BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0041.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 674 0.0 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 147 1e-36 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 129 3e-31 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 78 1e-15 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 674 bits (1740), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/387 (90%), Positives = 363/387 (93%), Gaps = 1/387 (0%) Query: 1 MNPSVPAGSAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDHLLVAH 60 MNPSVPAG APIPVSLLTGFLGSGKTTLLNHLITQPEMK+TLVIINEFGEIGLDHLLVAH Sbjct: 1 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAH 60 Query: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILHT 120 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGG+RKF+RVVIETTGLASP PILHT Sbjct: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 Query: 121 LMTDAFISERYRLDGVIVTVDAVNGMSTLDHHPEAIQQAAVADRLLLTKTDLVDADTLSA 180 LMTDAFI+ERYRLDGVIVTVDAVNGMSTLDHH EA+QQAAVADRLLLTKTDLVDA TL+A Sbjct: 121 LMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA 180 Query: 181 LQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEK-EAHAH 239 LQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAE+ Sbjct: 181 LQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQARHGHG 240 Query: 240 HAHHHHGHGHEHGHHDHHGHHHDDHAHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV 299 H HHHGH H HGH H HH DH HDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV Sbjct: 241 HHGHHHGHDHHHGHDHGHDHHGHDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV 300 Query: 300 GFKGPNILRIKGILNLKGEDKPTVIHGVQHIFHPTATLPEWPSEDRRSRIVFITRDVERA 359 GFKGPNILRIKGILNLKGE PTVIHGVQHIFHPTATLP+WPSEDRRSRIVFITRDVERA Sbjct: 301 GFKGPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVERA 360 Query: 360 TIERSFDAFMQAQAIEREAREQRQSPS 386 TIERSFDAFMQAQAIEREARE++ +PS Sbjct: 361 TIERSFDAFMQAQAIEREAREKKAAPS 387 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 147 bits (371), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 92/251 (36%), Positives = 138/251 (54%), Gaps = 36/251 (14%) Query: 7 AGSAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDHLLVAHS----- 61 A +PV++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV Sbjct: 8 AADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLA 67 Query: 62 -QEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILHT 120 EE +VEMS+GC+CCT+R DL ++ + +R+FD++VIE+TG++ P P+ T Sbjct: 68 RTEEKLVEMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAET 121 Query: 121 L-MTD---AFISERYRLDGVIVTVDAVNGMS------TLDHHPEA-------------IQ 157 TD A +S+ RLD ++ VDA N + +LD ++ I+ Sbjct: 122 FTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIE 181 Query: 158 QAAVADRLLLTKTDLVDADTLSALQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGE 217 Q D ++L K DLV AD L L A L LNP AR ++ + G++A ++LD GLF E Sbjct: 182 QIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFD-FE 240 Query: 218 KMPDVARWLNE 228 + WL E Sbjct: 241 QASQAPSWLQE 251 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 88/253 (34%), Positives = 137/253 (54%), Gaps = 39/253 (15%) Query: 1 MNPSVP-----AGSAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDH 55 MN P A + IPV++L+GFLGSGKTTLLNH++ VI+N+ E+ +D Sbjct: 1 MNEPAPEVLLNAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDG 60 Query: 56 LLVAHSQE-----ETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTG 110 V + E + +VEMS+GC+CCT+R DL + + + A G +FD ++IE+TG Sbjct: 61 DDVRRNTELYRGNDELVEMSNGCICCTLRADLLEQVSAL----ARSG--RFDYLLIESTG 114 Query: 111 LASPTPILHT---LMTDAF-ISERYRLDGVIVTVDA---------------VNGMSTLDH 151 ++ P P+ T L +D F +SE RLD ++ V+ +G ST Sbjct: 115 ISEPIPVAETFAFLDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPL 174 Query: 152 HPEAIQQAAVADRLLLTKTDLVDADTLSALQARLRTLNPGARQLQPQVGQIAAAELLDAG 211 I+Q A+ +L+++ DL+DA ++L+A L+ LNP AR L G++ ++LD Sbjct: 175 SDLLIEQVEYANVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTR 234 Query: 212 LFKPGEKMPDVAR 224 LF +P +AR Sbjct: 235 LF----DLPSLAR 243 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 63/210 (30%), Positives = 102/210 (48%), Gaps = 17/210 (8%) Query: 1 MNPSVPAGSAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLD-HLLVA 59 + P++P PIPV+++TGFLG+GKTTLL L+ + + + ++INEFGE+ +D LL A Sbjct: 10 LAPALP----PIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRA 65 Query: 60 HSQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILH 119 + V++ GD + + A Q D V+IET+GLA P+ ++ Sbjct: 66 EGDAQGQVQIQDFAHGLIAYGDDAHFVPAMLALAARRAQ--IDHVLIETSGLALPSAVME 123 Query: 120 TLMTDAFISERYRLDGVIVTVDAVNGMSTLDHHPEAI---------QQAAVADRLLLTKT 170 L A ++ + LD + VD + +A+ QQ AD ++L K Sbjct: 124 VLQ-GAEMARHFVLDATLAVVDTPLLLEDAFASADAVGAATAQLFRQQLECADVVVLNKI 182 Query: 171 DLVDADTLSALQARLRTLNPGARQLQPQVG 200 D ++ L + +R L P R L+ G Sbjct: 183 DGMEEAPLLQAEQAVRALAPNVRFLELAYG 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,148,545 Number of extensions: 45735 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 4 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)