BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0020.p (137 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309923|ref|YP_003774015.1| membrane protein [Herbaspirillu... 229 6e-62 >gi|300309923|ref|YP_003774015.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 138 Score = 229 bits (584), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 130/137 (94%), Positives = 135/137 (98%) Query: 1 MTTVTVNTRADYAAFLLRVTMGIFFLVHAGLKLFVFTPAGTVGFFASLGVPGWFAYPVMA 60 M+TVTVNTRADYAAFLLRVTMG+FFLVHAGLK+FVFTPAGTVGFFASLGVPGWFAYPV+A Sbjct: 1 MSTVTVNTRADYAAFLLRVTMGVFFLVHAGLKIFVFTPAGTVGFFASLGVPGWFAYPVIA 60 Query: 61 LEVVGGIALVLGFYGRWLALPLAADLLGAIVLVHGKAGFFFTNPKGGWEYLALWIVGLIA 120 LEVVGGIALVLG+YGRWLALPLAADLLGAIVLVHGKAGFFFTNPKGGWEYLALWIVGLIA Sbjct: 61 LEVVGGIALVLGYYGRWLALPLAADLLGAIVLVHGKAGFFFTNPKGGWEYLALWIVGLIA 120 Query: 121 VFLLGDGAFALKREAQR 137 VFLLGDGAFALKRE R Sbjct: 121 VFLLGDGAFALKREEPR 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.148 0.475 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 428,682 Number of extensions: 16670 Number of successful extensions: 83 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 83 Number of HSP's successfully gapped: 1 Length of query: 137 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 61 Effective length of database: 1,260,010 Effective search space: 76860610 Effective search space used: 76860610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)