BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0005.0034.p
         (415 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309914|ref|YP_003774006.1| ammonium transporter transmembr...   779   0.0  
gi|300309431|ref|YP_003773523.1| ammonium transporter transmembr...   118   1e-27

>gi|300309914|ref|YP_003774006.1| ammonium transporter transmembrane
           protein [Herbaspirillum seropedicae SmR1]
          Length = 401

 Score =  779 bits (2011), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 392/401 (97%), Positives = 397/401 (99%)

Query: 15  LVDDHKNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYF 74
           +VDDHKNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYF
Sbjct: 1   MVDDHKNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYF 60

Query: 75  FIGYYVAYGVSFFAGAETLAERSGFELVKFFFLLTFAAAIPAIISGGIAERSKFNPQLIA 134
           FIGYYVAY VSFFAGAETLA+RSGFELVKFFFLLTFAAAIPAIISGGIAERSKFNPQL+A
Sbjct: 61  FIGYYVAYRVSFFAGAETLAQRSGFELVKFFFLLTFAAAIPAIISGGIAERSKFNPQLVA 120

Query: 135 TAVLVGLVYPFFEGIAWNQHLGVQAWLAATFGAEFHDFAGSIVVHAVGGWIALPAVLLLG 194
           TAVLVGLVYPFFEGIAWNQHLGVQAWLAATF AEFHDFAGSIVVHAVGGWIALPAVLLLG
Sbjct: 121 TAVLVGLVYPFFEGIAWNQHLGVQAWLAATFDAEFHDFAGSIVVHAVGGWIALPAVLLLG 180

Query: 195 ARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGWFGFNVMSAQTLDKMNGLVAMNSLMAM 254
           ARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGWFGFNVMSAQTLDKMNGLVAMNSLMAM
Sbjct: 181 ARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGWFGFNVMSAQTLDKMNGLVAMNSLMAM 240

Query: 255 AGGTLVALLMGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALVTGGIAGAIFVWMFTRTQ 314
           AGGTLVALLMGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALVTGGIAGAIFVWMFTRTQ
Sbjct: 241 AGGTLVALLMGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALVTGGIAGAIFVWMFTRTQ 300

Query: 315 NKWKIDDVLGVWPLHGLCGLWGGLAAGIFGLQALGGRGGVSFMSQLIGSIMGIAIAAAGG 374
           NKWKIDDVLGVWPLHGLCGLWGGLAAGIFGLQALGGRGGVSF+SQLIGS+MGI IAA GG
Sbjct: 301 NKWKIDDVLGVWPLHGLCGLWGGLAAGIFGLQALGGRGGVSFLSQLIGSVMGIVIAALGG 360

Query: 375 WIVYGLLKKAVGIRLDPEQEFEGADLAIHKISSTAERETSW 415
           WIVYGLLKKAVGIRLDPEQEFEGADLAIHKISSTAERETSW
Sbjct: 361 WIVYGLLKKAVGIRLDPEQEFEGADLAIHKISSTAERETSW 401


>gi|300309431|ref|YP_003773523.1| ammonium transporter transmembrane
           protein [Herbaspirillum seropedicae SmR1]
          Length = 510

 Score =  118 bits (295), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 118/439 (26%), Positives = 193/439 (43%), Gaps = 59/439 (13%)

Query: 20  KNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYFFIGYY 79
             G  A  ++  A +IL    G      G VR KN ++ L++    F++  I++F  GY 
Sbjct: 78  NKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFMIFSLIYILWFVYGYS 137

Query: 80  VAY--GVSFFAGAETL--------AERSGF-------ELVKFFFLLTFAAAIPAIISGGI 122
           +A+  G +FF G + L        +    F       ELV   F   FAA   A+I G  
Sbjct: 138 IAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQGAFAAITVALIIGAF 197

Query: 123 AERSKFNPQLIATAVLVGLVY--------------PFFEGIAWNQHLGVQAWLAATFGAE 168
           AER KF+  ++ + +     Y               F +  A  +   +  WL   F   
Sbjct: 198 AERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEKATAISGWL---FQKG 254

Query: 169 FHDFAGSIVVHAVGGWIALPAVLLLGARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGW 228
             D+AG  VVH       L    ++G R G + KE    A  P ++    +GA +L  GW
Sbjct: 255 ALDYAGGTVVHINAAIAGLVGSYVIGKRIG-FGKE----AFKPHSLTLTMVGASLLWFGW 309

Query: 229 FGFNVMSAQTLDKMNGLVAMNSLMAMAGGTL---VALLMGRNDPGF--AYNGPLAGLVAV 283
           FGFN  S    +    L  +N+++A A   L       +G+  P    A +G +AGLVA+
Sbjct: 310 FGFNAGSGLEANGTAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAI 369

Query: 284 CAGSDLMHPLGALVTGGIAGAIFVWMFTRTQNKWKIDDVLGVWPLHGLCGLWGGLAAGIF 343
              +  +  +GA+V G IAG + +W  +  +     DD L V+ +HG+ G+ G +  G+F
Sbjct: 370 TPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGAILTGVF 429

Query: 344 GLQALGGRGGVSFMS---------------QLIGSIMGIAIAAAGGWIVYGLLKKAVGIR 388
              +LGG G   +++               Q  G +  + ++    +I Y ++   +G+R
Sbjct: 430 AAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIVDVVIGLR 489

Query: 389 LDPEQEFEGADLAIHKISS 407
           +  E+E EG D++ H  S+
Sbjct: 490 VTEEEEREGLDISSHGESA 508


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.142    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,272,399
Number of extensions: 53244
Number of successful extensions: 302
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 295
Number of HSP's successfully gapped: 2
Length of query: 415
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 327
Effective length of database: 1,203,190
Effective search space: 393443130
Effective search space used: 393443130
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)