BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0032.p (592 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 453 e-128 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 275 9e-75 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 244 1e-65 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 239 4e-64 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 231 1e-61 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 453 bits (1165), Expect = e-128, Method: Compositional matrix adjust. Identities = 248/459 (54%), Positives = 314/459 (68%), Gaps = 1/459 (0%) Query: 134 VIEPATGAALAATGIGNADDIDRAVTSAATAQPAWAAITFSERARILREAARLMQLHAEE 193 V+EPATG L G+ + DD+ +V A AQ AW A+ ERA I R AA L H +E Sbjct: 30 VLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPRERADIFRRAAGLFHQHFDE 89 Query: 194 FNRWNVRECGSTTAKAQWELAQCCEQLHMAAALPMQPDGLLFPSSLPGRSNQWRQIPIGV 253 R RE G K + E+ + H+AA +P+Q G L PS+ PGR + R+ P+GV Sbjct: 90 LARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLPST-PGRLSIARRAPLGV 148 Query: 254 VGVISPWNFPLLLSLRSVAPALALGNAVVLKPDTQSAVCGGLLLARLFELAGLPQGVLHV 313 +GVISP+NFPL+L+LR+VAPALA GNAVV+KPD ++ V GG +LAR+FE AGLP G+LHV Sbjct: 149 IGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLARIFEQAGLPAGLLHV 208 Query: 314 IPGGPAAGQALVAHAQVGMIAFTGSTAVGRAIGQHCGQSLKKVALELGGNNAQVVLADAD 373 +PGG G+ALV V MIAFTGS AVGR IG+ G+ LKK++LELGG NA V+L DAD Sbjct: 209 LPGGAETGEALVTAPGVPMIAFTGSPAVGRRIGELAGRHLKKLSLELGGANALVILEDAD 268 Query: 374 LDAAASNGAWASFLHQGQICMQAGRHLVHRSVAALYAEKLAARAKALHVGDPYRGQVHLG 433 LD AAS+ AW ++LHQGQICM A R LVH S+ ++LA +A+ L VGD GQV LG Sbjct: 269 LDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLADKARHLPVGDGASGQVALG 328 Query: 434 PLINARQRDRLHGLVTQSIAAGATLLAGGHYDELFYAPTVLAGVTPEMPVYHEELFGPVA 493 P+I+ RQ R+H LV +S+AAGA L+AGG Y++LFY PTVL GV P M V+ EE+FGPVA Sbjct: 329 PMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLFYQPTVLGGVRPGMRVFEEEIFGPVA 388 Query: 494 PITVFDDERQAIALVNGSPYGLAAAVHSRDVARATALGRQLRCGMVHINDQTVNNEFQVP 553 + FD++ AIAL N GLAA V S V RA A+ +LR GMVH+NDQTVN+E P Sbjct: 389 SVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMAVAARLRQGMVHVNDQTVNDECVNP 448 Query: 554 FGGMGDSGNASRFGGPASVHEFTQSQWISIMDQPIQYPF 592 FGG G +GN + GGPA E+TQ +W +I Q + YPF Sbjct: 449 FGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQAMPYPF 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 275 bits (702), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 171/422 (40%), Positives = 244/422 (57%), Gaps = 6/422 (1%) Query: 175 ERARILREAARLMQLHAEEFNRWNVRECGSTTAKAQWELAQCCEQLHMAAALPMQPDGLL 234 +R +L AA +Q A++F + E G++ A + + + L AAA+ Q G + Sbjct: 64 QRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEV 123 Query: 235 FPSSLPGRSNQWRQIPIGVVGVISPWNFPLLLSLRSVAPALALGNAVVLKPDTQSAVCGG 294 PS +PG + P GVV I+PWN P++L +R++A LA GN VV K G Sbjct: 124 IPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHG 183 Query: 295 LLLARLFELAGLPQGVLHVIPGGP----AAGQALVAHAQVGMIAFTGSTAVGRAIGQHCG 350 L++A L E AGLP GV++ + P A +A++A V + FTGST VGR I C Sbjct: 184 LIIAALQE-AGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQCA 242 Query: 351 QSLKKVALELGGNNAQVVLADADLDAAASNGAWASFLHQGQICMQAGRHLVHRSVAALYA 