BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0041.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 422 e-119 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 151 1e-37 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 145 4e-36 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 66 5e-12 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 55 6e-09 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 422 bits (1086), Expect = e-119, Method: Compositional matrix adjust. Identities = 203/217 (93%), Positives = 209/217 (96%) Query: 1 MKLIVDTVTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 MKLIVDT+TDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 61 YTIAVKREPASRGGSAALHDAVKPGMELEVGTPRNLFALDAGAQEHLLFGAGIGVTPLLA 120 YTIAVKRE ASRGGSAALHD VK GMELEVGTPRNLFALDA A EHLLFGAGIGVTPLLA Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 121 MAYRLWAEQKPFTLHYFVRAREHAAFADLLENAPFAAQVRFHYAVMPAQMDHYLTACLEA 180 MAYRLWAE+K FTLHYFVRAREHAAFADLLENAPFAA VRFHYAVMPAQM+HYLT CLEA Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 181 GSKEAHVYTCGPAPFMDAVVKCAAASRSEDAIHLEHF 217 GSKEAHVYTCGPAPFMDAVVKCA+A+RSE+AIHLEHF Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASATRSEEAIHLEHF 217 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 151 bits (381), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 106/247 (42%), Positives = 131/247 (53%), Gaps = 15/247 (6%) Query: 10 DETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCGYTIAVKREP 69 +E DI + LV DGA LPA+ GAHIDV P G++RQYSLC ++ Y I V R+ Sbjct: 13 EEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHRYQIGVLRDV 72 Query: 70 ASRGGSAALHDAVKPGMELEVGTPRNLFALDAGAQEHLLFGAGIGVTPLLAMAYRLWAEQ 129 SRGGS A+H+A+K G L + PRN FAL AQ LL GIGVTP+L MA L A Sbjct: 73 NSRGGSVAVHEALKLGDILTISAPRNQFAL-VPAQHSLLLAGGIGVTPILCMAEALAAAG 131 Query: 130 KPFTLHYFVRAREHAAFADLLENAPFAAQVRFHYAVMPAQMDHYLTACLEAGSKEAHVYT 189 F +HY R+ AF + + + FAA+V HY A L A L H+Y Sbjct: 132 ASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLAQADPATHLYV 191 Query: 190 CGPAPFMDAVVKCAAASR-SEDAIHLEHF-AAPVAPAASAQGAGGGDGAFEV----XGAV 243 CGPA F+ VV A A E +H E+F AAPV G+GAFEV G V Sbjct: 192 CGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPV--------KADGNGAFEVKLASSGQV 243 Query: 244 PVLWPAR 250 + P R Sbjct: 244 YTIPPER 250 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 145 bits (367), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 92/218 (42%), Positives = 119/218 (54%), Gaps = 3/218 (1%) Query: 1 MKLIVDTVTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 + L V V DIR LV DGA LP + PGAH+ + G++R YSLC + R Sbjct: 16 LMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDC 75 Query: 61 YTIAVKREPASRGGSAALHDAVKPGMELEVGTPRNLFALDAGAQEHLLFGAGIGVTPLLA 120 Y IAVKREP SRGGSA LH+ V+ G + P N F L A + LL AGIG+TPL A Sbjct: 76 YLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHA 135 Query: 121 MAYRLWAEQKPFTLHYFVRAREHAAFADLLENAPFAAQVRFHYAVMPAQMDHYLTACLEA 180 MA L + + LHYF R+ HAAF E A + + H + A+ +H + A L A Sbjct: 136 MAAELATQGRRHRLHYFARSLAHAAF--FHELAAGSQGMSLHLGLDGAETEHAIAAALAA 193 Query: 181 GSKEAHVYTCGPAPFMDAVVKCAA-ASRSEDAIHLEHF 217 + + +Y CGP+PF+DA + A A E IH E F Sbjct: 194 APRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERF 231 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 70/227 (30%), Positives = 95/227 (41%), Gaps = 31/227 (13%) Query: 2 KLIVDTVTDETGDIRSFRLVRADGAPLPAYEPGAHID---VTGPTGIMRQYSLCGPLEDR 58 IV E+ +I SF V DG L + PG +I V RQYSL L Sbjct: 157 NFIVSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQA 216 Query: 59 CG---YTIAVKREPASRGGSAALHDAVKPGMELEVGTPRNLFALDAGAQEHLLFGAGIGV 115 Y I+VKREP + S LHD + G +E+ P F L A + L G+G+ Sbjct: 217 GQPPQYRISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGI 275 Query: 116 TPLLAMAYRLWAEQKPFTLHYFVRAREHA---AFADLLENAPFAA-----QVRFHYAVMP 167 TP+++M +P +F+ A H AF LE AA Q+RF Y P Sbjct: 276 TPMMSMLAAALPSGRPI---HFIHAARHGGVHAFRRQLEE--LAARHPQLQLRFCYE-QP 329 Query: 168 AQMDH--YLTACLE--------AGSKEAHVYTCGPAPFMDAVVKCAA 204 Q D + T +E G+ + Y GP FM A+ + A Sbjct: 330 RQGDAAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLA 376 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 55.5 bits (132), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 74/268 (27%), Positives = 115/268 (42%), Gaps = 31/268 (11%) Query: 3 LIVDTVTDETGD--IRSFRLVRADGAPLPAYEPGAHIDVTGPTG---IMRQYSLCGPLED 57 L + V ET D + SF V A+ +Y+ G H+ + + R YS+C +++ Sbjct: 7 LTISDVKQETRDTIVVSFA-VPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSICSAVQE 65 Query: 58 RCGYTIAVKREPASRGGSAALHDAVKPGMELEVGTPRNLFA--LDAGAQEHLL-FGAGIG 114 R +A+KR P + A +++ PG ++V P F L+AG ++H L F AG G Sbjct: 66 R-QLRVAIKRAPGGLFSNWA-NESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSG 123 Query: 115 VTPLLA-MAYRLWAE-QKPFTLHYFVRAREHAAFADLLENAPFAAQVRFHYA-VMP---- 167 +TP+++ + L +E FTL Y RA F + L + A RF+ VM Sbjct: 124 ITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDAYLERFNVVYVMSREQQ 183 Query: 168 -----------AQMDHYLTACLEAGSKEAHVYTCGPAPFMDAV-VKCAAASRSEDAIHLE 215 A+ D + + ++ +A + CGP + AV A + I E Sbjct: 184 DVELFNGRIDRAKCDAFFASWIDLKDVDA-AFLCGPEEMVQAVAASLQAHGLPKTQIKTE 242 Query: 216 HFAAPVAPAASAQGAGGGDGAFEVXGAV 243 FAA P A A G EV V Sbjct: 243 LFAASTPPRAHAARTVVGKQECEVTVIV 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.135 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 944,279 Number of extensions: 36779 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 5 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)