BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0072.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 850 0.0 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 504 e-144 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 429 e-121 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 358 e-100 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 230 2e-61 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 850 bits (2197), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/500 (85%), Positives = 461/500 (92%), Gaps = 2/500 (0%) Query: 1 MNPSLTHRAAHRPRALMTPLLLAMLLGGCTLMPDYQRPAAPVASQFAGDHSAAAASSTPA 60 M PSL+ R+ P +M P++LAMLLGGCTLMPDY RP APVA+ FAG S AA ++ A Sbjct: 1 MKPSLSTRSPVLP--MMLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSA 58 Query: 61 MAVSDIGWRDVFTDPTLQEVISLSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAAT 120 V+DIGWRDVFTDP+LQ+VI+L+L NNRDLRVAVLNIEKARAQYGV+RAALFP+I AAT Sbjct: 59 PVVADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAAT 118 Query: 121 SESASRTPADLSATGRALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRS 180 SE+ASRTP DLS+TG ALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRS Sbjct: 119 SETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRS 178 Query: 181 TQISLIAEVATAWLTLASDQDRLRLAKDTLASQSSSYELTQRSFELGSSSALTLRQAQTS 240 TQISLIAEVATAWLTLASDQDRLRLA+DTL SQSSSY LTQRSFELGSSSALTLRQAQTS Sbjct: 179 TQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTS 238 Query: 241 VESARVDVESYTAQVDQDRNALVLLVGKDLPDRLLPQGLPDTALAAASPLATIPPGLPSD 300 VESARVDVESYTAQVDQDRNALVLLVGKDLP LLPQGLPDT LAAASPLATIPPGLPSD Sbjct: 239 VESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSD 298 Query: 301 LLQRRADIVEAERTLRAANANIGAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFV 360 LLQRRADIV+AER LRAANANIGAARAAFYPSI+LT +AGTASARLAGLFKGGSGSWSFV Sbjct: 299 LLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFV 358 Query: 361 PQISLPIFDGGANQANLDIATISRDISVAQYEKAIQTAFQEVSDALAQRNTLGRQLKAQE 420 PQISLPIFDGGANQANLDIAT+SRDISVAQYEKAIQTAF+EVSDALAQRNTLGRQL+AQE Sbjct: 359 PQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQE 418 Query: 421 DLVDASDASYRLSQARFNNGVDSYLTVLDSQRTLYSAQKTLIGTRLSRWTNLVTFYKALG 480 LV ASD ++RLSQAR+++GVDSYLTVLDSQRTLYSAQK LIGT+LSRWTNLVTFYK LG Sbjct: 419 ALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLG 478 Query: 481 GGWVERSGSPVAGDLATAAP 500 GGWVER+G+P A D+AT P Sbjct: 479 GGWVERTGTPAASDIATQEP 498 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 504 bits (1297), Expect = e-144, Method: Compositional matrix adjust. Identities = 270/466 (57%), Positives = 340/466 (72%), Gaps = 10/466 (2%) Query: 28 GC-TLMPDYQRPAAPVASQFAGDHSA--AAASSTPAMAVSD---IGWRDVFTDPTLQEVI 81 GC L PDY +PAAPV + + +A A S+ PA D +GWR+ F DP LQ+VI Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 82 SLSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSATGRALTSR 141 + +L NNRDLRVA LNIEKARAQY + A +P+I+A + SASRTPA+LS TG A S Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 142 SYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQD 201 + T+G SAYELD FGR+++L + AL+S+ E RRST ISL+AEVATAWLTLASD+D Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 202 RLRLAKDTLASQSSSYELTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNA 