BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0012.p (882 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbasp... 1814 0.0 gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 85 3e-17 >gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 882 Score = 1814 bits (4699), Expect = 0.0, Method: Compositional matrix adjust. Identities = 867/882 (98%), Positives = 877/882 (99%) Query: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKEGRDKKKFYACSMLPYPSGKLHMGHVR 60 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDK+GRDKKKFYACSMLPYPSGKLHMGHVR Sbjct: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKQGRDKKKFYACSMLPYPSGKLHMGHVR 60 Query: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNIEYMKSQMQS 120 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNI+YMKSQMQS Sbjct: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNIDYMKSQMQS 120 Query: 121 MGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG 180 MGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG Sbjct: 121 MGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG 180 Query: 181 RGWRSGAVIEKREIPMYYAKITDYADELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA 240 RGWRSGAVIEKREIPMYYAKITDYADELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA Sbjct: 181 RGWRSGAVIEKREIPMYYAKITDYADELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA 240 Query: 241 FPHQIKGADGQLINDGKLFVFTTRADTIMGVTFCAVAPEHPLATLAAENNPALQAFIAEC 300 FPHQIKGADGQLINDGKLFVFTTRADTIMGVTFCAVAPEHPLATLAAEN+PALQAFIAEC Sbjct: 241 FPHQIKGADGQLINDGKLFVFTTRADTIMGVTFCAVAPEHPLATLAAENDPALQAFIAEC 300 Query: 301 KQGSVIEADMATMEKKGMPTGLFVTHPLTGEQVEVWVGNYVLITYGDGAVMGVPAHDERD 360 KQGSVIEADMATMEKKGMPTGLFVTHPLTG+QVEVWVGNYVLITYGDGAVMGVPAHDERD Sbjct: 301 KQGSVIEADMATMEKKGMPTGLFVTHPLTGDQVEVWVGNYVLITYGDGAVMGVPAHDERD 360 Query: 361 FAFARKYNLPIKQVIAVEGKTYSTEAWEEWYGDKEAGKTIASGKYDGLGYQAAVDAVAAD 420 FAFARKYNLPIKQVI VEGKTYSTEAWEEWYGDKEAGKTI SGKYDGLGYQAAVDAVAAD Sbjct: 361 FAFARKYNLPIKQVIGVEGKTYSTEAWEEWYGDKEAGKTIHSGKYDGLGYQAAVDAVAAD 420 Query: 421 LAAQGLGEKKVTFRLRDWGISRQRYWGTPIPIIHCPDCGDVPVPEKDLPVVLPEDCVPDG 480 LAAQGLGEKKVTFRLRDWGISRQRYWGTPIPIIHCPDCGDVPVPEKDLPVVLPEDCVPDG Sbjct: 421 LAAQGLGEKKVTFRLRDWGISRQRYWGTPIPIIHCPDCGDVPVPEKDLPVVLPEDCVPDG 480 Query: 481 SGNPLNKHEQFLKVDCPCCGKPARRETDTMDTFVDSSWYYMRYTSPGSNEAMVDSRNDYW 540 SGNPLNKHEQFLKVDCPCCGKPARRETDTMDTFVDSSWYYMRYTSPGSNEAMVDSRNDYW Sbjct: 481 SGNPLNKHEQFLKVDCPCCGKPARRETDTMDTFVDSSWYYMRYTSPGSNEAMVDSRNDYW 540 Query: 541 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG 600 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG Sbjct: 541 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG 600 Query: 601 KKTWYNPADVDLSFDDKGRPVSAVLKEDGQPVMIGGTEKMSKSKNNGIDPQAQIDQYGAD 660 KKTWYNPADVDLSFDDKGRPVSAVLKEDGQPVMIGGTEKMSKSKNNGIDPQAQIDQYGAD Sbjct: 601 KKTWYNPADVDLSFDDKGRPVSAVLKEDGQPVMIGGTEKMSKSKNNGIDPQAQIDQYGAD 660 Query: 661 TARLFTMFASPPEQTLEWSGAGVEGANRFLRRVWAFGYNNAARITAAGALDAAQLDDGLK 720 TARLFTMFASPPEQTLEWSGAGVEGANRFLRRVWAFGYNNAARI+AAGA+DAAQLDD LK Sbjct: 661 TARLFTMFASPPEQTLEWSGAGVEGANRFLRRVWAFGYNNAARISAAGAVDAAQLDDSLK 720 Query: 721 TLRREVHKVLQQADNDFKRIQYNTVVSACMKMLNTLEAAKPAAGAAGDAVIAECFSIFLR 780 TLRREVHKVLQQADNDFKRIQYNTVVSACMKMLNTLEAAKPAAGAAGDAVIAECFSIFLR Sbjct: 721 TLRREVHKVLQQADNDFKRIQYNTVVSACMKMLNTLEAAKPAAGAAGDAVIAECFSIFLR 780 Query: 781 VLNPVAPHITHVLWQQLGYAQAQGDILDAAWPQVDAAALEQSELKLVVQVNGKLRGEVTV 840 VLNPVAPHITHVLWQ+LGYA+AQGDILDA WPQVDAAALEQSE+KLVVQVNGKLRGEVTV Sbjct: 781 VLNPVAPHITHVLWQELGYAKAQGDILDADWPQVDAAALEQSEIKLVVQVNGKLRGEVTV 840 Query: 841 PKDADKAAIEAAALANEDVKKFLTGAPKKVIVVPGKLVNIVA 882 KDADKAAIEAAALANEDV+KFLTGAPKKVIVVPGKLVNIVA Sbjct: 841 SKDADKAAIEAAALANEDVRKFLTGAPKKVIVVPGKLVNIVA 882 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 84.7 bits (208), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 58/203 (28%), Positives = 90/203 (44%), Gaps = 26/203 (12%) Query: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKEGRDKKKFYACSMLPYPSGKLHMGHVR 60 + + PAE+E+ ++ W + +A +K F P +G LHMGH Sbjct: 38 LAKSFEPAEIEKFWREEWEKRGYFAATTDA------EKPSFSIQLPPPNVTGTLHMGHAF 91 Query: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPA----ENAAMANGVPPA------------ 104 N TI D + RY RM GYN G D G+ E A V Sbjct: 92 NQTIMDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVW 151 Query: 105 QWTYSNIEYMKSQMQSMGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 +W + + QM+ MG + DW+RE P+ K +F+++ E+G+IY+ VNW Sbjct: 152 EWKEKSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNW 211 Query: 165 DPIDQTVLANEQVI----DGRGW 183 DP+ T +++ +V+ DG W Sbjct: 212 DPVLGTAVSDLEVVSEEEDGSMW 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,753,145 Number of extensions: 116835 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 320 Number of HSP's successfully gapped: 2 Length of query: 882 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 788 Effective length of database: 1,174,780 Effective search space: 925726640 Effective search space used: 925726640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)