410 + LK LELGG +VL DADLDAA A+ +F + GQICM R +V SVA + Sbjct: 243 RHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFV 302 Query: 411 EKLAARAKALHVGDPYRGQVHLGPLINARQRDRLHGLVTQSIAAGATLLAGGHYDELFYA 470 +LAA+A +L +GDP +G V LG +++ +R + L+ ++A GATL+ GG + Sbjct: 303 RRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTLMP 362 Query: 471 PTVLAGVTPEMPVYHEELFGPVAPITVFDDERQAIALVNGSPYGLAAAVHSRDVARATAL 530 T+L V+ M +Y EE FGPV PI + AIA N + YGL++A+ SRDVARA AL Sbjct: 363 ATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARALAL 422 Query: 531 GRQLRCGMVHINDQTVNNEFQVPFGGMGDSGNASRFGGPASVHEFTQSQWISIMDQPIQY 590 R++ G+ HIN TV++E Q+PFGG+ SG FGG A + EFT+ +W+++ P Y Sbjct: 423 SRRIESGICHINGPTVHDEAQMPFGGVKSSG-FGHFGGKAGIAEFTELRWVTVQTTPRHY 481 Query: 591 PF 592 PF Sbjct: 482 PF 483 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 244 bits (623), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 160/429 (37%), Positives = 217/429 (50%), Gaps = 6/429 (1%) Query: 137 PATGAALAATGIGNADDIDRAVTSAATAQPAWAAITFSERARILREAARLMQLHAEEFNR 196 PA G L + A + RA+ +A A PAW T ER+ ILR LM + ++ Sbjct: 34 PANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELMLANTDDLAL 93 Query: 197 WNVRECGSTTAKAQWELAQCCEQLHMAAALPMQPDGLLFPSSLPGRSNQWRQIPIGVVGV 256 E G A+A+ E+ + A + G PS P + IGV Sbjct: 94 IMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAA 153 Query: 257 ISPWNFPLLLSLRSVAPALALGNAVVLKPDTQSAVCGGLLLARLFELAGLPQGVLHVIPG 316 I+PWNFP + R PALA G +VLKP ++ L LA L E AG+P GV V+ G Sbjct: 154 ITPWNFPAAMITRKAGPALAAGCPMVLKP-AEATPFSALALAVLAERAGIPAGVFSVVTG 212 Query: 317 GPAA-GQALVAHAQVGMIAFTGSTAVGRAIGQHCGQSLKKVALELGGNNAQVVLADADLD 375 P G + ++ V I+FTGST VG+ + Q S+KK++LELGGN +V DADLD Sbjct: 213 TPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLD 272 Query: 376 AAASNGAWASFLHQGQICMQAGRHLVHRSVAALYAEKLAARAKALHVGDPYRGQVHLGPL 435 AA + + + GQ C+ A R V V +A KL K VG V GPL Sbjct: 273 AAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPL 332 Query: 436 INARQRDRLHGLVTQSIAAGATLLAGGHYDEL---FYAPTVLAGVTPEMPVYHEELFGPV 492 IN + ++ V ++A GA +L GG L F+ PTVLA VTP M V EE FGP+ Sbjct: 333 INEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAMQVAREETFGPM 392 Query: 493 APITVFDDERQAIALVNGSPYGLAAAVHSRDVARATALGRQLRCGMVHINDQTVNNEFQV 552 AP+ F + + +AL N + +GLA+ +SRD+ R + L GMV IN ++NE Sbjct: 393 APLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEV-A 451 Query: 553 PFGGMGDSG 561 PFGG+ SG Sbjct: 452 PFGGVKQSG 460 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 239 bits (610), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 152/472 (32%), Positives = 244/472 (51%), Gaps = 8/472 (1%) Query: 119 LIAGCLXLTAGGHT-DVIEPATGAALAATGIGNADDIDRAVTSAATAQPAWAAITFSERA 177 I G +A G T DVI PAT + + D+DRA+ +A+ W ++ ER+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 178 RILREAARLMQLHAEEFNRWNVRECGSTTAKAQWELAQCCEQLHMAAALPMQPDGLLFPS 237 +I+R AA L++ AEE R E G A+A+ E + + A + G L P+ Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 238 SLPGRSNQWRQIPIGVVGVISPWNFPLLLSLRSVAPALALGNAVVLKPDTQSAVCGGLLL 297 +PG + P+G V +PWNFPL +R ++ ALA G ++++K ++ L+ Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 