261 L+LA+DT ASQS ++ L+QRS ++G+ S + L + +VESAR DV Y +QV+QD NA Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 262 LVLLVGKDLPDRLLPQGLPDTALAAASPLATIPPGLPSDLLQRRADIVEAERTLRAANAN 321 L LLVG D+ LLP GLPDT AS L P GLPS +L RR D++ AE TL++ANA+ Sbjct: 267 LRLLVGADIDAALLPAGLPDT----ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANAD 322 Query: 322 IGAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIAT 381 IGAARAAF+PSISLTA+ GTAS L GLFK GSGSW+F P I+LPIF+ G+ +A+LD A Sbjct: 323 IGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAK 382 Query: 382 ISRDISVAQYEKAIQTAFQEVSDALAQRNTLGRQLKAQEDLVDASDASYRLSQARFNNGV 441 I+RDI VA YEKAIQTAF+EV+DALA R TL ++L AQ AS+ ++RLS AR+ NGV Sbjct: 383 ITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGV 442 Query: 442 DSYLTVLDSQRTLYSAQKTLIGTRLSRWTNLVTFYKALGGGWVERS 487 DSYLT L +QRTLYSAQ+TLI RL+ +N +T YK LGGGW E++ Sbjct: 443 DSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 429 bits (1102), Expect = e-121, Method: Compositional matrix adjust. Identities = 245/490 (50%), Positives = 316/490 (64%), Gaps = 18/490 (3%) Query: 3 PSLTHRAAHRPRALMTPLLLAMLLGGCTLMPDYQRPAAPVASQFAGDHSAAAASSTPAMA 62 P L H A L++A +LGGC+L P Y+RP APV S + D AA A Sbjct: 10 PRLLHMCA--------ALVMAGVLGGCSLAPTYERPQAPVESSYPAD----AAGRAELQA 57 Query: 63 VSDIGWRDVFTDPTLQEVISLSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAATSE 122 V ++GWR F D LQ++I+ +L NNRDLR AVLNIE+ARAQY + RA L P +N + + Sbjct: 58 V-NLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNA 116 Query: 123 SASRTPADLSATGRALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQ 182 + +RT A S TG+ S SY L AYELD FGRVR+LK+ AL S+LST EA++S Q Sbjct: 117 ARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQ 176 Query: 183 ISLIAEVATAWLTLASDQDRLRLAKDTLASQSSSYELTQRSFELGSSSALTLRQAQTSVE 242 ISL+A+VA A+L S ++L+LA +TL S+ S+ +L Q+ ++G+S+AL R QT VE Sbjct: 177 ISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVE 236 Query: 243 SARVDVESYTAQVDQDRNALVLLVGKDLPDRLLPQGLPDTALAAASPLATIPPGLPSDLL 302 SARV Q Q NAL LLVGK + + LP P L+ + IP GLPSDLL Sbjct: 237 SARVAKTQLERQRAQAENALTLLVGKKIGE--LP---PAQLLSDEKIVTDIPAGLPSDLL 291 Query: 303 QRRADIVEAERTLRAANANIGAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQ 362 R DI AE++L AANANIGAARAAF+P ISLT + G+AS+ L GLF+ GSG+WSF P Sbjct: 292 TNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPS 351 Query: 363 ISLPIFDGGANQANLDIATISRDISVAQYEKAIQTAFQEVSDALAQRNTLGRQLKAQEDL 422 +SLPIFD G N ANLD+A + ++ +V YEKAIQTAF+EV+DAL R +L Q+KAQE Sbjct: 352 LSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAF 411 Query: 423 VDASDASYRLSQARFNNGVDSYLTVLDSQRTLYSAQKTLIGTRLSRWTNLVTFYKALGGG 482 + A +L+ RF NG+ S L LD+QR L+SAQ+ LI R R N + YKALGGG Sbjct: 412 LAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGG 471 Query: 483 WVERSGSPVA 492 E + S A Sbjct: 472 LNENTVSVAA 481 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 212/455 (46%), Positives = 286/455 (62%), Gaps = 7/455 (1%) Query: 29 C-TLMPDYQRPAAPVASQFA-GDHSAAAASSTPAMAVSDIGWRDVFTDPTLQEVISLSLA 86 C +L P YQRPAAPVA+ + G + AA A + WRD FTDP LQ +I+ +L Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 87 NNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSATGRALTSRSYTAT 146 N+RDLR A L +++A+A YG+ A LFPTI A SRTPADL+ T