BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0045.p (472 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrog... 859 0.0 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 161 1e-40 gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrog... 152 8e-38 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 149 6e-37 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 147 2e-36 >gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 472 Score = 859 bits (2220), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/472 (92%), Positives = 454/472 (96%) Query: 1 MTDTTAAELQQLFTAQQQAFAADSDPSHAVRMDRLARLQALMDEHEAALIAAIDADFGGR 60 MTDTTAA LQQLFTAQ+QAFAADSDPSHA+RMDRLARLQALM+EHE ALIAAIDADFGGR Sbjct: 1 MTDTTAARLQQLFTAQRQAFAADSDPSHALRMDRLARLQALMEEHETALIAAIDADFGGR 60 Query: 61 SQHETRMAELFVLRAGIRHAQSHLKRWMRPQRVATALHFLPGSNRLLAQPLGVVGIISPW 120 S HETRMAELFVLRAG+RHAQSHLKRWMRPQRVATALHFLPGSNRLLAQPLGVVG+ISPW Sbjct: 61 SPHETRMAELFVLRAGLRHAQSHLKRWMRPQRVATALHFLPGSNRLLAQPLGVVGVISPW 120 Query: 121 NYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEMAVVTGDASIGQ 180 NYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEMAVVTGDA+IGQ Sbjct: 121 NYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEMAVVTGDAAIGQ 180 Query: 181 AFASLPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDASCRMTSSLQRIAF 240 AFA+LPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILD SCR+ SSLQRIAF Sbjct: 181 AFAALPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCRLHSSLQRIAF 240 Query: 241 GKLLNAGQTCVAPDYLLVPRGQAASVAQQLAEAMGRLYPRLHDNPDYTAIISLRHRARLQ 300 GKLLNAGQTCVAPDYLLVPRGQAA VAQ+LA+AMGRLYPRLHDNPDYTAIIS RHRARLQ Sbjct: 241 GKLLNAGQTCVAPDYLLVPRGQAAGVAQELAQAMGRLYPRLHDNPDYTAIISPRHRARLQ 300 Query: 301 AMVEEARTAGAQVIEVNPAGEQLGQSRKLAPTLILGATASMRVLQEEIFGPVLPILEYET 360 A+VEEAR GAQVIEVNPAGE+LG SRKLAPTLILGAT SMRV+QEEIFGPVLPILEY+ Sbjct: 301 ALVEEARGGGAQVIEVNPAGEELGHSRKLAPTLILGATPSMRVMQEEIFGPVLPILEYDQ 360 Query: 361 PEQAIAHVRQGPRPLALYWFGEDAARREQVLRQTHAGGVTINDCLWHLVQEEQPFGGVGE 420 E AIA VRQGPRPLALYWFGEDAARREQ+LRQTHAGGVTINDCLWHLVQEEQPFGGVGE Sbjct: 361 AEDAIAQVRQGPRPLALYWFGEDAARREQMLRQTHAGGVTINDCLWHLVQEEQPFGGVGE 420 Query: 421 SGMGAYHGRWGFDTFSKRKPVFRQARWNGGTLLHPPYGKKFEWVMELIRRLS 472 SGMGAYHGRWGFDTFSKRKPVFRQAR+NGGTLLHPPYGK FE V+ LIRRLS Sbjct: 421 SGMGAYHGRWGFDTFSKRKPVFRQARFNGGTLLHPPYGKTFERVLGLIRRLS 472 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 161 bits (407), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 139/448 (31%), Positives = 215/448 (47%), Gaps = 26/448 (5%) Query: 5 TAAELQQLFTAQQQAFAADSDPSHAVRMDRLARLQALMDEHEAALIAAIDADFGGRSQHE 64 AA++ Q A ++A A + S R L R+ AL+ E A+ + G + Sbjct: 51 NAADVDQAVQAARRAIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISY- 109 Query: 65 TRMAELFVLRAGIRHAQSHLKRW--MRPQRVATALHFLPGSNRLLAQPLGVVGIISPWNY 122 R A + + +R A+ +L + +RP+ G+ ++ +P+GV +I+PWN+ Sbjct: 110 ARTAHVPLAAEHLRVARDNLADYPFIRPR----------GTTAIVREPIGVCALITPWNW 159 Query: 123 PLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEM-AVVTGD-ASIGQ 180 P+ ALAAG V+LKPSEL+P + L AE+V P + +V G A +G Sbjct: 160 PIYQITAKVGPALAAGCTVVLKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGA 219 Query: 181 AFASLP-FDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDASCRMTSSLQRIA 239 A ++ P D + TGST+ G +AQ AA + V ELGGKSP ++ + ++ Sbjct: 220 ALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGV 279 Query: 240 FGKLLNAGQTCVAPDYLLVPRGQAASVAQQLAEAMGRLYPRLHD-NPDYTAIISLRHRA- 297 L N GQ+C AP L+VPR A V + + ++ ++ D N T L +RA Sbjct: 280 AAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQM--KVGDPNDAATTHGPLANRAQ 337 Query: 298 --RLQAMVEEARTAGAQVIEVNPA-GEQLGQSRKLAPTLILGATASMRVLQEEIFGPVLP 354 R+Q M+E GA+++ P + L Q PT+ M + QEEIFGPVL Sbjct: 338 FLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLA 397 Query: 355 ILEYETPEQAIAHVRQGPRPLALYWFGEDAARREQVLRQTHAGGVTINDCLWHLVQEEQP 414 IL Y+T E+AIA L + G D + V + +G V +N W + P Sbjct: 398 ILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVHLNYPAW---DPQAP 454 Query: 415 FGGVGESGMGAYHGRWGFDTFSKRKPVF 442 FGG +SG G +G G + + + K + Sbjct: 455 FGGYKQSGNGREYGVEGMEEYMEVKAIL 482 >gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 476 Score = 152 bits (383), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 114/341 (33%), Positives = 161/341 (47%), Gaps = 15/341 (4%) Query: 104 NRLLAQPLGVVGIISPWNYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARY 163 + L +P GV+ I+PWN+PL +++ + AL AGN V+LKPSE T LA +VA Sbjct: 136 DELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYTSVCGLELARVVADA 195 Query: 164 YAPEEMAVVTGDASIGQAFA-SLPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSP 222 P + V+GD S+G + + FTGS G +I AA NLTP T+ELGG Sbjct: 196 LPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAARNLTPTTMELGGNDA 255 Query: 223 AILDASCRMTSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAASVAQQL-----AEAMGR- 276 AI+ + + +G LN GQTC L VP + +L A MG Sbjct: 256 AIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVDELKAIIAALPMGDG 315 Query: 277 LYPRLHDNPDYTAIISLRHRARLQAMVEEARTAGAQVIEVNPAGEQLGQSRKLAPTLILG 336 L P + P + + ++ A+VE AR G ++ + G L TL+ G Sbjct: 316 LQPGIAMGP----VQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGNFYPL--TLVTG 369 Query: 337 ATASMRVLQEEIFGPVLPILEYETPEQAIAHVRQGPRPLALYWFGEDAARREQVLRQTHA 396 R++ EE FGPVLPI+ Y EQAIA+ + L + D+ R QV + A Sbjct: 370 LRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRAAQVATRLQA 429 Query: 397 GGVTINDCLWHLVQEEQPFGGVGESGMGAYHGRWGFDTFSK 437 G V +N +V PFGG SG G G+ G ++ Sbjct: 430 GTVWVNQH--GMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQ 468 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 149 bits (375), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 118/372 (31%), Positives = 188/372 (50%), Gaps = 15/372 (4%) Query: 74 RAGIRHAQSHLKRWMRPQRVATALHFLPG---SNRL--LAQPLGVVGIISPWNYPLQLAL 128 R I +A S ++ W + T +P SNR+ + + +GV I+PWN+P + Sbjct: 107 RGEIGYAASFIE-WFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMIT 165 Query: 129 GPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEM-AVVTGD-ASIGQAFASLP 186 A ALAAG ++LKP+E TP + LA L R P + +VVTG IG S P Sbjct: 166 RKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNP 225 Query: 187 F-DHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDASCRMTSSLQRIAFGKLLN 245 + FTGST VG+ + Q +A+++ ++LELGG +P I+ + ++++ K N Sbjct: 226 IVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRN 285 Query: 246 AGQTCVAPDYLLVPRGQAASVAQQLAEAMGRLYPRLHDNPDYTA--IISLRHRARLQAMV 303 AGQTCV + + V G + A +L EA+ + T +I+ + +++ V Sbjct: 286 AGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHV 345 Query: 304 EEARTAGAQVIEVNPAGEQLGQSRKLAPTLILGATASMRVLQEEIFGPVLPILEYETPEQ 363 +A GA+V+ + LG S PT++ T +M+V +EE FGP+ P+ ++T E+ Sbjct: 346 ADAVAKGARVL-LGGKRHALGHSF-FEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEE 403 Query: 364 AIAHVRQGPRPLALYWFGEDAARREQVLRQTHAGGVTINDCLWHLVQEEQPFGGVGESGM 423 +A LA Y++ D R +V +G V IN L + E PFGGV +SG+ Sbjct: 404 VLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGL--ISNEVAPFGGVKQSGL 461 Query: 424 GAYHGRWGFDTF 435 G +G D + Sbjct: 462 GREGSHYGIDDY 473 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 147 bits (371), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 115/347 (33%), Positives = 168/347 (48%), Gaps = 23/347 (6%) Query: 109 QPLGVVGIISPWNYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEE 168 +PLGVVG I PWN+P+ +A+ ALAAGN V+LKP+E TP +L EL+A P Sbjct: 153 EPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIADLIPPGV 212 Query: 169 MAVVTG-DASIGQAFASLP-FDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILD 226 + +V G G+ AS + FTG T GR I Q A+ NL PVTLELGGKSP I Sbjct: 213 VNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFF 272 Query: 227 ASC------RMTSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAASVAQQLAEAMGRLYPR 280 A +L+ A LN G+ C P +LV Q + + + A+ R+ Sbjct: 273 ADVLDKDDDFFDKALEGFAMFA-LNQGEVCTCPSRVLV---QESIYERFIERALKRVAAI 328 Query: 281 LHDNP-DYTAII----SLRHRARLQAMVEEARTAGAQVIE---VNPAGEQLGQSRKLAPT 332 NP D + +I S ++ + ++ + GA+V+ G L + PT Sbjct: 329 KQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPT 388 Query: 333 LILGATASMRVLQEEIFGPVLPILEYETPEQAIAHVRQGPRPLALYWFGEDAARREQVLR 392 + G MR+ QEEIFGPV+ + ++ E+A+A L + D R ++ R Sbjct: 389 VFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGR 447 Query: 393 QTHAGGVTINDCLWHLVQEEQPFGGVGESGMGAYHGRWGFDTFSKRK 439 + AG V N +HL FGG +SG+G + + D + + K Sbjct: 448 EIQAGRVWTN--CYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTK 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,487,304 Number of extensions: 64649 Number of successful extensions: 299 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 20 Length of query: 472 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 383 Effective length of database: 1,198,455 Effective search space: 459008265 Effective search space used: 459008265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)