BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0063.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 274 1e-74 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 219 5e-58 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 216 4e-57 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 107 1e-24 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 103 2e-23 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 159/426 (37%), Positives = 244/426 (57%), Gaps = 2/426 (0%) Query: 6 SLDVRSHINGRKMSGYQWLLLILCFLIVATDGMDVAIMGFLAPAITKEWGISRAAFGVVM 65 ++ V + + +Q+ LL+LC L + DG DV MG++APAI +WG+S+A+ G V Sbjct: 11 TVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVF 70 Query: 66 SAAPIGLAVGALLVGPLSDRYGRKKLLMTAVAWFGACSLACAFAQDVVTLSALRFLTGLG 125 A G+ +G+L++ P+ DRYGR+ +L+ + +F C LA + L LRF+TG G Sbjct: 71 GAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFG 130 Query: 126 LGAAMPNATTLLSEYVPERSRSTLLAIMFTGFNLGSGLVGFLAAALLPDYGWRTVLMVGG 185 LG+ MPNA L+ E+ P SR T + ++ GF +G+ GF++AAL+P YGW V VGG Sbjct: 131 LGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWVGG 190 Query: 186 AIPL-LCLPFYLWLVPESARFMVVRHYPADRIARTLARVCGGHFAAGQTFSVSEPPVKGG 244 A+PL L L +WL PES +F+V+ P ++AR L ++ +T V + G Sbjct: 191 AVPLVLGLAMLVWL-PESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAEG 249 Query: 245 QPAKVLLSETYRKTTLSLWGTYFMGLLVIYLLSGWLPTLIKDAGLPIEKAASITALFQLG 304 P L + T+ LW FM L+ +Y LS WLPTLI+DAG A I Q+G Sbjct: 250 MPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQVG 309 Query: 305 GTVGALVVGFLMDRWSPSRAIACAYIAGAVFILLLASGSVQSGMFAAYVLLAGFCMSGAQ 364 G +G L +G + R+ +R + ++ + I L+ + + V++AGFC+ G Q Sbjct: 310 GVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGGQ 369 Query: 365 TGLNAFAPSCYPTVVRATGVSWMLGVGRFGSILGSFAGGVLLSMGWGFGAVIAILAVPAT 424 +NA A + YPT +R+TG+ W LG+GR GS++G GG L+++ W ++ AVPA Sbjct: 370 PAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVPAL 429 Query: 425 LAALII 430 ++AL I Sbjct: 430 ISALTI 435 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 219 bits (557), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 149/425 (35%), Positives = 225/425 (52%), Gaps = 5/425 (1%) Query: 5 QSLDVRSHINGRKMSGYQWLLLILCFLIVATDGMDVAIMGFLAPAITKEWGISRAAFGVV 64 ++LDV I+G + + + W+++ LC L++ DG D+ I G + P + KEW +S G + Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 Query: 65 MSAAPIGLAVGALLVGPLSDRYGRKKLLMTAVAWFGACSLACAFAQDVVTLSALRFLTGL 124 S A G+ GA + GPL+DR GRKK + + F + FA RFL GL Sbjct: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 Query: 125 GLGAAMPNATTLLSEYVPERSRSTLLAIMFTGFNLGSGLVGFLAAALLPDYGWRTVLMVG 184 G G MPNA L++EY P+R RSTL+AIMF+G++LG L L +LP++GW++ + Sbjct: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQS-MFFA 180 Query: 185 GAIPLLCLPFYLWLVPESARFMVVRHYPADRIARTLARVCGGHFAAGQTFSVSEPPVKG- 243 A+PL+ LP LW +PES F+ +R ++ L +V A T + + VKG Sbjct: 181 AAVPLVLLPVILWKLPESVGFL-LRQGRLEQARAMLTQVDPQARIATDT-QLVQADVKGA 238 Query: 244 GQPAKVLLSETYRKTTLSLWGTYFMGLLVIYLLSGWLPTLIKDAGLPIEKAASITALFQL 303 G P L + TL +W ++F LL++Y L WLP L+ AG + + S Sbjct: 239 GAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNF 298 Query: 304 GGTVGALVVGFLMDRWSPSRAIACAYIAGAVFILLLASGSVQSGMFAAYVLLAGFCMSGA 363 GG VGA+ G+L DR++ + + + G V I LL S ++ +++AG G Sbjct: 299 GGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLY-LLIIVAGATTIGT 357 Query: 364 QTGLNAFAPSCYPTVVRATGVSWMLGVGRFGSILGSFAGGVLLSMGWGFGAVIAILAVPA 423 Q L A YP +R+TG+ W GVGR G+I+G GG L++ + A+P Sbjct: 358 QILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPG 417 Query: 424 TLAAL 428 +AAL Sbjct: 418 LVAAL 422 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 216 bits (549), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 143/436 (32%), Positives = 231/436 (52%), Gaps = 14/436 (3%) Query: 5 QSLDVRSHINGRKMSGYQWLLLILCFLIVATDGMDVAIMGFLAPAITKEWGISRAAFGVV 64 Q++D+ ++ SG+Q L++ L L + DG D ++GF P++ KEWGI+R AF Sbjct: 10 QTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPA 69 Query: 65 MSAAPIGLAVGALLVGPLSDRYGRKKLLMTAVAWFGACSLACAFAQDVVTLSALRFLTGL 124 ++A +G+ +G+ G +DR+GR+ ++ +V FG + AF+ DV T++ LRF GL Sbjct: 70 VAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGL 