BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0026.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 832 0.0 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 196 5e-51 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 165 7e-42 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 121 1e-28 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 121 1e-28 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 832 bits (2148), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/428 (97%), Positives = 425/428 (99%) Query: 1 MEVQKEKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 MEVQKEKSPRLKRIQ+VALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA Sbjct: 1 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 Query: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEA 120 FSLAYAFSQLPIGAMLDKFGARVMLGAGMF+WS+AQLAGGFV LQQFLVARVFLGLGEA Sbjct: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 Query: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLL 180 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMF+IMG+L Sbjct: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 Query: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDQDPNANAKLSWAEWKSLFTYRTTWGML 240 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHD DPNANAKLSW+EWKSLFTYRTTWGML Sbjct: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML 240 Query: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR Sbjct: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 Query: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA Sbjct: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 Query: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV 420 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV Sbjct: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV 420 Query: 421 DKPIEAHA 428 DKPIE+HA Sbjct: 421 DKPIESHA 428 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 196 bits (497), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 129/424 (30%), Positives = 218/424 (51%), Gaps = 17/424 (4%) Query: 13 RIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPI 72 ++ + L L + VNY DR+TLSIA ++ EL+L MG + SAFS AY +QLP Sbjct: 10 HVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPG 69 Query: 73 GAMLDKFGARVMLGAGMFVWSLAQ-LAGGFVQTLQQFLVARVFL-----GLGEAPQFPAG 126 G +LD+FG++ + + +WS+ L G VA +FL GL EAP FPA Sbjct: 70 GWLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPAN 129 Query: 127 AKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVSV 186 ++V+ WF +ERG + IF ++ L P++ ++ ++ W +F +MG++GIA+++ Sbjct: 130 GRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALAL 189 Query: 187 GW-YLFYRNRNEVQLTPSEVLY------LADDHDQDPNANAKLSWAEWKSLFTYRTTWGM 239 W + Y ++ ++ +E+ + L D ++ A + + L + R G+ Sbjct: 190 IWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGV 249 Query: 240 LFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMK 299 G + + + +LTW P YL ER +SI + G+V IP + G +G + G I+D L+K Sbjct: 250 YIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIK 309 Query: 300 RGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGG-AWALVS 358 RG+ +RK PI G++ I Y + M + +A+AF G WA+V+ Sbjct: 310 RGLSVTWARKIPIVGGML-LSMTMILCNYVEAQWMVVGI--MALAFFGKGVGALGWAVVA 366 Query: 359 VAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMF 418 P+++V G + N G + G P++ G +V +T SF AL+ A + Y+ Sbjct: 367 DTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALVFVGINALVTVISYLV 426 Query: 419 VVDK 422 +V + Sbjct: 427 IVKE 430 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 165 bits (417), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 112/388 (28%), Positives = 188/388 (48%), Gaps = 10/388 (2%) Query: 11 LKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQL 70 ++ I+ ++ + LA IVNYL R+TLS+A ++ +EL++S Q ++ A+ + YA Q Sbjct: 4 VRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQP 63 Query: 71 PIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEAPQFPAGAKVV 130 G +LD G ++ G WSL A F Q R LG+ EA P G K Sbjct: 64 VAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKAS 123 Query: 131 SEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVSVGWYL 190 +EWF +ER G F S+IG APP++ +L GW+M F+++G LG+ V W L Sbjct: 124 TEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWML 183 Query: 191 FYRN-RNEVQLTPSEVLYLADDHDQDPNANAKLSWAEWKSLFTYRTTWGMLFGFMGVIYM 249 FY++ R++ L+P E Y+ + ++ P + SW + + R W + Sbjct: 184 FYKSPRDQKLLSPEERAYILEGQEKSPEKVQRESWTK---IVRSRNFWSIAIPRFLSEPA 240 Query: 250 VWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSR 308 + W+P Y+ ERH++I +P++ +G + GY++ K + SR Sbjct: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 Query: 309 KWPICAGLIGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLV 366 K L+G+ + PA + S +AI +S+ SG +++ S + V Sbjct: 301 K---LVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQV 357 Query: 367 ASLGSMQNFGGYLGGSLAPVITGVVVDQ 394 A+ + GYLG +L ++ G++V Q Sbjct: 358 ATATGLTGMSGYLGATLFTLLFGILVTQ 385 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 121 bits (304), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 100/333 (30%), Positives = 154/333 (46%), Gaps = 40/333 (12%) Query: 3 VQKEKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFS 62 +Q K R + AL F++ V DR+ L +A + +E +S ++ G L S F Sbjct: 6 IQHTKKKTHFRWVVGALIFIVYT--VAAADRANLGVALPFLRKEFVMSNTEAGALASIFL 63 Query: 63 LAYAFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAG----GFVQTLQQFLVARVF---L 115 AY+ +QLP G +FG V+SLA LA G + T L ++F L Sbjct: 64 FAYSLAQLPSGLAYTRFGIPR-------VFSLAMLATSVFTGLIGTASSLLALKIFRFGL 116 Query: 116 GLGEAPQFPAG-AKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMF 174 GL E P P G ++ WF RE+G TGIF+A++ GP L PP+ ++M +GWR +F Sbjct: 117 GLAEGP-LPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIF 175 Query: 175 IIMGLLGIAVSVGWYLFYRNR--NEVQLTPSEVLYLADD------HDQDPNAN------- 219 + G+ +SV W + +NR ++ E+ ++ D D DP Sbjct: 176 YFFAVPGVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLD 235 Query: 220 -----AKLSWAE-WKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTG 273 +K++ E K +F G G+ + +V + L W+P YL + SIA G Sbjct: 236 RIIRRSKVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMG 295 Query: 274 WVVVIPYIFGTLGMLSSGYIADG-LMKRGMPAI 305 V P++ +G L G + D L KR PA+ Sbjct: 296 MVAAAPWVGAVIGNLLGGAMTDRWLEKRRKPAM 328 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 87/319 (27%), Positives = 147/319 (46%), Gaps = 29/319 (9%) Query: 22 LMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDKFGA 81 + L I NYL R++L + ++ EL +S Q +++AF + Y Q G ++D G Sbjct: 15 VCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFIVDLIGL 74 Query: 82 RVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRERGG 141 R+ +WS+A + R +GL EA P+G K V+EWF +E+ Sbjct: 75 RIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSV 134 Query: 142 PTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVSVGWYLFYRN-RNEVQL 200 G F + +++G LAPP++ L + +GW+ F++ G+LG + W+ FYR+ R+ L Sbjct: 135 AVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRDHAAL 194 Query: 201 TPSEVLYLADDHDQDPNA-----------NAKLSWAEWKSLFTYRTTWGMLFGFMGVIYM 249 + SE + + +A ++ WA + F W F F Sbjct: 195 SDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEPAW-QTFSF------ 247 Query: 250 VWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSR 308 W+P Y +ERHL + +P++ LG L+ GY++ L+ R +P + SR Sbjct: 248 ------WIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWSR 301 Query: 309 KWPICAGLIGAGAFTIPAA 327 +C ++GA PA Sbjct: 302 ---VCGVVLGAVMMIGPAC 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,315,235 Number of extensions: 55974 Number of successful extensions: 387 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 352 Number of HSP's successfully gapped: 30 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)