BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0048.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 869 0.0 gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 104 1e-23 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 90 4e-19 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 869 bits (2246), Expect = 0.0, Method: Compositional matrix adjust. Identities = 432/513 (84%), Positives = 451/513 (87%), Gaps = 36/513 (7%) Query: 1 MPEQTAAPVSPALPDDSIRFGLDDFRRMMEALPMCIILHDAQTKSILWANPSACQVLGFT 60 MPE TAAP DD IRFGLDDFRRMM+ALPMCIILHDAQTKSILWANPSACQVLGFT Sbjct: 1 MPEPTAAP-----EDDPIRFGLDDFRRMMDALPMCIILHDAQTKSILWANPSACQVLGFT 55 Query: 61 LEELLPLKAPDMSRQSHEYRREIGRKWLHDAVLHGSNMVEWCYRAKNGDEILSEAIATLV 120 LEELLPLKAPDMSRQSHEYRREIGRKWLHDA LHGSNMVEWCYRAKNG+EILSEAIATLV Sbjct: 56 LEELLPLKAPDMSRQSHEYRREIGRKWLHDATLHGSNMVEWCYRAKNGEEILSEAIATLV 115 Query: 121 PLTERDVIMVQFRDIAREEQIRREMKKLESRLKEFMQDSDEGVTVLRSDGGIEYLSDAGR 180 PLTERDVIMVQFR+IAREEQIRREMKKLESRLKEFMQDSDEGV VLR DGGIEYLSDAGR Sbjct: 116 PLTERDVIMVQFRNIAREEQIRREMKKLESRLKEFMQDSDEGVAVLRPDGGIEYLSDAGR 175 Query: 181 KLLGVRPGQNPPKNFVEQCLPESRDELLELLTLALPDAISFPLRYQIVRKDGLVRWHHAV 240 KLLGVRPGQNPPKNFVEQCLP+S++EL ELLTLALPDAISFPLRY+I RKDGLVRWHHAV Sbjct: 176 KLLGVRPGQNPPKNFVEQCLPDSQEELQELLTLALPDAISFPLRYRIARKDGLVRWHHAV 235 Query: 241 CRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQASLEYLARHNAMGEMATAIAHELSQP 300 CRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQA+LEYLARHNAMGEMATAIAHELSQP Sbjct: 236 CRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMATAIAHELSQP 295 Query: 301 LAAIRNFLEGSVLRIKQSEALREQAGQIVWGLESAGRQVDHAATIIKSVREYVVRLEQSE 360 LAAIRNFLEGSVLR+ SEALREQAGQI+WGLESAGRQVDHAA IIKSVREYVV+LEQSE Sbjct: 296 LAAIRNFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSE 355 Query: 361 GLIDLNAVLEEVRWFIEMKAAEGKVHLHIEPGTQPLVVSCEKVLIGQVILN--------- 411 GLIDLN VL EVRWFIEM+AAEG+V L ++ + LVVSCEKVLIGQVILN Sbjct: 356 GLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKVLIGQVILNLAFNAIEEM 415 Query: 412 --------------XKPPPQPL--------GIDAGHRERLFDGFFSSKVSGNGIGLALCK 449 + Q L GIDAGHRERLFDGFFSSKVSGNGIGLALCK Sbjct: 416 SHFPENQRRVCISLARKDQQALLTVADEGRGIDAGHRERLFDGFFSSKVSGNGIGLALCK 475 Query: 450 NIIARHRGDIWAQPGETRGTRFCFTLPLVERHN 482 NIIARHRGDIWAQP + RGTRFCFTLPLV+RHN Sbjct: 476 NIIARHRGDIWAQPAQVRGTRFCFTLPLVQRHN 508 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 107/391 (27%), Positives = 180/391 (46%), Gaps = 57/391 (14%) Query: 132 FRDIAREEQIRREMKKLESRLKEFMQDSDEGVTVLRS---DGGIEYLSDAGRKLLGVRP- 187 +RDI R + + + E+ + M++S VT +R+ +G I Y++ A K++G Sbjct: 289 WRDINRRTKAEKALLN-EAVFRAAMENSL--VTGMRARDLEGRITYVNPAFCKMVGFAAE 