRLAQLHGYCALDTPPDKLCDQVTQLACSMFDVEIALVALVDEHRQWFKSRVGLEVS 76 Query: 81 GTPRSQSFCSHVIERDQVMVVLDATQDLRFADNPLVTGEPGIRFYAGAPLQASDGVRLGT 140 TPR+ SFC+H I+ MVV DA QD RF+ + LVTG P IRFYAGAPL G+ LGT Sbjct: 77 ETPRADSFCNHAIQTRHGMVVTDALQDARFSRHALVTGAPFIRFYAGAPLVTPQGLSLGT 136 Query: 141 LCVIGKSPRTAFGDADRTKLTQLAAIVMARLEILRVSSYVDSLTSLPNRSRLTADLHSLA 200 LC+I PR FG + +L QLAAIVM RLE LR Y+D +T+LPNR+RL + L Sbjct: 137 LCIIDSRPRPDFGASQFAQLNQLAAIVMERLEQLRNRGYIDHVTALPNRARL---MEVLR 193 Query: 201 ALGQDGEGLSGKLTAVAVDICDRVYLRDMIKALGWDYVEGFLIQAKEQLRQSLGEVALYR 260 G G SG AVAVDIC R YL DM+KALGWDY EGFL++ LR+ LG + LYR Sbjct: 194 VRGHARPGASGADAAVAVDICPREYLFDMVKALGWDYAEGFLMEVSRTLRRVLGTLPLYR 253 Query: 261 IGTTTFAYLERRDIWQQQ--VEQIERAFAGTVEYQGIPHRLEISVGIVPLGQCDDIEELV 318 + TTTF YL+ Q++ + IE+ F +E+QGIPH L+I++G++P+ + ++V Sbjct: 254 VTTTTFVYLDETAPAQRRRRIADIEQRFTPALEFQGIPHLLDIAIGVLPMEEDVSGGDVV 313 Query: 319 PSLLTAADMARDCGSLQCRYEQPLGQQQKHSFELLSAIPGALLAGDQLWLEYQPKIALAS 378 SLLT D++R+ G +E ++Q+++F LL+A+P AL D+L L +QPK+ L Sbjct: 314 RSLLTVIDVSREEGVSPRLFESVHAEKQRYAFWLLAALPMALTTPDELVLHFQPKVRLRD 373 Query: 379 GHCVGVEALLRWRHPVHGLISPALFIPLAEKTALIRQVTLWVLQHGIEQAAAWARRGRAV 438 G CVGVEAL+RW HP+HG I P +FIPLAEKT IRQVT WVL+ + Q A W + GRA+ Sbjct: 374 GACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVVAQVARWLKSGRAL 433 Query: 439 PVAINVSARDFDNDDLVQTLAIQLRRHRIPAGLIEIEFTESAMSKNPEALYGRIQEIKRL 498 PVA N SARDFD+D+L + LA QL+ H++P LIEIEFTE AM+ +P+ + R+Q ++ L Sbjct: 434 PVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANHPQQVRQRVQALREL 493 Query: 499 GVKVAIDDFGAGFSNLSYLKNIPADYLKIDQSFIRTMESNYSDQQIVPSMIHLGQKLGFA 558 GVKVAIDDFGAGFSNLSYLKNIPAD +KID+SFI + + +DQ IVPS++HL LGF+ Sbjct: 494 GVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIVPSILHLAHNLGFS 553 Query: 559 VVAEGVETEKCRRILHHLGCEYGQGYGIARP 589 VVAEG+E+E+ +L HLGC+YGQGY IARP Sbjct: 554 VVAEGLESERTATLLRHLGCDYGQGYAIARP 584 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 206 bits (525), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 144/431 (33%), Positives = 220/431 (51%), Gaps = 20/431 (4%) Query: 172 EILRVSSYVDSLTSLPNRSRLTADLHSLAALGQDGEGLSGKLTAVAVDICDRVYLRDMIK 231 E +R ++ D+LT LPNR+ L L AL L K T A+ D + + + Sbjct: 593 ERIRELAFFDALTRLPNRTLLLDRLKQAIALS-----LRTK-TCAALLFIDLDHFKTLND 646 Query: 232 ALGWDYVEGFLIQAKEQLRQSLGEV-ALYRIGTTTFAY-LERRDIWQQQV--------EQ 281 +G + + L Q ++L ++ E + R+G F LE + QQ+ E+ Sbjct: 647 TIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEK 706 Query: 282 IERAFAGTVEYQGIPHRLEISVG-IVPLGQCDDIEELVPSLLTAADMARDCGSLQCRYEQ 340 I A T + + +R S+G V LG+ ++EL+ A A++ G R+ Sbjct: 707 ILTALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFD 766 Query: 341 PLGQQQKHSFELLSAIPGALLAGDQLWLEYQPKIALASGHCVGVEALLRWRHPVHGLISP 400 P Q L A + G+QL L YQP++ + G G E L+RW+HP G++ P Sbjct: 767 PAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPP 825 Query: 401 ALFIPLAEKTALIRQVTLWVLQHGIEQAAAWARRGRA--VPVAINVSARDFDNDDLVQTL 458 FIPLAE+T LI + WVL+ Q A WA + + + +A+NVSA D VQT+ Sbjct: 826 GDFIPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTV 885 Query: 459 AIQLRRHRIPAGLIEIEFTESAMSKNPEALYGRIQEIKRLGVKVAIDDFGAGFSNLSYLK 518 + R +++E TES + N E + ++ +K GV+ ++DDFG G+S+LSYLK Sbjct: 886 LDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLK 945 Query: 519 NIPADYLKIDQSFIRTMESNYSDQQIVPSMIHLGQKLGFAVVAEGVETEKCRRILHHLGC 578 +P + LKIDQSF+R + + +D I ++++L Q LG V+AEGVET+ R L GC Sbjct: 946 RLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGC 1005 Query: 579 EYGQGYGIARP 589 QGY RP Sbjct: 1006 HAYQGYFFCRP 1016 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 197 bits (502), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 148/434 (34%), Positives = 218/434 (50%), Gaps = 22/434 (5%) Query: 170 RLEI----LRVS--SYVDSLTSLPNRSRLTADLHSLAALGQDGEGLSGKLTAVAVDICDR 223 RLEI L++S +Y D LT LPNR + L A G + + + +D + Sbjct: 345 RLEIASRELKISRLAYQDQLTGLPNRVQFADLLRDAIAEAARTPGAACYILMMDLDRFQQ 404 Query: 224 VYLRDMIKALGWDYVEGFLIQAKEQLRQSLGE--VALYRIGTTTFAYL---ERRDIWQQQ 278 V LG + + L Q +L L L R+G FA L ++ +Q Sbjct: 405 VN-----DVLGHSFGDDLLRQVALRLSAQLLHPGAKLSRLGGDEFAILLPGVDQEGAVEQ 459 Query: 279 VEQIERAFAGTVEYQGIPHRLEISVGIVPL---GQCDDIEELVPSLLTAADMARDCGSLQ 335 QI RA + L +GI GQ D+ L+ A +A+ GS Sbjct: 460 ALQILRALEQPLSLHQQTVDLGAGIGISGYPAHGQSSDV--LLSHAEVAMYIAKRKGSGL 517 Query: 336 CRYEQPLGQQQKHSFELLSAIPGALLAGDQLWLEYQPKIALASGHCVGVEALLRWRHPVH 395 Y+ + Q + S LLS + AL D+ L QPK+ L SG VG EAL+RW HP Sbjct: 518 VIYDPAIDQSSQESLSLLSELRRAL-ERDEFMLYLQPKLELCSGQVVGAEALVRWIHPEK 576 Query: 396 GLISPALFIPLAEKTALIRQVTLWVLQHGIEQAAAWARRGRAVPVAINVSARDFDNDDLV 455 G I P FIP AE T IR +TLW+++ + ++G + ++N+S RD + +L Sbjct: 577 GFIGPDNFIPFAETTGFIRMLTLWMMEQVARLSEQLQQQGVQLKFSVNLSTRDLLDQELP 636 Query: 456 QTLAIQLRRHRIPAGLIEIEFTESAMSKNPEALYGRIQEIKRLGVKVAIDDFGAGFSNLS 515 A L+RHR+ + +E TESA+ +P ++ + +G ++IDDFG G+S+L+ Sbjct: 637 TAFAAILQRHRLQPAALCLEITESAIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLA 696 Query: 516 YLKNIPADYLKIDQSFIRTMESNYSDQQIVPSMIHLGQKLGFAVVAEGVETEKCRRILHH 575 YLK +P D LKID+SF+ M + D +IV S + LG LG VVAEGVE+E+ +L Sbjct: 697 YLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQ 