BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0044.p (543 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspi... 1017 0.0 gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspi... 335 8e-93 >gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 1017 bits (2630), Expect = 0.0, Method: Compositional matrix adjust. Identities = 506/543 (93%), Positives = 521/543 (95%) Query: 1 VLFDYVIAGGGSAGATLAARLSEDPRVTVCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLN 60 +LFDYVI GGGSAGATLAARLSEDPR++VCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLN Sbjct: 1 MLFDYVIVGGGSAGATLAARLSEDPRISVCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLN 60 Query: 61 NWALQTTPQAGLNGRRGYQPRGRALGGSSAINAMLYIRGQRQDYDGWAQAGCPGWDWESV 120 NWALQTTPQ GLNGRRGYQPRGRALGGSSAINAMLY+RGQRQDYDGWAQAGCPGWDWESV Sbjct: 61 NWALQTTPQPGLNGRRGYQPRGRALGGSSAINAMLYVRGQRQDYDGWAQAGCPGWDWESV 120 Query: 121 LPYFKRSENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEAAQARGYPLCQDFNTGDNE 180 LPYFKR+ENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEA QARG+ LCQDFNTGDNE Sbjct: 121 LPYFKRAENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEAGQARGHRLCQDFNTGDNE 180 Query: 181 GVGLYQVTQFHTSDHRGERCSAAAAYLHPVMGQRPNLTVLRQVRAQRVLFEGKRAVGVAY 240 GVGLYQVTQFHT HRGERCSAAAAYLHPVMGQRPNL+VLRQVRAQR+LFEGKRA+GVAY Sbjct: 181 GVGLYQVTQFHTPAHRGERCSAAAAYLHPVMGQRPNLSVLRQVRAQRLLFEGKRAIGVAY 240 Query: 241 RQQQQEAQVRATREVIIAAGAFGSPQLLQLSGVGRPEDILPHGIALHHALPGVGQNLQDH 300 RQQQ +AQVRA REVIIAAGAFGSPQLLQLSGVGR +DILPHGIALHH LPGVGQNLQDH Sbjct: 241 RQQQGDAQVRAAREVIIAAGAFGSPQLLQLSGVGRSDDILPHGIALHHPLPGVGQNLQDH 300 Query: 301 LDFTQGWTTRDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPELERP 360 LDFTQGWTTRDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPEL+RP Sbjct: 301 LDFTQGWTTRDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPELDRP 360 Query: 361 DIQLHFCIAIVDDHARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLIDAGYLSDP 420 DIQLHFCIAIVDDHARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLID GYLSDP Sbjct: 361 DIQLHFCIAIVDDHARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLIDPGYLSDP 420 Query: 421 RDLATMIAGARIARQIVMTEPLRRYCRRELFGGREGMDDAAWEAMIRQRADTIYHPVGTC 480 RDLATMI GAR+ARQIVMTEPLR YCRRELFGGR+ MDDA WE+MIR RADTIYHP GTC Sbjct: 421 RDLATMIDGARMARQIVMTEPLRHYCRRELFGGRDDMDDAQWESMIRHRADTIYHPAGTC 480 Query: 481 RMGEDQMAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADMIRASQNHA 540 RMGED MAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADMIRAS A Sbjct: 481 RMGEDAMAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADMIRASHEQA 540 Query: 541 EAA 543 AA Sbjct: 541 RAA 543 >gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 539 Score = 335 bits (858), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 210/541 (38%), Positives = 286/541 (52%), Gaps = 22/541 (4%) Query: 3 FDYVIAGGGSAGATLAARLSEDPRVTVCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLNNW 62 +DY+I G G+AG +A RLS V L+EAG + D + + P + + +W Sbjct: 7 YDYIIIGAGTAGCVMANRLSRKTGKKVLLIEAGAKDDYIWIHIPVGYLYCINN--PRTDW 64 Query: 63 ALQTTPQAGLNGRRGYQPRGRALGGSSAINAMLYIRGQRQDYDGWAQ-AGCPGWDWESVL 121 +T AGLNGR PRG+ LGG S+IN M+Y+RGQ +DYD WA G W W++VL Sbjct: 65 MFRTEADAGLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWADVTGDDSWRWQNVL 124 Query: 122 PYFKRSENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEAAQARGYPLCQDFNTGDNEG 181 P FK+SE+ GA +HGA G +V +Q I AF +AA G P +DFN GDNEG Sbjct: 125 PLFKKSEDYHLGAGQFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCGDNEG 184 Query: 182 VGLYQVTQFHTSDHRGERCSAAAAYLHPVMGQRPNLTVLRQVRAQRVLFE----GKRAVG 237 G + V Q RG R +A+ A+L P M + NLT++ R+ E G G Sbjct: 185 CGYFDVNQ-----KRGVRWNASKAFLRPAM-KDGNLTIMTGSHVSRLRMEQGEQGPVCTG 238 Query: 238 VAYRQQQQEAQVRATREVIIAAGAFGSPQLLQLSGVGRPEDILPHGIALHHALPGVGQNL 297 V + A +E I+ AGA GSP +LQ+SG+ P + H I + HALPGVG+NL Sbjct: 239 VEF-TGGGSAWFAEAKETILCAGAIGSPHILQMSGIADPALLQQHQIPVVHALPGVGENL 297 Query: 298 QDHLDFTQGWTT---RDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTR 354 QDHL + + + +VG +++ Q L + G ++ ++ AF K+ Sbjct: 298 QDHLQMRMVFKVNGAKTLNAMASTLVGKMQIGLQYLFTQ---SGPMSMAPSQLGAFAKSD 354 Query: 355 PELERPDIQLHFCIAIVDDHARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLIDA 414 + ++Q H ++ LHA F+ +C LRP SRG V L S D P I Sbjct: 355 AQQASANLQYHVQPLSLEKFGDPLHAFPAFTASVCNLRPTSRGHVRLASGDHALAPRITT 414 Query: 415 GYLSDPRDLATMIAGARIARQIVMTEPLRRYCRRELFGGREGMDDAAWEAMIRQRADTIY 474 YLS DL R+ R+I + L RY E G + + TI+ Sbjct: 415 NYLSTEEDLKVAANALRLTRRIAASPALSRYRPEEYKPGAHYETEEDLYRAAGEVGTTIF 474 Query: 475 HPVGTCRMG--EDQMAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADM 532 HPVGTCRMG +D MAVVDAQ RV G+ GL V DASVMP++ SGNTN+PT+MIAEK A Sbjct: 475 HPVGTCRMGRSDDAMAVVDAQGRVRGVGGLSVADASVMPSITSGNTNSPTVMIAEKVASS 534 Query: 533 I 533 + Sbjct: 535 L 535 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,808,047 Number of extensions: 81216 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 2 Length of query: 543 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 453 Effective length of database: 1,193,720 Effective search space: 540755160 Effective search space used: 540755160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)