BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0176.p (356 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 641 0.0 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 145 5e-36 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 115 5e-27 gi|300309866|ref|YP_003773958.1| major facilitator superfamily p... 110 1e-25 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 108 9e-25 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 641 bits (1654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/333 (95%), Positives = 327/333 (98%) Query: 1 MSTGAAVIALFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVIFT 60 MST AAV +LFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAV+FT Sbjct: 102 MSTAAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFT 161 Query: 61 PLMAWIVHTYSWHHVFTVMGVVGILLAMVWGKIIYSPKDHPRINPAELEHIESGGGLVDM 120 PLMAWIVHTYSWHHVFTVMGVVGI LA++WG+IIYSPKDHPRIN AEL HIESGGGLVDM Sbjct: 162 PLMAWIVHTYSWHHVFTVMGVVGIALALIWGRIIYSPKDHPRINQAELAHIESGGGLVDM 221 Query: 121 DNKKVAAPSTKGKGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILK 180 DN+KVAAPSTKG+GYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILK Sbjct: 222 DNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILK 281 Query: 181 AGFVASIPAVCGFIGGVVGGLISDRLIKSGMSVTWARKIPIVGGMLLSMTMILCNYVDAQ 240 AGFVASIPAVCGFIGGVVGGLISDRLIK G+SVTWARKIPIVGGMLLSMTMILCNYV+AQ Sbjct: 282 AGFVASIPAVCGFIGGVVGGLISDRLIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEAQ 341 Query: 241 WMVVGIMALAFFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVK 300 WMVVGIMALAFFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVK Sbjct: 342 WMVVGIMALAFFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVK 401 Query: 301 ETGSFAGALVFVGINALVTVISYLVIVKEIKRV 333 ETGSF+GALVFVGINALVTVISYLVIVKEIKRV Sbjct: 402 ETGSFSGALVFVGINALVTVISYLVIVKEIKRV 434 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 145 bits (366), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 91/319 (28%), Positives = 162/319 (50%), Gaps = 11/319 (3%) Query: 14 RFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVIFTPLMAWIVHTYSWH 73 R +GL EAP FPA ++V+ WF +ERG + IF ++ + P++ ++ ++ W Sbjct: 112 RVFLGLGEAPQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWR 171 Query: 74 HVFTVMGVVGILLAMVWGKIIYSPKDHPRINPAELEHIESGGGLVDMDNKKVAAPSTKGK 133 +F +MGV+GI +++ W + Y ++ ++ P+E+ ++ D DN A Sbjct: 172 MMFVIMGVLGIAVSVGW-YLFYRNRNEVQLTPSEVLYLAD-----DHDNDPNANAKLSWS 225 Query: 134 GYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGF 193 + + L + R G+ G + + + +LTW P YL ER +SI + G+V IP + G Sbjct: 226 EW-KSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGT 284 Query: 194 IGGVVGGLISDRLIKSGMSVTWARKIPIVGGMLLSMTMIL-CNYVDAQWMVVGI--MALA 250 +G + G I+D L+K GM +RK PI G++ + + Y + M + +A+A Sbjct: 285 LGMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMA 344 Query: 251 FFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFAGALV 310 F G WA+V+ P+++V G + N G + G P++ G +V +T SF AL+ Sbjct: 345 FVNMASGG-AWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALL 403 Query: 311 FVGINALVTVISYLVIVKE 329 A + Y+ +V + Sbjct: 404 ISAAVAFAAALVYMFVVDK 422 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 115 bits (288), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 92/356 (25%), Positives = 170/356 (47%), Gaps = 44/356 (12%) Query: 3 TGAAVIALFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVIFTPL 62 T ++++AL + RF +GLAE P + WFP +E+GT + IF +A F V+ P+ Sbjct: 102 TASSLLALKIFRFGLGLAEGPLPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPV 161 Query: 63 MAWIVHTYSWHHVFTVMGVVGILLAMVWGKIIYS-PKDHPRINPAELEHIESGGG----- 116 A I++ + W H+F V G++L++VW ++ + P + P ++ ELEHI G Sbjct: 162 CAVIMYYWGWRHIFYFFAVPGVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQ 221 Query: 117 -----------LVD--MDNKKVAA-PSTKGKGYIRQLLSNRMLLGVYIGQYCINVITYFF 162 L+D + KVA +TKG + + ++G +G + + I Sbjct: 222 DADPAVTRDFALLDRIIRRSKVATIETTKG------VFCSWNIMGAALGYFFMTAIVNVL 275 Query: 163 LTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIKSGMSVTWARKIPIV 222 L W P YL+ +G SI G VA+ P V IG ++GG ++DR ++ R+ P Sbjct: 276 LAWIPTYLISVKGYSIANMGMVAAAPWVGAVIGNLLGGAMTDRWLEK-------RRKP-- 326 Query: 223 GGMLLS------MTMILCNYVDAQWMVVGIMALAFFGKGVGALGWAVV-ADTAPKQIVGL 275 M++S M +IL N + ++ L +G + V K++ + Sbjct: 327 -AMMMSAFFTTIMMVILINSPADPLLYGALLLLTGILLNIGYSTYMVYPMGMTSKKVYPM 385 Query: 276 SGGLFNMFGNVAGITTPIVIGYIVKETGSFAGALVFVGINALVTVISYLVIVKEIK 331 + + N G + G P+ G+++ ++ S+ +F+ + +++ I+ L I + ++ Sbjct: 386 ACAMVNTLGQLGGACAPLAAGFLL-DSYSWNHVWIFMAVCSILCFITILTIREPVQ 440 >gi|300309866|ref|YP_003773958.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 484 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 89/331 (26%), Positives = 155/331 (46%), Gaps = 43/331 (12%) Query: 1 MSTGAAVIALFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVIFT 60 M T ++V+ L L R +GL+E P ++ WFP +E+GTA+ ++ +A +I Sbjct: 130 MGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTATGLWIAASKLGPLIVP 189 Query: 61 PLMAWIVHTYSWHHVFTVMGVVGILLAMVWGKIIY-SPKDHPRINPAELEHIESG----- 114 + ++ + W +F V V GI LA++W ++ SP ++ N AE +I Sbjct: 190 TVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCNEAERRYITQDVAAGP 249 Query: 115 -----------GGLV------DMDNKKVAAPSTKGKGYIRQLLSNRMLLGVYIGQYCINV 157 G V D N+ P + +RQ+ ++ ++GV IG C+ Sbjct: 250 ATATASSRAHVGAQVTPMPWLDGINRTRRVPRLE---TLRQVFTSWNVIGVAIGYGCMIG 306 Query: 158 ITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIKSGMSVTWAR 217 I+ F++W P YLV +G + +K GF+AS P + G ++GG+ISDRL+ R Sbjct: 307 ISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDRLLG-------GR 359 Query: 218 KIPIVGGMLLS------MTMILCNYVDAQWMVVGIMALAFFGKGVGALGWAVV-ADTAPK 270 + P+ MLL MT++L D+ + ++ L+ G+G G++ A K Sbjct: 360 RKPM---MLLGALGTALMTLLLVEAPDSVLYLGAMLMLSGLMLGIGFAGYSAYPMGLATK 416 Query: 271 QIVGLSGGLFNMFGNVAGITTPIVIGYIVKE 301 + G+ N G + G P+ +G ++ Sbjct: 417 ATYPAAFGIVNSLGQIGGACAPLAVGMLLDR 447 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 108 bits (269), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 74/293 (25%), Positives = 138/293 (47%), Gaps = 10/293 (3%) Query: 13 LRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVIFTPLMAWIVHTYSW 72 R ++GL EA + P+ + VA WFP KE+ A FNS A++ PL+ ++ Y W Sbjct: 104 FRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGW 163 Query: 73 HHVFTVMGVVGILLAMVWGKIIYSPKDHPRINPAELEHIESGGGLVDMDNKKVAAPSTKG 132 F V G++G + A +W SP+DH ++ +E E I SG + V+A +G Sbjct: 164 QSAFVVTGILGFVWAALWFAFYRSPRDHAALSDSERETIISG------QLRPVSA--VRG 215 Query: 133 KGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCG 192 K ++ ++ +R + + ++ F W P+Y V ER + + A +P + Sbjct: 216 KRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAA 275 Query: 193 FIGGVVGGLISDRLI-KSGMSVTWARKIPIVGGMLLSMTMILCNYVDAQWMVVGIMALAF 251 +GG+ GG +S LI + + + W+R +V G ++ + V + ++ + + + Sbjct: 276 DLGGLAGGYLSPFLINRFRIPLVWSRVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGG 335 Query: 252 FG-KGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETG 303 F + + L + AD + VG + G M + G+ +++G + G Sbjct: 336 FAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVG 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,159,861 Number of extensions: 52289 Number of successful extensions: 332 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 16 Length of query: 356 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 270 Effective length of database: 1,212,660 Effective search space: 327418200 Effective search space used: 327418200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)