756 Query: 576 LGCEYGQGYGIARP 589 +GC+ GQGY + +P Sbjct: 757 MGCDQGQGYFMGKP 770 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 192 bits (488), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 161/545 (29%), Positives = 247/545 (45%), Gaps = 42/545 (7%) Query: 75 RGLDAPGTPRSQS-------------FCSHVIERDQVMVVLDATQDLRFADNPLVTGEPG 121 R L AP P + S C Q ++ D D + D + G Sbjct: 437 RTLAAPSLPEAYSKAIDGLHVGPMVGCCGSAAYTGQTVISEDIQTDPHWNDYKHLALPHG 496 Query: 122 IRFYAGAPLQASDGVRLGTLCVIGKSP--RTAFGDADRTKLTQLAAIVM----ARLEILR 175 +R P++++ G +GT + P +AF L A+ + AR I + Sbjct: 497 LRACWSTPIKSASGQVIGTFGFYYRHPGQPSAFHHLLVEAGLHLCALAIEREDARARIHQ 556 Query: 176 VSSYVDSLTSLPNRSRLTADLHSLAALGQDGEGLSGKLTAVAVDICDRVYLRDMIKALGW 235 ++ Y DSLT LPNRS L L GQ + VAV D + + +LG Sbjct: 557 LAFY-DSLTGLPNRSLL------LVQAGQAIAAAERNCSTVAVMFIDLDRFKQVNDSLGH 609 Query: 236 DYVEGFL--IQAKEQLRQSLGEVALYRIGTTTFAYLERRDIWQ--QQVEQIERAFAGTVE 291 + L + + Q + ++ G L + D + V++I+ A Sbjct: 610 QAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTQHLTEVVKRIQERLAMPCH 669 Query: 292 YQGIPHRLEISVGIVPL-GQCDDIEELVPSLLTAADMARDCGSLQCR-----YEQPLGQQ 345 G P S+G+ +DIE +LL AD+A R + + + Sbjct: 670 INGTPLNPSASIGVAIFPDNGNDIE----TLLHRADIAMYQAKAVNRGRFSFFSEEMNSM 725 Query: 346 QKHSFELLSAIPGALLAGDQLWLEYQPKIALASGHCVGVEALLRWRHPVHGLISPALFIP 405 + L SA+ AL GD L L +QP+I L GVEAL RW HP G ISP FIP Sbjct: 726 AQERLALESALRDALRGGD-LELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIP 784 Query: 406 LAEKTALIRQVTLWVLQHGIEQAAAWARRGRAVP-VAINVSARDFDNDDLVQTLAIQLRR 464 +AE+ LI ++ W L+ Q + W RRG VP +++N+SA +F + DL Q + LRR Sbjct: 785 IAEECGLIGELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFVTGLLRR 844 Query: 465 HRIPAGLIEIEFTESAMSKNPEALYGRIQEIKRLGVKVAIDDFGAGFSNLSYLKNIPADY 524 + + + +E TE+ + + I + LG+ +A+DDFG G+S+L YL+ +P Sbjct: 845 YALEPSDLTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSE 904 Query: 525 LKIDQSFIRTMESNYSDQQIVPSMIHLGQKLGFAVVAEGVETEKCRRILHHLGCEYGQGY 584 LK+DQSF+R M + + + ++I +G+ L VVAEGVE + +L GC+ QGY Sbjct: 905 LKLDQSFVRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGY 964 Query: 585 GIARP 589 +RP Sbjct: 965 LFSRP 969 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,880,312 Number of extensions: 80980 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 23 Length of query: 589 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 498 Effective length of database: 1,188,985 Effective search space: 592114530 Effective search space used: 592114530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)