BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0011.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 116 2e-27 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 114 8e-27 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 112 3e-26 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 108 3e-25 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 106 2e-24 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 70/239 (29%), Positives = 116/239 (48%), Gaps = 5/239 (2%) Query: 16 LSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALG 75 +S R+ +TG GIG A+ T + +V S R QG + G Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 76 DLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLI 135 ++++ + + G +D LV NAG + ++ DW +D L F + Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 136 TACREPLQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVT 195 E + G GR++ +SS + G++ + + AKAGI +LA +VA GVT Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNY---STAKAGIHGFTMALAQEVATKGVT 177 Query: 196 VNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVTG 254 VN V+PGY+ +D + AI P + ++ IP++RLG+P+EIA+++A++ S Y TG Sbjct: 178 VNTVSPGYI-GTDMVR-AIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATG 234 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 114 bits (285), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 123/241 (51%), Gaps = 9/241 (3%) Query: 21 ILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALGDLADA 80 I +TG++RGIG A+A +A +VLH + VA + A G Q + D+A+ Sbjct: 9 IFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANR 68 Query: 81 HTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLITACRE 140 AAQ+ A + +G +V NAG + DW L L GF+ ++ Sbjct: 69 EQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVM 128 Query: 141 PL-QASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVTVNAV 199 P+ + GR+V ++S + G+ + +AAKAG+ K+LA+++A +TVN V Sbjct: 129 PMVRRRAAGRIVTLASVSGLIGNRGQVNY---SAAKAGVIGATKALALELAKRDITVNCV 185 Query: 200 APGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVTGQVWHV 259 APG + + ID L IP +R G+P+E+A +AFL+SP A+Y+T QV V Sbjct: 186 APGLIDTDMTEDAPIDE-----ILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV 240 Query: 260 D 260 + Sbjct: 241 N 241 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 112 bits (280), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 84/249 (33%), Positives = 132/249 (53%), Gaps = 13/249 (5%) Query: 16 LSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTL-AL 74 L +V L+TGA+RGIG A+AT +A ++ A SES A+ + A G + A+ Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGA-AAITEYLAALGPEAGRGAV 64 Query: 75 GDLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRL 134 ++ DA + L + +G L LV NAG + + D +W + L RL Sbjct: 65 LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 135 ITACREPLQASGQGRVVAVSSFVAHVFRLGESGFPAS---AAAKAGIEALVKSLAVQVAA 191 A + + GR++ ++S V GE+G P AAAKAG+ + ++LA ++ + Sbjct: 125 SRAVLRGMMKARTGRIINITSVV------GEAGNPGQMNYAAAKAGVAGMSRALAREIGS 178 Query: 192 AGVTVNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASY 251 +TVN VAPG++ +D + A++ A L +IPL RLG +EIA +AFL SP A+Y Sbjct: 179 RNITVNCVAPGFI-DTDMTR-ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAY 236 Query: 252 VTGQVWHVD 260 +TG +V+ Sbjct: 237 ITGTTLNVN 245 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 108 bits (271), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 93/249 (37%), Positives = 122/249 (48%), Gaps = 10/249 (4%) Query: 14 SPLSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLA 73 + L +V LITGA+ GIG A A A AL+L AR + Q A C AQG + ++ Sbjct: 2 NTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALIC-AQGGRASVV 60 Query: 74 LGDLADAHTAAQLRDAALTHYGRLDALVANAG-FADRKRIGELDDADWSRSLDALLGGFF 132 GD+ +A T + AL +G LD + NAG K + E+ W +L A L F Sbjct: 61 AGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAF 120 Query: 133 RLITACREP-LQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAA 191 L C+ P + A G G +V SSFV G G A AAKAG+ LVK +A A Sbjct: 121 -LGARCQIPAMLARGAGAIVFTSSFVGS--SAGLPGMAAYGAAKAGLMGLVKGIAADYAV 177 Query: 192 AGVTVNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASY 251 G+ NA+ PG V DP Q A ++R+ QP+EIA FL SP AS+ Sbjct: 178 QGIRANALLPGGVDTDMGG----DPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASF 233 Query: 252 VTGQVWHVD 260 VTG + D Sbjct: 234 VTGTALYAD 242 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 91/247 (36%), Positives = 131/247 (53%), Gaps = 21/247 (8%) Query: 16 LSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALG 75 L + L+TG +GIGAAVA + A L AR + L A + A G++ TL Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVA------LGARVIAAGLPPFAHEEFA-GSEVTLVEM 60 Query: 76 DLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLI 135 ++A + AAL +LD +V AG KR EL+ + + + L G R+ Sbjct: 61 NVASEESV----RAALAGLTQLDIVVNCAGII--KRGAELETEVFEQVIAVNLTGTMRVC 114 Query: 136 TACREPLQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVT 195 A RE L+ S +G +V +S ++ G P +A+K G+ L KSLA+ AA G+ Sbjct: 115 AAARELLRQS-RGCIVNTASMLSF---FGGGLVPGYSASKGGVAQLTKSLAIAYAADGIR 170 Query: 196 VNAVAPGYVQKSDPAKSAI--DPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVT 253 VNAVAPG++ + P A+ DP LAR PL R GQP+++ V+AFL SPAAS++T Sbjct: 171 VNAVAPGWI--ATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMT 228 Query: 254 GQVWHVD 260 G + VD Sbjct: 229 GAIVPVD 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 615,607 Number of extensions: 22324 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 159 Number of HSP's successfully gapped: 39 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)