BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0175.p (521 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herb... 1021 0.0 gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspiri... 265 5e-72 >gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 1021 bits (2641), Expect = 0.0, Method: Compositional matrix adjust. Identities = 499/522 (95%), Positives = 506/522 (96%), Gaps = 1/522 (0%) Query: 1 MSTDTAASQAFTPRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTLVDRVPQGHKIALR-V 59 MSTDT+ASQA PRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTLVDRVPQGHKIALR + Sbjct: 1 MSTDTSASQASAPRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTLVDRVPQGHKIALRDL 60 Query: 60 SSWTFXGFFDVAIGYAVRDIPKGSWIEESLVQMPPARGLDNLPIATKKPAPQPPLDGYTF 119 +DVAIGYAVRDIPKG WIEESLVQMPPAR LDNLPIATKKPAPQPPL+GYTF Sbjct: 61 KQGEAIVRYDVAIGYAVRDIPKGGWIEESLVQMPPARELDNLPIATKKPAPQPPLEGYTF 120 Query: 120 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG 179 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG Sbjct: 121 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG 180 Query: 180 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKAGEPYVV 239 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHK GEPYVV Sbjct: 181 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKQGEPYVV 240 Query: 240 CLQDAEHVGFNSMIDSIMKMAEARLTELDKRRRETCPASDLVVGVQCGGSDAFSGVTANP 299 CLQDAEHVGFNSMIDSIM MAEARLTEL+KRRRETCPASDLVVGVQCGGSDAFSGVTANP Sbjct: 241 CLQDAEHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTANP 300 Query: 300 AVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQGGV 359 AVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQGGV Sbjct: 301 AVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQGGV 360 Query: 360 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLAPGDKLQQKGLIYAATPASDFI 419 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVL+PGDKLQQKGLIYAATPASDFI Sbjct: 361 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLSPGDKLQQKGLIYAATPASDFI 420 Query: 420 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEAT 479 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEA+ Sbjct: 421 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEAS 480 Query: 480 IEQVGWELFQLMLDVASGKKRTWAEQWKLHNALTLFNPAPVT 521 IE VGWELFQLMLDVASGKKRTWAEQWKLHNALTLFNPAPVT Sbjct: 481 IEDVGWELFQLMLDVASGKKRTWAEQWKLHNALTLFNPAPVT 522 >gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspirillum seropedicae SmR1] Length = 511 Score = 265 bits (678), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 179/509 (35%), Positives = 265/509 (52%), Gaps = 39/509 (7%) Query: 9 QAFTPRYIMMNDTDNVAIVVNDGGLPAGTVFPD--GLTLVDRVPQGHKIALR-VSSWTFX 65 +A +P I +N D+V I L +GTV D GL + +P GHK+A R +++ Sbjct: 2 KAHSP-VIRLNPVDDVVIARQQ--LISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPV 58 Query: 66 GFFDVAIGYAVRDIPKGSWIEESLVQMPPAR-----GLDNLPIA-TKKPAPQPPLDGYTF 119 + IG A +DI G + + M G D P+ PA TF Sbjct: 59 KRYGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPA--------TF 110 Query: 120 EGYRNADGSVGTRNLLAITTTVQCVA----GVVEHAVKRIRAELLPKYPNVEDVVALEHT 175 G DG V TRN + + T+V C A + ++ + I E+L YPN++ VVAL H Sbjct: 111 MGIVRPDGRVATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHG 170 Query: 176 YGCGVAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKAGE 235 GC + I RTL + +PNF +VV LGCE Q + L+ + + K GE Sbjct: 171 QGCALDSQGEPLQILRRTLAGYATHPNFAA-VLVVGLGCETNQISGLMESHNL---KEGE 226 Query: 236 PY-VVCLQ----DAEHVGFNSMIDSIMKMAEARLTELDKRRRETCPASDLVVGVQCGGSD 290 + +Q A+ V I+ I KM L + + +RE A L +G+QCGGSD Sbjct: 227 YFHTFTIQGTGGTAKTVALG--IEKIKKM----LPKANDIKREPVSAKHLTLGLQCGGSD 280 Query: 291 AFSGVTANPAVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWY 350 +SG+TANPA+G A DLLVR G + + SE E+ LT RAV+ EV + ++ + W+ Sbjct: 281 GYSGITANPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWW 340 Query: 351 DNYLKQGGVDRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLAPGDKLQQKGLIY 410 + Y + + + N + GNK GGL I+EK++G++ K G++ + V + + +G ++ Sbjct: 341 EEYCAKNDAEMNNNPSAGNKAGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVF 400 Query: 411 AATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNA 470 TP D I T Q+A G N+ FTTGRG+ YG A P +K+AT L +R + MD+N Sbjct: 401 MDTPGYDPISATGQVAGGANMICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINC 460 Query: 471 GRIASGEATIEQVGWELFQLMLDVASGKK 499 G IA G T +++G FQ++LD ASGKK Sbjct: 461 GEIADGNVTPQEIGERFFQMILDTASGKK 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,757,351 Number of extensions: 78690 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 2 Length of query: 521 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 431 Effective length of database: 1,193,720 Effective search space: 514493320 Effective search space used: 514493320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)