298 ARLFELAGLPQGVLHVIPGGPAA-GQALVAHAQVGMIAFTGSTAVGRAIGQHCGQSLKKV 356 R + AG+P GV++++ G PA + L+ H + I+FTGST VG+ + GQ +K+ Sbjct: 188 -RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRA 246 Query: 357 ALELGGNNAQVVLADADLDAAASNGAWASFLHQGQICMQAGRHLVHRSVAALYAEKLAAR 416 +ELGG+ +V DAD+D AA A A F + GQ+C+ R LV + V + +K Sbjct: 247 TMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTY 306 Query: 417 AKALHVGDPYRGQVHLGPLINARQRDRLHGLVTQSIAAGATLLAGGH---YDELFYAPTV 473 A+ L VG+ + +GP+ N R+ L L+ +++ GA L GG F+ PTV Sbjct: 307 AEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTV 366 Query: 474 LAGVTPEMPVYHEELFGPVAPITVFDDERQAIALVNGSPYGLAAAVHSRDVARATALGRQ 533 L + + +EE FGP+A I F+ + I N PYGLAA ++ AL + Sbjct: 367 LTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAAR 426 Query: 534 LRCGMVHINDQTVNNEFQVPFGGMGDSGNASRFGGPASVHEFTQSQWISIMD 585 + GM+ IN + ++PFGG+ DSG S GG ++ + ++ ++ +D Sbjct: 427 VESGMLTINHLGLALP-ELPFGGIKDSGYGSE-GGTEAIEAYINTKLVTQLD 476 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 231 bits (589), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 172/467 (36%), Positives = 245/467 (52%), Gaps = 20/467 (4%) Query: 129 GGHTDVIEPATGAALAATGIGNADDIDRAVTSAATAQPAWAAITFSERARILREAARLMQ 188 G DV +PATG A+ G A D AV +A A AW A +RA I++ L+ Sbjct: 28 GRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQRAGIIKRWNDLLL 87 Query: 189 LHAEEFNRWNVRECGSTTAKAQWELAQCCEQLHMAAALPMQPDGLLFPSSLPGRSNQWRQ 248 H ++ R RE G A+A+ E+A + + +G + P+ + GR + Sbjct: 88 AHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALK 147 Query: 249 IPIGVVGVISPWNFPLLLSLRSVAPALALGNAVVLKPDTQSAVCGGLLLARLFELAGLPQ 308 P+GVV I+PWNFP + R +APALA G VV KP + + L L RL + AG+P Sbjct: 148 EPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTS-LALVRLAQEAGVPV 206 Query: 309 GVLHVIPGG----PAAGQALVAHAQVGMIAFTGSTAVGRAIGQHCGQSLKKVALELGGNN 364 GV++++ P +A +V I+FTGSTAVG+ + +H +LKK++LELGGN Sbjct: 207 GVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLKKLSLELGGNA 266 Query: 365 AQVVLADADLDAAASNGAWASFLHQGQICMQAGRHLVHRSVAALYAEKLAARAKALHVGD 424 +V DAD+DAA A F + GQ C+ R V V + +KL AR AL VG Sbjct: 267 PFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLGARVAALKVGP 326 Query: 425 PYRGQVHLGPLINARQRDRLHGLVTQSIAAGATLLAGGHY-------DELFYAPTVLAGV 477 +GP+INAR ++ V +IA GA ++ GG + +YAPTVLA V Sbjct: 327 ATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDNYYAPTVLADV 386 Query: 478 TPEMPVYHEELFGPVAPITVFDDERQAIALVNGSPYGLAAAVHSRDVARATALGRQLRCG 537 T M EE FGPVAPIT F E + IA N +P+GLAA +S DV R + L G Sbjct: 387 TGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRIHRVTDALESG 446 Query: 538 MVHINDQTVNNEFQVPFGGMGDSGNASRFGGPASVH---EFTQSQWI 581 +V +N+ + E PFGG+ +SG +G SVH ++ ++++ Sbjct: 447 IVGVNEGALAAE-AAPFGGVKESG----YGREGSVHGLDDYLHTKYV 488 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,629,962 Number of extensions: 63822 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 21 Length of query: 592 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 501 Effective length of database: 1,188,985 Effective search space: 595681485 Effective search space used: 595681485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)