BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0016.p (141 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309926|ref|YP_003774018.1| inner membrane protein [Herbasp... 268 1e-73 >gi|300309926|ref|YP_003774018.1| inner membrane protein [Herbaspirillum seropedicae SmR1] Length = 141 Score = 268 bits (685), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 133/141 (94%), Positives = 137/141 (97%) Query: 1 MLWIKALHIVFIASWFAGLFYLPRIFVNLAQETDLVARERLLGMARRLYRFTGMISLVAL 60 MLWIKALHIVFIASWFAGLFYLPRIFVNLAQET++ +RERLLGMARRLYRFTGMISLVAL Sbjct: 1 MLWIKALHIVFIASWFAGLFYLPRIFVNLAQETEVASRERLLGMARRLYRFTGMISLVAL 60 Query: 61 VLGVWLLAITWSFWKGPGNGWLHAKLFLVVLVIGYQHACGSLLKKFEHGKNVRSHVWYRW 120 LGVWLL +TW FWKGPGNGWLHAKLFLVVLVIGYQHACGSLLKKFEHGKNVRSHVWYRW Sbjct: 61 ALGVWLLVLTWQFWKGPGNGWLHAKLFLVVLVIGYQHACGSLLKKFEHGKNVRSHVWYRW 120 Query: 121 FNEVPVLLMLAIVILVVVKPF 141 FNEVPVLLMLAIVILVVVKPF Sbjct: 121 FNEVPVLLMLAIVILVVVKPF 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.334 0.146 0.499 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 393,430 Number of extensions: 13171 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 61 Number of HSP's successfully gapped: 1 Length of query: 141 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 65 Effective length of database: 1,260,010 Effective search space: 81900650 Effective search space used: 81900650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 126 (53.1 bits)