BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0028.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 332 1e-92 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 112 2e-26 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 107 4e-25 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 99 1e-22 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 99 2e-22 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 332 bits (851), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 164/174 (94%), Positives = 169/174 (97%) Query: 1 VRAVLAGLTQLDIVVNCAGIIKRGEELETDVFEQVIAVNLTGTMRVCAASRALLKQSGGC 60 VRA LAGLTQLDIVVNCAGIIKRG ELET+VFEQVIAVNLTGTMRVCAA+R LL+QS GC Sbjct: 68 VRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAARELLRQSRGC 127 Query: 61 IVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQAL 120 IVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQAL Sbjct: 128 IVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQAL 187 Query: 121 QDDPVRAGPILARTPLGRWGKPEDIGNVVAFLCSPAASFMTGAIVPVDGGYLVA 174 QDDPVRAGPILARTPLGRWG+PED+G VVAFLCSPAASFMTGAIVPVDGGYLVA Sbjct: 188 QDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLVA 241 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 112 bits (279), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 66/165 (40%), Positives = 94/165 (56%), Gaps = 4/165 (2%) Query: 12 DIVVNCAGI--IKRGEELETDVFEQVIAVNLTGTMRVCAA-SRALLKQSGG-CIVNTASM 67 D ++N AGI ++ E+ + +E V+ VNL + C A +R + Q GG +VN S+ Sbjct: 75 DFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNITSI 134 Query: 68 LSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRA 127 G Y+ASK G+ T+ LA GIRVNAVAP T L +A DP ++ Sbjct: 135 AGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDPAKS 194 Query: 128 GPILARTPLGRWGKPEDIGNVVAFLCSPAASFMTGAIVPVDGGYL 172 GP++AR P R+ PED+ +A L SP A+ + GA+VPVDGG+L Sbjct: 195 GPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFL 239 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 107 bits (268), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 60/167 (35%), Positives = 91/167 (54%), Gaps = 2/167 (1%) Query: 10 QLDIVVNCAGIIKRGE--ELETDVFEQVIAVNLTGTMRVCAASRALLKQSGGCIVNTASM 67 QLD++V+ AG+ G E+ + + +V A N+ G + L+++ GCIVN AS+ Sbjct: 89 QLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREALPHLEKTRGCIVNVASV 148 Query: 68 LSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRA 127 + Y+ASKG V LT+++A+ + G+R+NAV P T +T + DDP Sbjct: 149 SGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMTADMADDPPLL 208 Query: 128 GPILARTPLGRWGKPEDIGNVVAFLCSPAASFMTGAIVPVDGGYLVA 174 R LGR P +I V+ FL SP ASF+ G +PVDGG + + Sbjct: 209 DKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGLMAS 255 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 99.4 bits (246), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 63/163 (38%), Positives = 96/163 (58%), Gaps = 5/163 (3%) Query: 11 LDIVVNCAGIIKR--GEELETDVFEQVIAVNLTGTMRVCAAS-RALLKQSGGCIVNTASM 67 L I+VN AGI + ++ + ++ VIA NLT R+ A R ++K G I+N S+ Sbjct: 87 LSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVLRGMMKARTGRIINITSV 146 Query: 68 LSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRA 127 + G Y+A+K GVA ++++LA + I VN VAPG+I T +T+AL + ++ Sbjct: 147 VGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRALNEQ--QS 204 Query: 128 GPILARTPLGRWGKPEDIGNVVAFLCSPAASFMTGAIVPVDGG 170 IL + PLGR G E+I VAFL SP A+++TG + V+GG Sbjct: 205 AAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGG 247 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 99.0 bits (245), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 59/173 (34%), Positives = 92/173 (53%), Gaps = 3/173 (1%) Query: 1 VRAVLAGLTQLDIVVNCAGIIKRG--EELETDVFEQVIAVNLTGTMRVCAA-SRALLKQS 57 V+ + + + + I+VN AGI +R EE + + +I+ NL A +R ++ + Sbjct: 87 VQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRG 146 Query: 58 GGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLT 117 G I+N S+ S G + Y+A+KG V TK +AI + GI+VN + PG+ T + Sbjct: 147 AGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMN 206 Query: 118 QALQDDPVRAGPILARTPLGRWGKPEDIGNVVAFLCSPAASFMTGAIVPVDGG 170 AL +D ++ RTP RWG ED+ FL S A+ F+ G I+ VDGG Sbjct: 207 MALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGG 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 573,639 Number of extensions: 24143 Number of successful extensions: 169 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 133 Number of HSP's successfully gapped: 37 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)