BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0003.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 832 0.0 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 294 1e-80 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 118 1e-27 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 107 1e-24 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 106 4e-24 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 832 bits (2149), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/475 (93%), Positives = 463/475 (97%) Query: 1 MSSQPSTSGTSDPAHHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 MSSQP+ GT+ P+ HIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD Sbjct: 1 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 Query: 61 LNTTPAASVWIVTAYQLAMMVALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLP 120 L TTPAASVWIVTAYQLAMMVALLPLAALGEI+GYRRIYIWGLVLFTAASLLCAVAWSLP Sbjct: 61 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP 120 Query: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA Sbjct: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 Query: 181 IAPWPWLFAVNVPLGVAAVILALPSLPHTALSTHAFDLRSAVLNAAAFGLLILAIGEGAH 240 IAPWPWLFAVNVPLGV AV+LA+PSLPHT+L+ HAFDLRSA+LNAAAFGLLILAIGEGAH Sbjct: 181 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH 240 Query: 241 QAEASVVGLELGAAIVCGLFLLKRELSHPAPMLPVDLFRHPMFTLSAITAVCSFAAQGLA 300 QA+ASVVGLEL AAI+CGLFLLKRELSHPAPMLPVDLFRHPMF+LSA+TAVCSFAAQGLA Sbjct: 241 QAQASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA 300 Query: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRLSDRYPVGLLGGVGLALLCAG 360 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGR+SDRYPVG+LGGVGLALLCAG Sbjct: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAG 360 Query: 361 LLSMTFMPAAPTVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 LLSMTFMPA+P+VWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL Sbjct: 361 LLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 Query: 421 GQSVGAALVALCFNLSETQGSRFALGFGAAFAGVACIASFLRMLTSNPYAPPLKK 475 GQSVGAALVALCFNLS+TQGSR ALGFGAAFAG ACIASFLR+LT NPYAPP KK Sbjct: 421 GQSVGAALVALCFNLSQTQGSRLALGFGAAFAGAACIASFLRLLTRNPYAPPPKK 475 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 294 bits (752), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 181/416 (43%), Positives = 257/416 (61%), Gaps = 8/416 (1%) Query: 22 EGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLNTTPAASVWIVTAYQLAMMV 81 EGLP +QR RA+LA+ A +A D I N ALP ++ L T+ A S+W+ AY LA + Sbjct: 20 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 79 Query: 82 ALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNT 141 L AAL +G+R + GL +FT ASL CA++ S+ L++ R LQGLGGA ++S+ Sbjct: 80 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 139 Query: 142 ALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVAAVIL 201 A+ R I+P R LGR +GLN ++VA + VGP + +LA+ W W+FA+NVPLGV AV L Sbjct: 140 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWL 199 Query: 202 ALPSLPHTALSTHA-FDLRSAVLNAAAFGLLILAIGEGAHQAE-------ASVVGLELGA 253 A ++P T A FDL A+ +A A G LI+A A AE A Sbjct: 200 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVT 259 Query: 254 AIVCGLFLLKRELSHPAPMLPVDLFRHPMFTLSAITAVCSFAAQGLAFVSLPFFFHHDLG 313 A++ GL ++ + P+LP+D+F +P F+L+A+T++ SF QG+AFV+LPF F + G Sbjct: 260 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 319 Query: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRLSDRYPVGLLGGVGLALLCAGLLSMTFMPAAPTV 373 + E L TPWP+ + ++AP AGRL+DR+ LL +GLAL GL+ + +P Sbjct: 320 YTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQAQP 379 Query: 374 WEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALV 429 W+I WR L+CGMGFGFFQSPN + ++ + RSG ASG++A R GQ +GAAL+ Sbjct: 380 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALL 435 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 118 bits (295), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 117/374 (31%), Positives = 174/374 (46%), Gaps = 7/374 (1%) Query: 3 SQPSTSGTSDPAHHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLN 62 S PST + A H AG L P ++AL I +A LD + N L +A L+ Sbjct: 2 SCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLD 61 Query: 63 TTPAASVWIVTAYQLAMMVALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLPTL 122 + VWIV Y L LL AL + G RR+Y+ GL LF ASLLC A + P L Sbjct: 62 APLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFL 121 Query: 123 VMARFLQGLGGAGIMSVNTALIRFIYP-TRSLGRGVGLNTLIVAIAFTVGPSVASGILAI 181 ++AR LQGLG A M + +L+ +Y R R +G+ + V +A VGP V ++ Sbjct: 122 IVARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHW 181 Query: 182 APWPWLFAVNVPLGVAAVILALPSLPHTALSTHAFDLRSAVLNAAAF-GLLILAIGEGAH 240 W +F +NVP+G+ +++A +P A +L S + GL + I + Sbjct: 182 LGWRSVFLINVPVGLVGLVMARKLIPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPVY 241 Query: 241 QAEASVVGLELGAAIVCGLFLLKRELSHPAPMLPVDLFRHPMF-TLSAITAVCSFAAQGL 299 + + L A+ G L++ EL AP+LP LF P F + I + +F G Sbjct: 242 GWTSPRILAGLAVALSAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGN 301 Query: 300 AFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRLSDRY--PVGLLGGVGLALL 357 F L F G ++ GL + P + ++GR+S + V LL G+ + + Sbjct: 302 LF-YLALFLQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTV 360 Query: 358 CAGLLSMTFMPAAP 371 L+M F PA P Sbjct: 361 FTA-LAMGFSPATP 373 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 118/429 (27%), Positives = 191/429 (44%), Gaps = 20/429 (4%) Query: 11 SDPAHHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLNTTPAASVW 70 +D AH +G+ LP + A L + + L LD I TALP + +L + W Sbjct: 5 TDSAHS---SGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYA-W 60 Query: 71 IVTAYQLAMMVALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQG 130 + TAY L ++ + LG+ G + I +++F AS LC +A S+ LV+AR LQG Sbjct: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 Query: 131 LGGAGIMSVNTALIRFIYPT-RSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFA 189 +GG ++ A + ++P R R + ++ IA VGPS+ + W W+F Sbjct: 121 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 180 Query: 190 VNVPLGVAAVILALPSLPHTALSTHAFDLR----SAVLNAAAFGLLILAIGEGAHQAEAS 245 N+P+GV ++ +PH HA +R A+L + + G L L + + S Sbjct: 181 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 240 Query: 246 VVGLELGAAIVCGLFLLKRELSHPAPMLPVDLFRH----PMFTLSAITAVCSFAAQGLAF 301 + + L ++ L K E P+LP ++ R +F LS + F+ A Sbjct: 241 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYA- 299 Query: 302 VSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRLSDRYPV-GLLGGVGLALLCAG 360 P F G S E GLL+TP + + + + GR+ R P L+ +G AL+ A Sbjct: 300 ---PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAAS 356 Query: 361 LLSMTFMPAAPTVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRS-GGASGIVATARL 419 L + + ML G G G PNL A G A+ ++ + R+ Sbjct: 357 CLGIVLCDRVTGKGFLLAVMLAGGFGLGLVL-PNLTVFAQQAAAREHLGIATALLQSLRM 415 Query: 420 LGQSVGAAL 428 +G +G A+ Sbjct: 416 IGGMLGTAI 424 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 106/409 (25%), Positives = 186/409 (45%), Gaps = 12/409 (2%) Query: 34 LALGIAVGLATLDTAIANTALPAMALDLNTTPAASVWIVTAYQLAMMVALLPLAALGEIV 93 L++ +AV + LD++IAN A+P ++ +L + W++T++ A +++ L + + Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 94 GYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNTALIRFIYPTRSL 153 G R+++ ++LF AS LC +A +LP L+ AR LQGL ++ ++ +L+ YP Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 154 GRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGV--AAVILAL---PSLPH 208 + L + +A GP++ I WPW+F +N+P+G+ A V LA+ P Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPT 206 Query: 209 TALSTHAFDLRSAVLNAAAFGLLILAIGEGAHQAEASVVGLELGAAIVCGLFLLKRELSH 268 L L V AAF +++ + A ++V L + A I F++ EL++ Sbjct: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVV-WELNN 265 Query: 269 PAPMLPVDLFRHPMFTLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPV 328 P++ + LF F I + V LP + LG ++ GL P + Sbjct: 266 DHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 325 Query: 329 AVAIMAPIAGR---LSDRYPVGLLGGVGLALLCAGLLSMTFMPAAPTVWEISWRMLLCGM 385 I+ P+ GR L D V +G A++ TV LL G+ Sbjct: 326 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTV---ILPTLLQGI 382 Query: 386 GFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALVALCFN 434 F P L++ PP R A+G+ + R+ +VG ++ +N Sbjct: 383 PTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,301,340 Number of extensions: 48881 Number of successful extensions: 386 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 369 Number of HSP's successfully gapped: 17 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)