BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0032.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 130 1e-31 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 125 4e-30 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 85 5e-18 gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 82 3e-17 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 82 5e-17 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/247 (36%), Positives = 128/247 (51%), Gaps = 7/247 (2%) Query: 9 FLITGVSSGFGRALADAALQAGHSVTGTVRSQTAKEQFEQLDPARATAIVLDVTDFPAIA 68 ITG SSGFG +A LQ V T+R T + P + LDV D +I Sbjct: 4 IFITGCSSGFGLEIARYFLQRDWKVIATMR--TPRHDLLPASP-HLRILPLDVADAHSIQ 60 Query: 69 AVAEHLAPVDVLVNNAGYGHEGALEESSLDEMRHQFDVNVFGAVAMMKAVLPTMRARRSG 128 A E PVDVLVNNAG G+ GALE + ++R F+ N G++AM +A +P MRAR G Sbjct: 61 AAVEAAGPVDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRARGQG 120 Query: 129 HIINITSMGGFITMPGIAYYCGSKFALEGISETLAKEVAAFGIKVTAVAPG-SFRTDWAG 187 +IN+TS +P +A Y SK A+ +E+LA E+A G++V V PG S +T +A Sbjct: 121 LVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLPGRSPQTRFAE 180 Query: 188 RSMVRSPRSIAD-YDAVIDPIRAARQAKSGHQLGDPAKAAQVILDIAGDANAPVHLLLGS 246 + R S+ + Y A+ + QA + QL A+ + A D AP+ + G Sbjct: 181 NARARMAASVPEPYAALAAQVFENFQAAT--QLTYAEDVAEAVWQAANDPKAPMRIPAGE 238 Query: 247 DAVKLVR 253 DA + R Sbjct: 239 DAREWAR 245 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 91/252 (36%), Positives = 126/252 (50%), Gaps = 19/252 (7%) Query: 10 LITGVSSGFGRALADAALQAGHSVTGTVRSQTAKEQFEQLDPARATAIV-LDVTDFPAIA 68 LITG SSG+G A L G V T+RS +F+ L P+ I+ LDV+ +I Sbjct: 5 LITGCSSGYGLETARHFLANGWKVIATMRS----PRFDLLSPSDNLCILPLDVSQPDSIK 60 Query: 69 AVAEHLAPVDVLVNNAGYGHEGALEESSLDEMRHQFDVNVFGAVAMMKAVLPTMRARRSG 128 E P+DVLVNNAG G GA E + + +R F+ N FG +AM +AV+P R R G Sbjct: 61 QALEAAGPIDVLVNNAGIGLFGAFEATPMSTVREIFETNTFGVMAMCQAVIPQFRERGDG 120 Query: 129 HIINITSMGGFITMPGIAYYCGSKFALEGISETLAKEVAAFGIKVTAVAPG-----SFRT 183 IIN+TS P +A Y SK A+EG + +LA E+ A G++V V PG SF T Sbjct: 121 VIINVTSSATLAAFPLVAAYTASKTAIEGFTASLAHELKAVGVRVKLVEPGYGPSTSFAT 180 Query: 184 DWAGR--SMVRSPRSIADYDAVIDPIRAARQAKSGHQLGDPAKAAQVILDIAGDANAPVH 241 + R ++ P Y++ + A + + AQ I D A D + +H Sbjct: 181 NGQQRMQGLITEP-----YESFAHEVFAGFGQLT--VVTAATDVAQCIWDAAKDTSDRLH 233 Query: 242 LLLGSDAVKLVR 253 G DAV L + Sbjct: 234 FPAGPDAVALAQ 245 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 85.1 bits (209), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 62/183 (33%), Positives = 85/183 (46%), Gaps = 8/183 (4%) Query: 10 LITGVSSGFGRALADAALQAGHSVTGTVRSQTA----KEQFEQLDPARATAIVLDVTDFP 65 L+TG S G GRA+A + G V GT S++ E L P VL+V D Sbjct: 12 LVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVLNVNDAE 71 Query: 66 AIAAVAEHL----APVDVLVNNAGYGHEGALEESSLDEMRHQFDVNVFGAVAMMKAVLPT 121 A A+ E + + +LVNNAG + +E N+ + +AVL Sbjct: 72 ASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVLRG 131 Query: 122 MRARRSGHIINITSMGGFITMPGIAYYCGSKFALEGISETLAKEVAAFGIKVTAVAPGSF 181 M R+G IINITS+ G PG Y +K + G+S LA+E+ + I V VAPG Sbjct: 132 MMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFI 191 Query: 182 RTD 184 TD Sbjct: 192 DTD 194 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 82.4 bits (202), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 50/150 (33%), Positives = 78/150 (52%), Gaps = 7/150 (4%) Query: 44 EQFEQLDPARATAIVLDVTDFPAIAAVAEHL----APVDVLVNNAGY-GHEGALEESSLD 98 + + P R +I D+TD PA+ A+ L +D L+NNAGY G L+E + Sbjct: 44 QHHDAASPYRQQSI--DITDAPAVMQAAQELLADTGRIDFLINNAGYAGPTVPLDEYDVG 101 Query: 99 EMRHQFDVNVFGAVAMMKAVLPTMRARRSGHIINITSMGGFITMPGIAYYCGSKFALEGI 158 E VN+ G ++V P MRA R G I+NI S+ G P + Y +K + + Sbjct: 102 EWHRIVQVNLLGVFHACRSVTPAMRAARRGRIVNIASLAGKEGTPNASAYSAAKAGVLAL 161 Query: 159 SETLAKEVAAFGIKVTAVAPGSFRTDWAGR 188 +++L KE+A G+ V A+AP + +T G+ Sbjct: 162 TKSLGKELAESGVLVNAIAPAAVKTALLGQ 191 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 82.0 bits (201), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 63/191 (32%), Positives = 95/191 (49%), Gaps = 9/191 (4%) Query: 2 KNSKTLHFLITGVSSGFGRALADAALQAGHS-VTGTVRSQTAKEQFEQLDPA--RATAIV 58 N K +ITG SSG G A G V G R++ ++ + A +A A+ Sbjct: 3 NNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVT 62 Query: 59 LDVT---DFPAIAAVAE-HLAPVDVLVNNAGYGHEGALEESSLDEMRHQFDVNVFGAVAM 114 DV D A+ A E H +DVLVNNAG + + ++E DVNV G + Sbjct: 63 TDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVLYG 122 Query: 115 MKAVLPTMRARRSGHIINITSMGGFITMPGI-AYYCGSKFALEGISETLAKEVAAFGIKV 173 + A LP A+ SGHIIN++S+ G G+ Y +KFA+ ++E L E A G++ Sbjct: 123 VAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTE-APDGVRT 181 Query: 174 TAVAPGSFRTD 184 T ++PG+ ++ Sbjct: 182 TIISPGAVDSE 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 851,777 Number of extensions: 33949 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 45 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)