R L +Y A Sbjct: 85 NSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAG 144 Query: 147 LGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLA 206 LG S++E+DL+GR+RSL + AL+S ++ R + +SL+A+VA AWL LA +RL +A Sbjct: 145 LGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIA 204 Query: 207 KDTLASQSSSYELTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLV 266 T+AS+ S+ + R E+G++S L L Q +T + A+ Q Q NAL LLV Sbjct: 205 NQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLV 264 Query: 267 GKDLPDRLLPQGLPDTALAAASPLATIPPGLPSDLLQRRADIVEAERTLRAANANIGAAR 326 G + +LP G L A A + PGLP+ LL RR D+ AE L AANANIGAAR Sbjct: 265 GSPV---VLPVGQARAGLDAV--FAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAAR 319 Query: 327 AAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATISRDI 386 AAF+PSISLT + GTASA L+GLF+ GS +W+F P I+LPIF G + NL ++ + RD+ Sbjct: 320 AAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDL 379 Query: 387 SVAQYEKAIQTAFQEVSDALAQRNTLGRQLKAQEDLVDASDASYRLSQARFNNGVDSYLT 446 +VA YEK +QTAF+EV+DALA R L +QL+ + V RLS R++NG +L Sbjct: 380 AVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLD 439 Query: 447 VLDSQRTLYSAQKTLIGTRLSRWTNLVTFYKALGG 481 VLD+QR L SAQ+ L+ R + ++ V Y ALGG Sbjct: 440 VLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 230 bits (587), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 164/449 (36%), Positives = 237/449 (52%), Gaps = 10/449 (2%) Query: 38 PAAPVASQFAGDHSAAAASSTPAMAV---SDIGWRDVFTDPTLQEVISLSLANNRDLRVA 94 P P A + A PA A S IGW+D F LQ +I+ +L +N DLR Sbjct: 26 PLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFPQAPLQALIAEALRHNPDLRDI 85 Query: 95 VLNIEKARAQYGVERAALFPTINAATSESASRTPADLSATGRALTSRSYTATLGFSAYEL 154 L +++ +AQ+ ++R+ PT+N SR P + +G A ++R Y LG S++EL Sbjct: 86 RLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWEL 145 Query: 155 DLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLAKDTLASQS 214 D +GR+ SL E ALQ ++++ A ++ ISLI +VA AW +L +RL L + A+ + Sbjct: 146 DFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDERLHLLETLQAATA 205 Query: 215 SSYELTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVG-KDLPDR 273 S L Q + G + A L Q ++ + R + R+ L +L G LP Sbjct: 206 ESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMRASQRSTLEMLAGLTQLPAA 265 Query: 274 LLPQGLPDTALAAASPLATIPPGLPSDLLQRRADIVEAERTLRAANANIGAARAAFYPSI 333 L P P T L P A + PGLPS LL R DI+ AE L+A+ A + AARAAF+P I Sbjct: 266 LEPA--PTTILL---PFA-LAPGLPSTLLAARPDIIAAEHRLQASAAQVAAARAAFFPRI 319 Query: 334 SLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATISRDISVAQYEK 393 SLTA G AS L LFKG S +W+FVP+ISLP+ DGG Q+ DIA + + ++ YE+ Sbjct: 320 SLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYER 379 Query: 394 AIQTAFQEVSDALAQRNTLGRQLKAQEDLVDASDASYRLSQARFNNGVDSYLTVLDSQRT 453 +Q AF+EV+ L + + L QL+ + ++V R G S L VL+S+R Sbjct: 380 TVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERD 439 Query: 454 LYSAQKTLIGTRLSRWTNLVTFYKALGGG 482 AQ+ + N + Y ALGGG Sbjct: 440 HLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,312,504 Number of extensions: 48548 Number of successful extensions: 333 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 280 Number of HSP's successfully gapped: 18 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)