BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0070.p (394 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 694 0.0 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 394 e-110 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 347 8e-97 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 141 9e-35 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 127 1e-30 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 694 bits (1791), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/385 (92%), Positives = 375/385 (97%), Gaps = 3/385 (0%) Query: 1 MRTTPLLRPVAAL---AILSVLSACGKPPGGPPPAAGTPVLGVMTVRAQPVELHTELPGR 57 MRT P+ +PVAAL AILSVLSACGKPPGG PPAAGTPVLGV+TV AQPVELHTELPGR Sbjct: 1 MRTPPIPKPVAALTALAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGR 60 Query: 58 TSPYQIADVRPQVNGIIKARNFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV 117 TSPYQIADVRPQVNGIIKAR+FREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV Sbjct: 61 TSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV 120 Query: 118 KTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYAKVDAP 177 +TARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYA++DAP Sbjct: 121 RTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAP 180 Query: 178 ISGRIGKSSVTPGALVTASQTTALTTIQQLDPIYVDVTQSSTNWLNLKEALANGELQKSG 237 ISGRIGKSSVTPGALVTA+QTTALTTIQQLDPIYVDVTQSST WLNLKEALANG+L+KSG Sbjct: 181 ISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG 240 Query: 238 NGAKVRLILENGRTYAQEGTLQFSDVTVNQDTGAITLRAVFPNPKTDLLPGMYVRAVLQE 297 NGAKVRL+LENGRTYAQEGTLQFSDVTVNQDTGAITLRA+FPNPKTDLLPGMYVRAVLQE Sbjct: 241 NGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQE 300 Query: 298 GVRQQGLLVPQQAVSRDGAGKPVAFVVNAEGVVEQRALVTDRAIGSQWLVSSGLKEGDQL 357 GVRQQGLLVPQQAVSRDGAGKPVAFVVNA+GVVEQRALVTDRAIG QWLVSSGLKEGDQL Sbjct: 301 GVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQL 360 Query: 358 VVDGQQRATAGAKVKVVPWSPKTTV 382 VVDGQQRA+AGAKVKVVPW+PK ++ Sbjct: 361 VVDGQQRASAGAKVKVVPWTPKASL 385 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 394 bits (1011), Expect = e-110, Method: Compositional matrix adjust. Identities = 207/362 (57%), Positives = 268/362 (74%), Gaps = 2/362 (0%) Query: 8 RPVAALAILSVLSACGKPPGGPPPAAGTPVLGVMTVRAQPVELHTELPGRTSPYQIADVR 67 R +L +++VL+AC + P P A V G + V+AQ L TELPGRT+ Y AD+R Sbjct: 20 RLALSLILVAVLAACSRSGDQPKPTAQAEV-GYVVVKAQRQALTTELPGRTTAYLAADIR 78 Query: 68 PQVNGIIKARNFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVKTARLKASRY 127 PQV GII+ R F+EGS VKAGQ LYQI+PASYQAAYDS+ A+L KAEA++ +++LKA RY Sbjct: 79 PQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERY 138 Query: 128 KELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYAKVDAPISGRIGKSSV 187 EL I AVS+QD DDA ++L + +ADV +A VQT+ INL Y K+ +PISGRI KS+V Sbjct: 139 AELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSAV 198 Query: 188 TPGALVTASQTTALTTIQQLDPIYVDVTQSSTNWLNLKEALANGELQKSG-NGAKVRLIL 246 TPGALVTASQTTALTT+QQ+DPIYVDVTQS+ + L L+ LA+G+++KSG N A+V+++L Sbjct: 199 TPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVL 258 Query: 247 ENGRTYAQEGTLQFSDVTVNQDTGAITLRAVFPNPKTDLLPGMYVRAVLQEGVRQQGLLV 306 E+G TY + G L+F+ ++V+ TG ITLRAV PNP LLPG YVRAVL+EGV LL Sbjct: 259 EDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLA 318 Query: 307 PQQAVSRDGAGKPVAFVVNAEGVVEQRALVTDRAIGSQWLVSSGLKEGDQLVVDGQQRAT 366 PQ+AVSRD G A V+ A+G V+QR + RA+G+ W VS GLKEGD+L+VDG Q+ Sbjct: 319 PQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVR 378 Query: 367 AG 368 G Sbjct: 379 TG 380 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 347 bits (891), Expect = 8e-97, Method: Compositional matrix adjust. Identities = 191/386 (49%), Positives = 262/386 (67%), Gaps = 8/386 (2%) Query: 6 LLRPVAALAILSVLSACGKPP---GGPPPAAGTPVLGVMTVRAQPVELHTELPGRTSPYQ 62 R +AI + L ACG+ P GGP P + + V+T+ Q V + ELPGR ++ Sbjct: 7 FTRLTMTVAIAAALVACGQKPAAQGGPMPPSE---VSVVTIAPQRVAITNELPGRVEAFR 63 Query: 63 IADVRPQVNGIIKARNFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVKTARL 122 +A VR +V GI++ R F+EGS+VK