345 Query: 188 ---GQNPPKNFVEQCLPESRDELLELLTLALPDAISFPLRYQIVRKDGLVRWHHAVCRYI 244 G+ PP + PE+ DE E + L + R+DG R+ + Sbjct: 346 ELVGKIPPMPY---WAPEAMDEYQERFSNVLAGRATPQFETIFQRRDG-ERFPVLIFESP 401 Query: 245 EIENDLK--GHLLHFHDITDQVEAEEARRRDQASLEYLARHNAMGEMATAIAHELSQPLA 302 ++ D K G + DI+++ AE+ R + L+ AR +MGE+A+ +AHEL+QPLA Sbjct: 402 LVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARLASMGEIASTLAHELNQPLA 461 Query: 303 AIRNFLEGSVLRIKQSEALREQAGQIVWGLESAGRQVDHAATIIKSVREYVVRLEQSEGL 362 AI ++ G++ ++Q +A + LE Q A +I+SV ++V + E + Sbjct: 462 AISSYTTGALNLMQQGQA---DPKALQPALEKIHAQAQRAGHVIRSVHQFVKKREPARQR 518 Query: 363 IDLNAVLEEVRWFIEMKAAEGKVHLHIE-PGTQPLVVSCEKVLIGQVILN--------XK 413 + + +++ V IE++A + V L ++ P + P V+ +++L+ QVILN Sbjct: 519 VQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLG-DQILLEQVILNLTRNGIQAMA 577 Query: 414 PPPQPLGI----------------------DAGH------RERLFDGFFSSKVSGNGIGL 445 P I D GH RLF FFS+K G G+GL Sbjct: 578 AVPSARRILRVAAEVTRNEDGAESVTVSVTDQGHGIPDDVAARLFSPFFSTKAEGMGMGL 637 Query: 446 ALCKNIIARHRGDIWAQPGETRGTRFCFTLP 476 +C+ I H G + GT F F+LP Sbjct: 638 NICRTTIEFHGGTLTYTDNPAGGTIFRFSLP 668 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 85/340 (25%), Positives = 146/340 (42%), Gaps = 38/340 (11%) Query: 174 YLSDAGRKLLGVRPGQN--PPKNFVEQCLPESRDELLELLTLALPDAISFPLRYQIVRKD 231 Y SD ++ G+ PG+N + F P+ R E++E + A+ + + ++I R+D Sbjct: 187 YCSDELCRIYGLAPGRNCITYEEFAAIMHPDDRQEVVETVNAAVAMGGTIRVEHRICRQD 246 Query: 232 -GLVRWHHAVCRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQASLEYLARHNAMGEMA 290 G VR+ + + I + ++ DIT + + E A R Q +E ++R N +G++ Sbjct: 247 TGEVRYLSGIGKPIWRDGTFTEYVGTATDITVRRQNEYAIRAAQIDMERVSRANTVGQLT 306 Query: 291 TAIAHELSQPLAAIRNFLEGSVLRIKQSEALREQAGQIVWGLESAGRQVDHAATIIKSVR 350 +IAHE++QPL +I S+ + + E A GL + A II +R Sbjct: 307 ASIAHEINQPLMSIVANAGASLRWLDRPVPDLEHART---GLRDIIAEGQRAGGIISGLR 363 Query: 351 EYVVRLEQSEGLIDLNAVLEEVRWFIEMKAAEGKVHLHIEPGTQPLVVSCEKVLIGQVIL 410 + ++L+AV+ + + +V L + V ++V + QV+L Sbjct: 364 DLTRNAAPVCERVNLHAVIRHILTLSRSELERREVSLLLSLTPADAFVLGDQVQLQQVML 423 Query: 411 N--------------------------------XKPPPQPLGIDAGHRERLFDGFFSSKV 438 N K GI R+FD F+++K Sbjct: 424 NLVVNAIEAMSSMPEHARVLSITASSCDEQRIEVKVQDSGEGIAPEAMRRVFDAFYTTKD 483 Query: 439 SGNGIGLALCKNIIARHRGDIWAQPGETRGTRFCFTLPLV 478 SG G+GLA+C++II HRG + + G F F LP V Sbjct: 484 SGMGMGLAICRSIIENHRGRLRVAAAQPHGALFSFDLPRV 523 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,542,069 Number of extensions: 64202 Number of successful extensions: 280 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 4 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)