BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0081.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 734 0.0 gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 573 e-165 gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 75 9e-15 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 734 bits (1894), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/420 (88%), Positives = 401/420 (95%) Query: 1 MSFQQGLSGLQGASKHLEVVGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 MSFQQGLSGL GASKHL+V+GNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT Sbjct: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 Query: 61 LANVSQAFTQGNIATTNNALDVAINGDGFFRMAANLNDQSPRYGRNGQFQLNKDGYLINP 120 +ANVSQAFTQGNIATTNNALDVAINGDGFFRMAA+L DQSPRYGRNGQFQLNKDGYLINP Sbjct: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 Query: 121 SMNGAYLTGYPAGAAGGDPVPLKIDTATLPATATSRITTKINLDSRKSVPTISPFDPANP 180 SMNGAYLTGYPAG GGDPVPLKIDTATLPATAT+R++TK+NLDSRKSVPT+SPFDPANP Sbjct: 121 SMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP 180 Query: 181 NSYNNSTGATVYDSLGNPYSLQTYYVKSPPTGSPPATTWSVYGTLDGKLINAAPPATGPQ 240 NSY+NSTGATVYDSLGNPYSLQT+YVK TG+PP+TTWSVY TLDGK I APPATGPQ Sbjct: 181 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ 240 Query: 241 LLGTLRFDATGALTASTGMTVDLSSMVKGGASFPTPIALNYAGSTQTGSAFANLAQSQDG 300 LLGTLRFDATGALTASTGM+++LS++ KGGA+FP PIAL YAGSTQTGSAFANLAQSQDG Sbjct: 241 LLGTLRFDATGALTASTGMSLNLSTLAKGGATFPGPIALGYAGSTQTGSAFANLAQSQDG 300 Query: 301 SPPGTLSSFSIGKDGSLVGSYSNQQTKVLGQIVLVNFANPNGLQPLGNNLYQATAAAGSP 360 PPGTLSSFSIGKDGS++G+YSNQQT+V+GQIVLVNFANP+GLQP+GNNLYQA+ AAGS Sbjct: 301 MPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASEAAGSA 360 Query: 361 LVGTPTTATFGSLQGRAVEQSNVDLTAELVNMIVAQRVYQANSQSIKVQDTVLQTLISLR 420 LVGTPTT FG+LQGRAVEQSNVDLTAELVNMIVAQRVYQANSQ+IKVQDTVLQTL+SLR Sbjct: 361 LVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSLR 420 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 573 bits (1477), Expect = e-165, Method: Compositional matrix adjust. Identities = 300/433 (69%), Positives = 357/433 (82%), Gaps = 14/433 (3%) Query: 1 MSFQQGLSGLQGASKHLEVVGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 M FQQGLSGL GA+K L+V+GNNVANASTVGFKQ Q QFAD YANSMNRSGNSPVGIGV+ Sbjct: 1 MGFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNSPVGIGVS 60 Query: 61 LANVSQAFTQGNIATTNNALDVAINGDGFFRMAANLNDQSPRYGRNGQFQLNKDGYLINP 120 + V+Q FTQGNI+++NN LD+AINGDGFF+++A+L ++SP YGRNGQFQL+K GY++NP Sbjct: 61 VPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLDKSGYIVNP 120 Query: 121 SMNGAYLTGYPAGAAGGDPVPLKIDTATLPATATSRITTKINLDSRKSVPTISPFDPANP 180 SMNGAYL G+ AGA GGDPVPL+IDT+++PAT T+ I+TK+NLDSR SVPT +PF+ A+P Sbjct: 121 SMNGAYLMGWAAGAQGGDPVPLQIDTSSIPATPTTTISTKVNLDSRLSVPTTTPFNAADP 180 Query: 181 NSYNNSTGATVYDSLGNPYSLQTYYVKSPPTGSPPATTWSVYGTLDG-KLINAAPPATGP 239 SYN+STG +YDSLGNPYS+QTYYVK+ PTG+PP TTW+VY +D L N A PA P Sbjct: 181 TSYNSSTGVDLYDSLGNPYSMQTYYVKAAPTGTPPTTTWTVYAKIDNTTLNNGATPAV-P 239 Query: 240 QLLGTLRFDATGALTASTGM------------TVDLSSMVKGGASFPTPIALNYAGSTQT 287 LGT+ FD+TG +T G T L+++ K G F PI ++Y+GSTQT Sbjct: 240 VALGTMTFDSTGTMTQINGAAISPTNNTLTISTAQLATVAKAGGVFAAPINISYSGSTQT 299 Query: 288 GSAFANLAQSQDGSPPGTLSSFSIGKDGSLVGSYSNQQTKVLGQIVLVNFANPNGLQPLG 347 GSAF NLAQ Q+G PPG+LSSFS+ KDGS++GSYSNQQTK LG +VLVNFANPNGLQPLG Sbjct: 300 GSAFVNLAQVQNGMPPGSLSSFSVDKDGSIIGSYSNQQTKNLGTVVLVNFANPNGLQPLG 359 Query: 348 NNLYQATAAAGSPLVGTPTTATFGSLQGRAVEQSNVDLTAELVNMIVAQRVYQANSQSIK 407 NNLYQA++AAGSPLVG PTT TFG+LQ RA E SNVDLTAELVNMIVAQRVYQANSQ+IK Sbjct: 360 NNLYQASSAAGSPLVGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTIK 419 Query: 408 VQDTVLQTLISLR 420 VQDTVLQTL+SLR Sbjct: 420 VQDTVLQTLVSLR 432 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 75.1 bits (183), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 50/124 (40%), Positives = 71/124 (57%), Gaps = 7/124 (5%) Query: 302 PPGTL----SSFSIGKDG--SLVGSYSNQQTKVLGQIVLVNFANPNGLQPLGNNLYQATA 355 PP T+ + ++G+DG S++ + S T++ G I + F NP GL+ G NLY TA Sbjct: 138 PPITVPLNTTKLTVGRDGVVSIMQAGSTATTQI-GTIQVATFINPAGLESRGENLYMETA 196 Query: 356 AAGSPLVGTPTTATFGSLQGRAVEQSNVDLTAELVNMIVAQRVYQANSQSIKVQDTVLQT 415 A+G+P P T G+L VE SNV++ ELVNMI QR Y+ NS++I D +L Sbjct: 197 ASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAK 256 Query: 416 LISL 419 L L Sbjct: 257 LSQL 260 Score = 72.0 bits (175), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 44/125 (35%), Positives = 64/125 (51%), Gaps = 13/125 (10%) Query: 8 SGLQGASKHLEVVGNNVANASTVGFKQGQVQFADAYANSMNRSGNSP-----------VG 56 +GL L+V+ NN+AN ST GFK+ + F D ++ + G +G Sbjct: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 Query: 57 IGVTLANVSQAFTQGNIATTNNALDVAINGDGFFRMAANLNDQSPRYGRNGQFQLNKDGY 116 GV + FTQGN+ TNN D+AI G+GFF++ L D + Y R+G FQ + G Sbjct: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQIL--LPDGTTAYTRDGAFQTDSQGQ 127 Query: 117 LINPS 121 L+ S Sbjct: 128 LVTSS 132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.128 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,292,526 Number of extensions: 55872 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 5 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)