129 Query: 125 GLGAAMPNATTLLSEYVPERSRSTLLAIMFTGFNLGSGLVGFLAAALLPDYGWRTVLMVG 184 G+G A+P++TT+ +E+ P R R+ + LG L G A A+LP YGWR + ++G Sbjct: 130 GIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIG 189 Query: 185 GAIPLLCLPFYLWLV-PESARFMVVRHYPADRIARTLARVCGGHFAAGQTFSVSEPPVKG 243 G +P + LW V PES R++ + D + R L R+ A E ++ Sbjct: 190 GLLP-VAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIET 248 Query: 244 GQPAKVLLSETYRKTTLSLWGTYFMGLLVIYLLSGWLPTLIKDAGL-PIEKAASITALFQ 302 L + + T++LW +FM LL +Y WLPT++ GL P A +TA + Sbjct: 249 RAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTA-YN 307 Query: 303 LGGTVGALVVGFLMDRWS---PSRAIACAYIAGAVFILLLASGSVQSGMFAAYVLLAGFC 359 GG GAL+ + R+ P A A A+F+ L S Q+ + + L G Sbjct: 308 FGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVAS-QTTLLVVGIGLHGLF 366 Query: 360 MSGAQTGLNAFAPSCYPTVVRATGVSWMLGVGRFGSILGSFAGGVLLSMGWGFGAVIAIL 419 ++ Q+ + A YPT VRATG + L GR G+IL +FAG ++++ G G +A+L Sbjct: 367 VNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQG-GAAGYLAML 425 Query: 420 AVPATLAALIIVFIQM 435 A A+++VF+ + Sbjct: 426 A-----GAMLMVFVAL 436 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 106/394 (26%), Positives = 178/394 (45%), Gaps = 10/394 (2%) Query: 8 DVRSHINGRKMSGYQWLLLILCFLIVATDGMDVAIMGFLAPAITKEWGISRAAFGVVMSA 67 V + + +Q+ L ++ L+ D M V +GF AP I K +G++ + Sbjct: 7 SVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTM 66 Query: 68 APIGLAVGALLVGPLSDRYGRKKLLMTAVAWFGACSLACAFAQDVVTLSALRFLTGLGLG 127 +G+ +GA + G L+DR GR+ +LM AV +A AFA D L ALR LTG+G+G Sbjct: 67 FFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVG 126 Query: 128 AAMPNATTLLSEYVPERSRSTLLAIMFTGFNLGSGLVGFLAAALLP--DYGWRTVLMVGG 185 +P T+++E++P R L ++ + + +G+ + LA + + WR + V G Sbjct: 127 GTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRVIFFVTG 186 Query: 186 --AIPLLCLPFYLWLVPESARFMVVRHYPADRIARTLARVCGGHFAAGQTFSVSEPPVKG 243 A+ + L FY VPES ++ R +D + L RV + + +P V Sbjct: 187 LPALVGVVLRFY---VPESPMYL-NRSGKSDAARQVLERVAKVN-GSSTPIPPLQPEVVE 241 Query: 244 GQPAKVLLSETYRKTTLSLWGTYFMGLLVIYLLSGWLPTLIKDAGLPIEKAASITALFQL 303 +P L S R+ +LSL+ + + + Y + +LP + G + L Sbjct: 242 RKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLAL 301 Query: 304 GGTVGALVVGFLMDRWSPSRAIACAYIAGAVFILLLASGSVQSGMFAAYVLLAGFCMSGA 363 G + + ++RW + + AV +L + G + LL F + G Sbjct: 302 VQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGK-DPMLVVGSTLLLSFSLLGT 360 Query: 364 QTGLNAFAPSCYPTVVRATGVSWMLGVGRFGSIL 397 L AF P YPT +RA+G+ V RFG + Sbjct: 361 WAALYAFTPEVYPTDLRASGMGTAGAVARFGGLF 394 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 93/370 (25%), Positives = 157/370 (42%), Gaps = 19/370 (5%) Query: 36 DGMDVAIMGFLAPAITKEWGISRAAFGVVMSAAPIGLAVGALLVGPLSDRYGRKKLLMTA 95 D + F+ P + W +S A G+++++ +G +GAL G +++RYGR Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 96 VAWFGACSLACAFAQDVVTLSALRFLTGLGLGAAMPNATTLLSEYVPERSRSTLLAIMFT 155 V S+ CA L RFL G+G+G +P A T ++E R + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 156 GFNLGSGLVGFLAAALLPDYGWRTVLMVGGAIPLLCLPFYLWLVPESARFMVVRHY--PA 213 F LG + A L+P +GW + +VGG IP + + + +PES R+++ R A Sbjct: 176 IFPLGLLAAAQVGAFLVPRFGWEYMFLVGG-IPGILVALLITRLPESPRWLIARGRLDDA 234 Query: 214 DRIARTLARVCGGHFAAGQTFSVSEPPVKGGQPA----------KVLLSETYRKTTLSLW 263 +++ RTL QT + A K L S YR T+ +W Sbjct: 235 EKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTIIVW 294 Query: 264 GTYFMGLLVIYLLSGWLPTLIKDA-GLPIEKAASITALFQLGGTVGALVVGFLMDRWSPS 322 + V ++ WLP+L K LP++++ + ++ + L L+DR Sbjct: 295 LLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRVGRR 354 Query: 323 RAIACAYIAGAVFILLLASGSVQSGMFAAYVLLAGFCMSGAQTG-LNAFAPSCYPTVVRA 381 R ++I + LL +S ++ + + + G T L + P YPT +RA Sbjct: 355 RWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVMGTTTVLLYLYTPEIYPTRMRA 414 Query: 382 TG----VSWM 387 G SW+ Sbjct: 415 IGTGLATSWL 424 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,417,885 Number of extensions: 61666 Number of successful extensions: 548 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 502 Number of HSP's successfully gapped: 31 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)