BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0075.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 713 0.0 gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 279 3e-76 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 154 1e-38 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 139 4e-34 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 119 7e-28 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 713 bits (1841), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/384 (91%), Positives = 371/384 (96%) Query: 28 GWCVSYIDRAAITFAATQIASEFALKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV 87 GWCVSYIDRAAITFAATQIASEF LKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV Sbjct: 21 GWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV 80 Query: 88 IVISILLWSVFTGVTGMAWSITSLVVIRFIFGLGEGAFPAASVKGVAENFSREERPKMSS 147 IVISILLWS+FTGVTGMAWS+TSLV+IRFIFG+GEGAFPAASVKGVAENFSR+ERPKMSS Sbjct: 81 IVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKGVAENFSRDERPKMSS 140 Query: 148 LLMSSNYIGSMLAPLLIAPLIMHFGWRAVFHTIGIAGLVFALVYWFCVRPVRPGKDGPKA 207 LLMSSNYIGSMLAPLLIAPLI+H+GWRAVFH IGIAG+VFALVYWFCVRPVRPG G A Sbjct: 141 LLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYWFCVRPVRPGVGGAGA 200 Query: 208 INKEAFAALLRMPLMWKIVAVWFGLSIINKGLDSWMPTYLMTVRGLNLKAVGLLLPLPYI 267 INK+AF AL++MPLMW+IVAVWFGLSIINKGLD+WMPTYLMTVRGLNLKAVGLLLPLPYI Sbjct: 201 INKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYI 260 Query: 268 MAGLSTAIGGWVMVRFFDGREKLLLIASSLLTAVFVYCMYTAVTVEGVIAWQCLAYFFKS 327 MAGLSTAIGGWVMVRFFDGREKLLLI SSLLTAVFVYCMY+A +VEGVIAWQCLAYFFKS Sbjct: 261 MAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMYSAASVEGVIAWQCLAYFFKS 320 Query: 328 FVLATCIALPTKLLQAQMVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIG 387 FVLATCIALPTK+LQAQ+VGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIG Sbjct: 321 FVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIG 380 Query: 388 AALFSVLVSLFIRTSKTGGQMQPA 411 AA FSVLVSLFIRT K+ QMQPA Sbjct: 381 AAFFSVLVSLFIRTHKSDSQMQPA 404 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 279 bits (713), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 151/396 (38%), Positives = 243/396 (61%), Gaps = 7/396 (1%) Query: 7 AMTKAQGEGRYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFALKPSDLGILLSSFFL 66 A T+AQ K + L L+L W V Y DR ++ A I+ EFAL G++LS+F++ Sbjct: 4 ATTRAQ-----KMICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYV 58 Query: 67 GYSLLQLPGGWLADRYGSRPVIVISILLWSVFTGVTGMAWSITSLVVIRFIFGLGEGAFP 126 Y+L+QL GGWL+DRYGSR V+V+ ++ WS+FTG+T MAWS SL++IRF+FG GEG F Sbjct: 59 SYALMQLMGGWLSDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFS 118 Query: 127 AASVKGVAENFSREERPKMSSLLMSSNYIGSMLAPLLIAPLIMHFGWRAVFHTIGIAGLV 186 AS VAE F ++ER + S L+S+ ++G+ + ++A +++ GWR FH + G++ Sbjct: 119 PASSVTVAEVFPQQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMI 178 Query: 187 FALVYWFCVRP-VRPGKDGPKAINKEAFAALLRMPLMWKIVAVWFGLSIINKGLDSWMPT 245 A + W +R + + A K L++ P ++ A+WF SI+ G+ SWMP+ Sbjct: 179 VAAILWLALRSGMAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPS 238 Query: 246 YLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLIASSLLTAVFVYC 305 +L+ V ++L +G+ +PY++A L T + GW++ + GREK ++ +L AVF+ Sbjct: 239 FLLKVYQIDLLHIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYFMMGGALACAVFLAL 298 Query: 306 MYTAVTVEGVIAWQCLAYFFKSFVLATCIALPTKLLQAQMVGSGVGMVNLGGQLAGFISP 365 M + +E ++ + L +FV AT ++P K + ++GS G++N GGQLAG I+P Sbjct: 299 MISTTVIELLVVYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAP 358 Query: 366 LVIGFLVSAFAN-YDYAFSFLIGAALFSVLVSLFIR 400 +V+G L+ AF Y AF FL+G+A+ S L+++ R Sbjct: 359 VVMGSLIVAFNGAYLAAFWFLVGSAMMSFLIAMTWR 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 154 bits (389), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 129/425 (30%), Positives = 214/425 (50%), Gaps = 52/425 (12%) Query: 18 KYLVLVLLYLGWCVSYIDRAAITFAATQIASEFALKPSDLGILLSSFFLGYSLLQLPGGW 77 +YL+L LL++ V+Y DRA ++ A + + +E +L P +G + S+F Y L QLPGGW Sbjct: 12 RYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGW 71 Query: 78 LADRYGSRPVIVISILLWSVFT----GVTGM--AWSITSLVVIRFIFGLGEG-AFPAASV 130 L DR+GS+ + + SI LWSVFT GVT M A ++ SL ++RF+ GL E +FPA Sbjct: 72 LLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPANG- 130 Query: 131 KGVAENFSREERPKMSSLLMSSNYIGSMLAPLLIAPLIMHFGWRAVFHTIGIAGLVFALV 190 + VA F +ER S++ S+ Y ++L L+A ++ + W VF +G+ G+ AL+ Sbjct: 131 RIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALI 