BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0048.p (104 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314050|ref|YP_003778142.1| hypothetical protein Hsero_4773... 83 6e-18 >gi|300314050|ref|YP_003778142.1| hypothetical protein Hsero_4773 [Herbaspirillum seropedicae SmR1] Length = 129 Score = 82.8 bits (203), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 50/130 (38%), Positives = 63/130 (48%), Gaps = 27/130 (20%) Query: 1 MTISFTDPSLPGQLQQGQVQTRRLQGGVRIRVTSGLVWLTIAGCRDDIWLQPGRCFDYHG 60 M+I F + +L GQ L G IRV GLVWLT+ D+WL GR FD G Sbjct: 1 MSIPFATETTTIRLAAGQTHALVLHGAACIRVHQGLVWLTVEQGGADVWLSAGRSFDIQG 60 Query: 61 RGDAIFEAVNGAAEFTVTPLPPKG--------------------------CGDDPIRIAK 94 RG A+ EA +GAAEF + P G CG+ +R+++ Sbjct: 61 RGLAVLEAAHGAAEFDLQMQPGWGRRLLRWLRGPGRPPVASSTSSATPPSCGET-VRVSR 119 Query: 95 LLPHGLIRWF 104 LLPHGLIRWF Sbjct: 120 LLPHGLIRWF 129 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.145 0.485 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 405,464 Number of extensions: 16081 Number of successful extensions: 47 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 46 Number of HSP's successfully gapped: 1 Length of query: 104 Length of database: 1,619,870 Length adjustment: 71 Effective length of query: 33 Effective length of database: 1,283,685 Effective search space: 42361605 Effective search space used: 42361605 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 123 (52.0 bits)