BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0025.p (240 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 424 e-120 gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 220 7e-59 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 124 6e-30 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 124 1e-29 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 119 2e-28 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 424 bits (1090), Expect = e-120, Method: Compositional matrix adjust. Identities = 223/244 (91%), Positives = 228/244 (93%), Gaps = 7/244 (2%) Query: 4 MTYSRASHDFGGCVAVVTGGHGGIGTAISARLAAGGAQVVVWDQQHDAASPYRQESLDIT 63 MTYSRAS+DFG CVAVVTGGHGGIG AISARLAAGGAQVVVWDQ HDAASPYRQ+S+DIT Sbjct: 1 MTYSRASYDFGDCVAVVTGGHGGIGAAISARLAAGGAQVVVWDQHHDAASPYRQQSIDIT 60 Query: 64 DAAAVMQAAQRLLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFYL--- 120 DA AVMQAAQ LLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVF+ Sbjct: 61 DAPAVMQAAQELLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFHACRS 120 Query: 121 ----XRAAGRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAETGVLVNAIA 176 RAA RGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAE+GVLVNAIA Sbjct: 121 VTPAMRAARRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAESGVLVNAIA 180 Query: 177 PAAVKTALLEQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDLSGG 236 PAAVKTALL QMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDLSGG Sbjct: 181 PAAVKTALLGQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDLSGG 240 Query: 237 RATY 240 RATY Sbjct: 241 RATY 244 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 220 bits (561), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 121/247 (48%), Positives = 155/247 (62%), Gaps = 17/247 (6%) Query: 11 HDFGGCVAVVTGGHGGIGTAISARLAAGGAQVVVWDQQHDAASPYRQ--ESL-------- 60 +DF G A++TGG G G A++ RL G A+VV+WD A + R+ ESL Sbjct: 4 YDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVETVKV 63 Query: 61 DITDAAAVMQAAQRLLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFYL 120 DI+ A V QA + T ID L+++AG AG P+ EY EW R++ ++L G FY+ Sbjct: 64 DISSQAEVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYV 123 Query: 121 XR-------AAGRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAETGVLVN 173 + A GRIVNIAS+AGKEG P ASAYSAAKAG++ALTKSLGKE A + VN Sbjct: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 Query: 174 AIAPAAVKTALLEQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDL 233 AI PAA +T +LEQ + AH+ M++K P R V+E+A M AWL S SF T +VFDL Sbjct: 184 AITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASVFDL 243 Query: 234 SGGRATY 240 SGGRATY Sbjct: 244 SGGRATY 250 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 124 bits (312), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 76/241 (31%), Positives = 127/241 (52%), Gaps = 24/241 (9%) Query: 17 VAVVTGGHGGIGTAISARLAAGGAQVVVW-----DQQHDAASPYRQESLDI-------TD 64 +A VTGG GGIGTAI RL G VV ++ + R++ DI ++ Sbjct: 5 IAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVSN 64 Query: 65 AAAVMQAAQRLLADTGRIDFLINNAGYA--GPTVPLDEYDVGEWHRIVQVNLLGVFYLXR 122 + A +++ A+ G +D L+NNAG G + + D W ++ NL +F + + Sbjct: 65 WESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDD---WDAVIDTNLNSLFNVTK 121 Query: 123 AA-------GRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAETGVLVNAI 175 G GRI+NI+S+ G++G + YS AKAG+ T +L +E+A GV VN + Sbjct: 122 QVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTV 181 Query: 176 APAAVKTALLEQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDLSG 235 +P + T ++ + P ++ +++ P+ RLG +E+A + AW+ S + TG+ F L+G Sbjct: 182 SPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNG 241 Query: 236 G 236 G Sbjct: 242 G 242 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 87/245 (35%), Positives = 126/245 (51%), Gaps = 20/245 (8%) Query: 11 HDFGGCVAVVTGGHGGIGTAISARLAAGGAQVVVWDQQHDAASP---------YRQESL- 60 +D G VA+VTGG GIG +IS +LAA G V+V D DAAS +R L Sbjct: 4 NDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQ 63 Query: 61 -DITDAAAVMQAAQRLLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFY 119 D+ ++ A + D GR D L+NNAG A LD Y + W + + VN+ GV Sbjct: 64 MDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLD-YPLENWLQTMNVNVTGVLL 122 Query: 120 LXRAAGR-------GRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAETGVLV 172 + A R GRIVNI+S++G +AY +KA V+ LT+ + ELA+ G+ V Sbjct: 123 AGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITV 182 Query: 173 NAIAPAAVKTALLEQMSPAHVQTMIAK-SPMHRLGSVDEVADMCAWLCSGSCSFNTGAVF 231 N++AP V T + + M + + PM R G+ DE+A +L + ++ TG V Sbjct: 183 NSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVI 242 Query: 232 DLSGG 236 + GG Sbjct: 243 PVDGG 247 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 119 bits (298), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 82/251 (32%), Positives = 127/251 (50%), Gaps = 28/251 (11%) Query: 9 ASHDFGGCVAVVTGGHGGIGTAISARLAAGGAQVVVWDQQHDAASPY------------R 56 ++ + G VA+VTG GIG AI+ LA GA+V+ A+ R Sbjct: 2 SAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGR 61 Query: 57 QESLDITDAAAVMQAAQRLLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLG 116 L++ DA A + +++ D G + L+NNAG + + D EW ++ NL Sbjct: 62 GAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDE-EWDSVIATNLTA 120 Query: 117 VFYLXRAAGRG-------RIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAETG 169 V L RA RG RI+NI S+ G+ G P Y+AAKAGV ++++L +E+ Sbjct: 121 VGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN 180 Query: 170 VLVNAIAPAAVKT----ALLEQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSF 225 + VN +AP + T AL EQ S A +Q + P+ RLG+ +E+A A+L S ++ Sbjct: 181 ITVNCVAPGFIDTDMTRALNEQQSAAILQQI----PLGRLGAAEEIAYAVAFLASPQAAY 236 Query: 226 NTGAVFDLSGG 236 TG +++GG Sbjct: 237 ITGTTLNVNGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 790,433 Number of extensions: 33045 Number of successful extensions: 319 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 46 Length of query: 240 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 157 Effective length of database: 1,226,865 Effective search space: 192617805 Effective search space used: 192617805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)