BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0028.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 599 e-172 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 295 4e-81 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 282 3e-77 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 83 3e-17 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 72 5e-14 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 599 bits (1544), Expect = e-172, Method: Compositional matrix adjust. Identities = 307/329 (93%), Positives = 315/329 (95%), Gaps = 1/329 (0%) Query: 1 MTQTAAAQATHTTLDSLPPPSGTPP-RLVARIRDDAEAIVAARKQAEDFSREAALRDRER 59 MTQTAAA A+ TTLDS PPP + P RLVARIRDDAEAI AARK AEDFSREAALRDRER Sbjct: 1 MTQTAAAHASLTTLDSFPPPPSSTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRER 60 Query: 60 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE 119 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE Sbjct: 61 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE 120 Query: 120 LIRVNGSESQKRFFFDRVLQGDRFGNALAELGTKTSHDRKTRLTRNEHGPGYRINGRKFY 179 LIRVNGSESQKRFFF+RVLQGDRFGNALAELGTKTSHDRKTRL+RN HGPGYRI+GRKFY Sbjct: 121 LIRVNGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFY 180 Query: 180 ATGALYADWVPTSVIDEEGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVQVP 239 ATGALYADWVPTSVID++GVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENV VP Sbjct: 181 ATGALYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVP 240 Query: 240 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLQFVRTRSRPWVDSGVERAG 299 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLL FVRTRSR WVDSGVERA Sbjct: 241 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAV 300 Query: 300 DDPLTIREVGDLTIQLHAAEALVRRAGRK 328 DDPLTIREVGDLTIQLHAAEAL+RRAGRK Sbjct: 301 DDPLTIREVGDLTIQLHAAEALLRRAGRK 329 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 295 bits (755), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 150/293 (51%), Positives = 198/293 (67%), Gaps = 2/293 (0%) Query: 26 RLVARIRDDAEAIVAARKQAEDFSREAALRDRERKLPWDELERYVRSGLWGITVPKEYGG 85 R RI ++ A+ A + A +F+ AA RDRER LP++++ER+ +SGLW ITVP+ +GG Sbjct: 15 RPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWAITVPRSHGG 74 Query: 86 AGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGSESQKRFFFDRVLQGDRFGN 145 G S+ T+AEVI ISAAD SIGQIPQNHF + LI S+ Q+R FF VL G R GN Sbjct: 75 IGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQRLFFAEVLDGARLGN 134 Query: 146 ALAELGTKTSHDRKTR--LTRNEHGPGYRINGRKFYATGALYADWVPTSVIDEEGVQQLA 203 A E K D +TR L R Y +NG +FY+TGALYA W+P+ ID++G + Sbjct: 135 AGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDDDGQPLVV 194 Query: 204 IVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVQVPADAVIGFATAFSRPTQIGPMAQIQ 263 R APG+ +IDDWS FGQRTT SG+ VF+NV+VPA V+ A +P IGP++Q+ Sbjct: 195 YTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQPNNIGPVSQLI 254 Query: 264 HAAIDLGIARAAYADLLQFVRTRSRPWVDSGVERAGDDPLTIREVGDLTIQLH 316 AAID GIA AA D + FV TRSRP+VDSG+ERA DDP +R++G+L+++LH Sbjct: 255 QAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLH 307 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 282 bits (721), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 158/330 (47%), Positives = 208/330 (63%), Gaps = 8/330 (2%) Query: 7 AQATHTTLDSLPPPSGTP----PRLVARIRDDAEAIVAARKQAEDFSREAALRDRERKLP 62 A + H+T + P G P P V IRDDAEAI A A ++ A+ RD+ R+ P Sbjct: 6 APSLHSTSSAAAPVPGLPRASQPAHV--IRDDAEAIAVAHALAPRLAQGASERDQLRRWP 63 Query: 63 WDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIR 122 +E+E Y +SGLW I VP+ +GGA VSY TLAEVIA ISAAD S+GQI QNH V +R Sbjct: 64 VEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVR 123 Query: 123 VNGSESQKRFFFDRVLQGDRFGNALAELGTKTSHDRKTRLTRNEHGPGYRINGRKFYATG 182 Q+R FF L+G R+GNA +E G+K + D +TR + G + G+KFY+TG Sbjct: 124 TVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFV--DAGDHVIVTGKKFYSTG 181 Query: 183 ALYADWVPTSVIDEEGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVQVPADA 242 AL A +VP DEE + R+APGLT+IDDWS FGQRTT SG+ + + V+VP Sbjct: 182 ALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTH 241 Query: 243 VIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLQFVRTRSRPWVDSGVERAGDDP 302 +I + P+ G + QI AA+D+GIARAA AD FVRT+SRPW+DS + A +DP Sbjct: 242 LIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDP 301 Query: 303 LTIREVGDLTIQLHAAEALVRRAGRKXSVA 332 +I+ +GDL I+LHA+EAL+ RAGR A Sbjct: 302 YSIQAIGDLQIRLHASEALLERAGRAIDAA 331 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 70/227 (30%), Positives = 105/227 (46%), Gaps = 18/227 (7%) Query: 33 DDAEAIVAARKQAEDFSRE-----AALRDRERKLPWDELERYVRSGLWGITVPKEYGGAG 87 D E I A R+ F+ AA DR + P D ++ G+ GITV +EYGGAG Sbjct: 10 DHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAG 69 Query: 88 VSYGTLAEVIAT--ISAADGSIGQIPQNHF-YAVELIRVNGSESQKRFFFDRVLQGDRFG 144 + Y LA +IA IS A S+G H V I+ NG+E QKR + R++ GD G Sbjct: 70 LGY--LAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIG 127 Query: 145 NALAELGTKTSHDRKTRLTRNEHGPGYRINGRKFYATGALYAD----WVPTSVIDEEGVQ 200 S +L ++ G Y +NG K + T AD + T + E G + Sbjct: 128 ALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL--EAGAR 185 Query: 201 QLA--IVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVQVPADAVIG 245 + +V + G ++ G R + +G VF++ +VP + V+G Sbjct: 186 GMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLG 232 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 78/288 (27%), Positives = 125/288 (43%), Gaps = 31/288 (10%) Query: 56 DRERKLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQI--PQN 113 DR P + L+ G G+ VP+++GGAG+ Y +L + I+A DG+ I QN Sbjct: 30 DRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQN 89 Query: 114 HFYAVELIRVNGSESQKRFFFDRVLQGDRFGN-ALAELGTKTSHDRKTRLTRNEH-GPGY 171 A + GS +QK + + +G+ G L E T D TR E G + Sbjct: 90 SL-ACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTE--PHTGSDAAAIRTRAERDGDDF 146 Query: 172 RINGRKFYATGALYADW-VPTSVID----EEGVQQLAIVPRHAPGLTIIDDWSGFGQRTT 226 +NG K + T +A + +V D ++G+ +VP PG + GQ + Sbjct: 147 ILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF-LVPCDTPGFVVGRSEEKMGQHAS 205 Query: 227 GSGSAVFENVQVPADAVIG-------FATAFSRPTQIGPMAQIQHAAIDLGIARAAYADL 279 + + +N +VPA A++G A + +IG AQ +G+ARAA+ Sbjct: 206 DTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQ------SVGMARAAFEAA 259 Query: 280 LQFVRTRSRPWVDSGVERAGDDPLTIREVGDLTIQLHAAEALVRRAGR 327 + + R R GV + R + D+ L AA +V RA + Sbjct: 260 VSYARQRE----SFGVPIIEHQAVNFR-LADMNTLLDAARLMVWRAAQ 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,095,245 Number of extensions: 48516 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 6 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)