GQVL++I+PA Y+A+ +S+ A LA+A+A++ L Sbjct: 64 VAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTL 123 Query: 123 KASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYAKVDAPISGRI 182 A RYK LV + AVS+Q+YD+AVA+ +AEADV + +A VQT+ +NL YA V +PISGRI Sbjct: 124 TAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRI 183 Query: 183 GKSSVTPGALVTASQTTALTTIQQLDPIYVDVTQSSTNWLNLKEALANGELQKSG-NGAK 241 G++ VT GALV T L +QQLDP+YV+VTQSS + L L++A+A G+L+ G N A Sbjct: 184 GRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKAS 243 Query: 242 VRLILENGRTYAQEGTLQFSDVTVNQDTGAITLRAVFPNPKTDLLPGMYVRAVLQEGVRQ 301 V L LENG+ Y G L FSD++V+++TG+++LRA FPNPK LLPG YVRA +++ V + Sbjct: 244 VTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDE 303 Query: 302 QGLLVPQQAVSRDGAGKPVAFVVNAEGVVEQRALVTDRAIGSQWLVSSGLKEGDQLVVDG 361 Q LLVPQQAV RD G V VVNAE VE R + T A G+ W V GLKEGD+++V+G Sbjct: 304 QALLVPQQAVVRDAGGSTV-MVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEG 362 Query: 362 QQRATAGAKVKVVPWSPKTTVVSANG 387 Q+ GA VK V W+P A+G Sbjct: 363 LQKVRPGATVKPVQWNPAGAPAQASG 388 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 141 bits (355), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 106/337 (31%), Positives = 161/337 (47%), Gaps = 7/337 (2%) Query: 38 LGVMTVRAQPVELHTELPGRTSPYQIADVRPQVNGIIKARNFREGSDVKAGQVLYQINPA 97 +GV R Q V + G +P A VR +V+G + +++EG VKAG +L +I+P Sbjct: 34 VGVAKARLQDVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPR 93 Query: 98 SYQAAYDSSVAALAKAEASVKTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGS 157 S QAA + LA+ A + +ARL RY+ L+A +++ Q D VA + + E V + Sbjct: 94 SLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKA 153 Query: 158 ARANVQTSRINLDYAKVDAPISGRIGKSSVTPGALVTASQTTALTTIQQLDPIYVDVTQS 217 NV ++R+ L + +V APISGR+G G VT S T L I QL PI + Sbjct: 154 DEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIP 213 Query: 218 STNWLNLKEALANGELQKSGNGAKVRLILENGRTYAQEGTLQFSDVTVNQDTGAITLRAV 277 N + + L +SG + + +G L D V+ TG + L+A Sbjct: 214 EDNIPKVLQQL------QSGRKLPAQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQ 267 Query: 278 FPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAEGVVEQRALVT 337 FPN L P +V LQ Q ++P A+ R G V +VV + V R + T Sbjct: 268 FPNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFV-YVVKDDSSVTVRPVKT 326 Query: 338 DRAIGSQWLVSSGLKEGDQLVVDGQQRATAGAKVKVV 374 +++ G+ G+ +V+DG R GAKV+ V Sbjct: 327 GPVQDDLTVITDGVSAGETVVIDGIDRLREGAKVEAV 363 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 95/310 (30%), Positives = 154/310 (49%), Gaps = 12/310 (3%) Query: 66 VRPQVNGIIKARNFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVKTARLKAS 125 V+ +++G ++ F+EG DVK GQ+L QI+ + QA + A AK A++ ARL Sbjct: 79 VKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQ 138 Query: 126 RYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYAKVDAPISGRIGKS 185 RY L + A +QQ D A + + EA V + A V +++ L Y ++APISGR+G Sbjct: 139 RYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGAR 198 Query: 186 SVTPGALVTASQTTALTTIQQLDPIYVDVT--QSSTNWLNLKEALANGELQKSGNGAKVR 243 V PG +V A+ L I Q+DPI + T + + +N + LQ S + Sbjct: 199 LVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQ 258 Query: 244 LILENGRTYAQEGTLQFSDVTVNQDTGAITLRAVFPNPKTDLLPGMYVRAVLQEGVRQQG 303 +L G+ L D +N G + L+A F NP L PG YV A LQ Sbjct: 259 EVLATGK-------LVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNA 311 Query: 304 LLVPQQAVSRDGAGKPVAFVVNAEGVVEQRALVTDRAIGSQWLVSSG--LKEGDQLVVDG 361 L VP A+ R+ G V + ++ E + + +R ++++G ++ G ++V+DG Sbjct: 312 LTVPAAAIQRNQQGTYV-YAIDQEDQARMQPVKVERIQDGIAVLAAGNAVQAGQRVVIDG 370 Query: 362 QQRATAGAKV 371 Q + G+++ Sbjct: 371 QYKLKPGSRI 380 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,198 Number of extensions: 44835 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 12 Length of query: 394 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 307 Effective length of database: 1,207,925 Effective search space: 370832975 Effective search space used: 370832975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)