BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0015.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311223|ref|YP_003775315.1| sigma-54 dependent transcriptio... 88 7e-19 >gi|300311223|ref|YP_003775315.1| sigma-54 dependent transcription activator protein [Herbaspirillum seropedicae SmR1] Length = 675 Score = 88.2 bits (217), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 83/289 (28%), Positives = 119/289 (41%), Gaps = 27/289 (9%) Query: 18 DGQPDPAASPYLQRLSSSYLRSLDQHHLDPADMRGPR----VLSSGELRQVQQGEEDFLR 73 DG PD A + SSSY MRG R LS +L Q + + Sbjct: 25 DGVPDLQAIERSHQRSSSY-----------GIMRGQRPDLDSLSRADLAQTLEANQTLSG 73 Query: 74 ASGQCLSVLHQTVREADYCVMLTNAGGTTIDYRVDRERRHDFKRAGLYLGSCWSESEEGT 133 + + L+ +R V+L++A GT + D + R L G WSE GT Sbjct: 74 HARPVMETLYDQIRNTHSMVILSDAEGTILHTLGDSDFLAKADRVALGPGGRWSEQLRGT 133 Query: 134 CGVAAVLQDALPITVHKSDHFRAAFTTLTCSAAPIFSPSGELIGVLDASAIRSPDARDSQ 193 + L + P TVH H+ A LTCSAAPIF G+++GVLD S + Sbjct: 134 NAIGTALFEQKPTTVHAQQHYLDANRFLTCSAAPIFDHRGQVLGVLDVSG----ECGSFH 189 Query: 194 NLVNRIVQQSATLIEDGYFLNAMACAWVLLVHRSRHYVEAQPEILIAFDALGNIIAANRR 253 +V+ SA +IE+ + L H +V E +++F G +AANR Sbjct: 190 KHTMALVRMSAQMIENHLLAGSFPDCITLHFHSRAEFVGTLVEGIVSFTPGGRFLAANRS 249 Query: 254 ARE----CIAGLQQLPRPMGEIFDLQFERLLDS--RALQGLHLLRLHGG 296 A+ +A LQ +F + L + +A GL L LH G Sbjct: 250 AQFQLGLTLAALQS--HTFASLFGVPLSLLFEHHRKAAPGLLELCLHNG 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,116 Number of extensions: 34148 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 1 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)