190 Query: 191 YWFCVRPVRPGKDGPKAINKEAFAAL------------------------LRMPLMWK-I 225 + R + KD P+ IN+ A + +R L + + Sbjct: 191 WG---RIIYSPKDHPR-INQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRML 246 Query: 226 VAVWFG---LSIINKGLDSWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVR 282 + V+ G +++I +W P YL+ RG+++ G + +P + + +GG + R Sbjct: 247 LGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDR 306 Query: 283 FFDG------REKLLLIASSLLTAVFVYCMYTAVT--VEGVIAWQCLAYFFKSFVLATCI 334 K+ ++ LL+ + C Y V G++A LA+F K V A Sbjct: 307 LIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMA---LAFFGKG-VGALGW 362 Query: 335 ALPTKLLQAQMVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAALFSVL 394 A+ Q+VG G+ N+ G +AG +P+VIG++V ++ A F+ AL +V+ Sbjct: 363 AVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALVFVGINALVTVI 422 Query: 395 VSLFI 399 L I Sbjct: 423 SYLVI 427 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 139 bits (350), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 109/441 (24%), Positives = 206/441 (46%), Gaps = 44/441 (9%) Query: 6 AAMTKAQGEGRYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFALKPSDLGILLSSFF 65 AA+ + + ++++V L+++ + V+ DRA + A + EF + ++ G L S F Sbjct: 4 AAIQHTKKKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFL 63 Query: 66 LGYSLLQLPGGWLADRYGSRPVIVISILLWSVFTGVTGMAWSITSLVVIRFIFGLGEGAF 125 YSL QLP G R+G V +++L SVFTG+ G A S+ +L + RF GL EG Sbjct: 64 FAYSLAQLPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLGLAEGPL 123 Query: 126 PAASVKGVAENFSREERPKMSSLLMSSNYIGSMLAPLLIAPLIMHFGWRAVFHTIGIAGL 185 P + F E+ M+ + +++ G +L P + A ++ ++GWR +F+ + G+ Sbjct: 124 PIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVPGV 183 Query: 186 VFALVYWFCVRPVRP----------------------GKDGPKAINKEAFAALLRMPLMW 223 + ++V W V RP G+D A+ ++ FA L R+ Sbjct: 184 ILSVV-WLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRD-FALLDRIIRRS 241 Query: 224 KIVAV------------------WFGLSIINKGLDSWMPTYLMTVRGLNLKAVGLLLPLP 265 K+ + +F ++ I L +W+PTYL++V+G ++ +G++ P Sbjct: 242 KVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAP 301 Query: 266 YIMAGLSTAIGGWVMVRFFDGREKLLLIASSLLTAV-FVYCMYTAVTVEGVIAWQCLAYF 324 ++ A + +GG + R+ + R K ++ S+ T + V + + A L Sbjct: 302 WVGAVIGNLLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPADPLLYGALLLLTGI 361 Query: 325 FKSFVLATCIALPTKLLQAQMVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSF 384 + +T + P + ++ MVN GQL G +PL GFL+ ++ ++++ + F Sbjct: 362 LLNIGYSTYMVYPMGMTSKKVYPMACAMVNTLGQLGGACAPLAAGFLLDSY-SWNHVWIF 420 Query: 385 LIGAALFSVLVSLFIRTSKTG 405 + ++ + L IR G Sbjct: 421 MAVCSILCFITILTIREPVQG 441 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 119 bits (297), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 109/409 (26%), Positives = 194/409 (47%), Gaps = 27/409 (6%) Query: 16 RYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFALKPSDLGILLSSFFLGYSLLQLPG 75 R + + L L L V+Y+DR+ ++ A ++ E L S +G+LLS+F L Y+ QLP Sbjct: 13 RIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPI 72 Query: 76 GWLADRYGSRPVIVISILLWSVFTGVTGMAWSITSLVVIRFIFGLGEG-AFPAASVKGVA 134 G + D++G+R ++ + +WSV G ++ +V R GLGE FPA + K V+ Sbjct: 73 GAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGA-KVVS 131 Query: 135 ENFSREERPKMSSLLMSSNYIGSMLAPLLIAPLIMHFGWRAVFHTIGIAGLVFALVYWFC 194 E F+ ER + + ++S+ IG LAP ++ L++ FGWR +F +G+ G+ ++ ++ Sbjct: 132 EWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLF 191 Query: 195 VR-----PVRPGK---------DGPKAINK---EAFAALLRMPLMWKIVAVWFGLSIINK 237 R + P + + P A K + +L W ++ + G+ + Sbjct: 192 YRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVW 251 Query: 238 GLDSWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWV----MVRFFDG-REKLLL 292 +W+P YL R L++ G ++ +PYI L G++ M R R + Sbjct: 252 LYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMPAIRSRKWP 311 Query: 293 IASSLL--TAVFVYCMYTAVTVEGVIAWQCLAYFFKSFVLATCIALPTKLLQAQMVGSGV 350 I + L+ A + YT T ++ + LA F + AL + + ++V S Sbjct: 312 ICAGLIGAGAFTIPAAYTPSTTMAIV-YISLAMAFVNMASGGAWALVSVAIPRRLVASLG 370 Query: 351 GMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAALFSVLVSLFI 399 M N GG L G ++P++ G +V ++ A A + LV +F+ Sbjct: 371 SMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFV 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,215,835 Number of extensions: 50414 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 352 Number of HSP's successfully gapped: 38 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)