BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0025.p (273 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 513 e-147 gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 278 3e-76 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 259 2e-70 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 203 1e-53 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 158 6e-40 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 513 bits (1322), Expect = e-147, Method: Compositional matrix adjust. Identities = 249/276 (90%), Positives = 258/276 (93%), Gaps = 3/276 (1%) Query: 1 MKSIVTNWL---SHLAIGVLASASITAQAATDQVIRVGVTSGPHAQIFEQARRVFERDNP 57 MKSI WL S L + +LAS + AQAA DQVIRVGVTSGPHAQIFEQARRVFERDNP Sbjct: 1 MKSIAATWLRRLSTLMVSMLASLVLNAQAAADQVIRVGVTSGPHAQIFEQARRVFERDNP 60 Query: 58 GYKVKIIEFNDYIQPNAALDAGELDANSYQHRPFLNAQIKARGYKLYAEGKTMIGPMAIY 117 GYKVKIIEFNDYIQPNAALDAGELDANSYQHRPFLNAQIK RGYKLYAEGKTMIGPMAIY Sbjct: 61 GYKVKIIEFNDYIQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIY 120 Query: 118 SRKYRKLEDLPVGAKIGIPNDPANESRVLLLLQKQGLIKLREGIDPLTGTNATPIDITEN 177 SRKYRKLED+PVGA+IGIPNDPANESRVLLLLQK G IKLR GIDPLTGTNATPIDITEN Sbjct: 121 SRKYRKLEDVPVGARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITEN 180 Query: 178 PKKWKFVEIDAAQLPRTLDDLDASAINADYASKAGFNPARDSLLVESGDSPYACLIAVRD 237 PKKWKFVEIDAAQLPRTLDDLDASA+NADYA+KAG NPARDSL+VESGDSPYACLIAVR+ Sbjct: 181 PKKWKFVEIDAAQLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVRE 240 Query: 238 KDRAQPWLGKLVQAYQSPEVKRYIETEFKGGILAAW 273 KDRAQPWLGKLVQAYQSPEVKRYIETEFKGGILAAW Sbjct: 241 KDRAQPWLGKLVQAYQSPEVKRYIETEFKGGILAAW 276 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 278 bits (711), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 137/257 (53%), Positives = 190/257 (73%), Gaps = 6/257 (2%) Query: 17 LASASITAQAATDQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIEFNDYIQPNAAL 76 LA+ ++A A I++GVT+GPHA+I EQ +++ E+D G ++K+IEF DYIQPNAAL Sbjct: 15 LAAGLVSAPAFAQDQIKMGVTAGPHAEIMEQVKKLLEKD--GVQMKVIEFTDYIQPNAAL 72 Query: 77 DAGELDANSYQHRPFLNAQIKARGYKLYAEGKTMIGPMAIYSRKYRKLEDLPVGAKIGIP 136 AG+LDANSYQH+P+L+AQIK RGYK + G T+ PM +YS+K + L DL GA++G+P Sbjct: 73 AAGDLDANSYQHQPYLDAQIKDRGYKFVSVGSTITFPMGVYSKKIKSLNDLKQGARVGVP 132 Query: 137 NDPANESRVLLLLQKQGLIKLREGIDPLTGTNATPIDITENPKKWKFVEIDAAQLPRTLD 196 NDP N R LL+LQ +G+IKL+ G ATP+DI ENPKK K VE+DAAQLPR+LD Sbjct: 133 NDPTNGGRALLVLQAKGVIKLKAD----AGLKATPLDIVENPKKIKIVELDAAQLPRSLD 188 Query: 197 DLDASAINADYASKAGFNPARDSLLVESGDSPYACLIAVRDKDRAQPWLGKLVQAYQSPE 256 D DA+ IN +YA AG +P +D++ VE+ PYA +IAVR D+ +PW+ KLV+AY SPE Sbjct: 189 DFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAKLVKAYHSPE 248 Query: 257 VKRYIETEFKGGILAAW 273 VK+++ ++KG ++ +W Sbjct: 249 VKKFVLEKYKGSVITSW 265 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 259 bits (661), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 140/273 (51%), Positives = 192/273 (70%), Gaps = 5/273 (1%) Query: 3 SIVTNWLSHLAIGVLASASI--TAQAATDQVIRVGVTSGPHAQIFEQARRVFERDNPGYK 60 S N AIG L+ A + QA Q I+VG++ G IF+ ++V RD G + Sbjct: 5 SCFKNIKRLAAIGALSLALVPQLGQAQDKQKIKVGISVGSAEAIFDVVKKVAARD--GLE 62 Query: 61 VKIIEFNDYIQPNAALDAGELDANSYQHRPFLNAQIKARGYKLYAEGKTMIGPMAIYSRK 120 ++ + F+DY+QPNAAL AG+LDAN++QHRP+L +QI ARGY++ G T+ P+ IYSRK Sbjct: 63 IQTVVFSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRK 122 Query: 121 YRKLEDLPVGAKIGIPNDPANESRVLLLLQKQGLIKLREGIDPLTGTNATPIDITENPKK 180 +R +E LP GA IGI NDP+N +R LLLLQK GLI+L+ G+ G NATP+D+ ENP+K Sbjct: 123 FRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGE-NGVNATPLDVIENPRK 181 Query: 181 WKFVEIDAAQLPRTLDDLDASAINADYASKAGFNPARDSLLVESGDSPYACLIAVRDKDR 240 K VE+DAAQLPR+LDDL A++IN DYA KAG + +D++ VE YA +IA R +D+ Sbjct: 182 LKLVELDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDK 241 Query: 241 AQPWLGKLVQAYQSPEVKRYIETEFKGGILAAW 273 +PW+ KLVQAY S EV+++IETEFKG ++ A+ Sbjct: 242 DRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF 274 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 203 bits (516), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 107/271 (39%), Positives = 161/271 (59%), Gaps = 9/271 (3%) Query: 4 IVTNWLSHLAIGVLASASITAQAATDQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKI 63 ++ + HL G + S+ A AA VI T PHA+I E + ++ +VK+ Sbjct: 1 MIRKLIPHLFAGAALAFSVGAHAADKLVI--AATPVPHAEILEHIKPALAKEGIDLQVKV 58 Query: 64 IEFNDYIQPNAALDAGELDANSYQHRPFLNAQIKARGYKLYAE-GKTMIGPMAIYSRKYR 122 F DY+QP A + ++D N + H+P+L+ K+ + K + P A YS+KY+ Sbjct: 59 --FTDYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYK 116 Query: 123 KLEDLPVGAKIGIPNDPANESRVLLLLQKQGLIKLREGIDPLTGTNATPIDITENPKKWK 182 K+ D+P GA I IPNDP+N R LLLL + GLIKL++ T +AT DI ENPKK K Sbjct: 117 KIADIPNGATIAIPNDPSNSGRALLLLARNGLIKLKDN----TNISATQKDIVENPKKLK 172 Query: 183 FVEIDAAQLPRTLDDLDASAINADYASKAGFNPARDSLLVESGDSPYACLIAVRDKDRAQ 242 F ++AA LPR L+ +D + IN +YA +A NP +DSL +E +SPYA L+ R+ ++ Sbjct: 173 FRALEAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDS 232 Query: 243 PWLGKLVQAYQSPEVKRYIETEFKGGILAAW 273 P + KL A SP+VK++IE +++G I+ A+ Sbjct: 233 PAIKKLAAALNSPDVKKFIEEKYQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 158 bits (399), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 100/255 (39%), Positives = 140/255 (54%), Gaps = 14/255 (5%) Query: 9 LSHLAIGVLASASITAQAATDQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIEFND 68 SHL L S+ A A D+ +R+GV G + A + E G V+++EF D Sbjct: 3 FSHLVFLSLTVLSLQAAQAADK-LRIGVVPGAYGDSVAVAAK--EAKRQGIDVQVVEFTD 59 Query: 69 YIQPNAALDAGELDANSYQHRPFLNAQIKARGYKLYAEGKTMIGPMAIYSRKYRKLEDLP 128 + PN ALDAG+LD N +QH+PFL+ I+ GYKL + G ++ + +YS KY+ L LP Sbjct: 60 WTTPNVALDAGDLDLNYFQHQPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALP 119 Query: 129 VGAKIGIPNDPANESRVLLLLQKQGLIKLREGIDPLTGTNATPIDITENPKKWKFVEIDA 188 GAK+GI NDP N+ R LLLLQ GLIKL+ P G + DI +NP+K FVE++ Sbjct: 120 QGAKVGIANDPVNQGRALLLLQNVGLIKLK----PKVGYLGSIDDIIDNPRKLSFVEVEG 175 Query: 189 AQLPRTLDDLDASAINADY--ASKAGFNPARDSLLVESG--DSPYACLIAVRDKDRAQPW 244 QL R D+D + + A+KA F+P+ S L SG D+ +A R P Sbjct: 176 PQLVRITPDVDLAQGYPHFIVAAKA-FDPS--SGLAYSGIEDARFAIQFVTRANRVNDPV 232 Query: 245 LGKLVQAYQSPEVKR 259 + K +Q YQ+ R Sbjct: 233 VQKFIQIYQNSAAVR 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 839,109 Number of extensions: 32760 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 5 Length of query: 273 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 189 Effective length of database: 1,222,130 Effective search space: 230982570 Effective search space used: 230982570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)