BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0004.0164.p
         (208 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...   374   e-105
gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba...    72   3e-14
gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...    63   1e-11

>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score =  374 bits (961), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 198/208 (95%), Positives = 205/208 (98%)

Query: 1   MPDLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFA 60
           MPDL LFLLAAMTLTLAPGPDN+YVLTRGIAQGRKAGLVAAAGFCSGL+FHTLLAVLGFA
Sbjct: 1   MPDLILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFA 60

Query: 61  ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRRIYWQSVLA 120
           ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSAS+G+AL+ADGQGAAAVPLRRIYWQSVLA
Sbjct: 61  ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASAGLALAADGQGAAAVPLRRIYWQSVLA 120

Query: 121 NMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAVFR 180
           NMLNPKVTLFFIAFLPQFVQREAG+EALQML+LALVFIVQAF IFSAIALFSGAVGA FR
Sbjct: 121 NMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSGAVGAYFR 180

Query: 181 RRASASVHLNRLAGCAFIGLGIRMALPE 208
           RRASASVHLNRLAGCAFIGLGIRMALPE
Sbjct: 181 RRASASVHLNRLAGCAFIGLGIRMALPE 208


>gi|300314064|ref|YP_003778156.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 210

 Score = 72.0 bits (175), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 66/207 (31%), Positives = 92/207 (44%), Gaps = 10/207 (4%)

Query: 1   MPDLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFA 60
           + D++  + A M   L PGP    VLT     G + G  A AG   G +    LA  G A
Sbjct: 10  ITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAAGVA 69

Query: 61  ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRRI--YWQSV 118
           AL+ A P  +H LQY GAAYLAYLG R L +   G        G   VP      + +  
Sbjct: 70  ALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEG--------GGTVVPFSNAAEFRRGF 121

Query: 119 LANMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAV 178
           L  + NPK  +F++AF P F+       AL  + +  V     F+  S + +   A    
Sbjct: 122 LVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAARR 181

Query: 179 FRRRASASVHLNRLAGCAFIGLGIRMA 205
             R  + +   +R AG   IG GI++ 
Sbjct: 182 MARNRTIARLASRAAGVFLIGFGIKLT 208


>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score = 63.2 bits (152), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 56/198 (28%), Positives = 89/198 (44%), Gaps = 11/198 (5%)

Query: 5   FLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFAALIK 64
           F F  AA  + ++PG   +  ++ G+  G +       G    L+   ++A  G  +L+ 
Sbjct: 7   FAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAGVGSLLV 66

Query: 65  AYPPAYHALQYAGAAYLAYLGVRTLR----SASSGIALSADGQGAAAVPLRRIYWQSV-- 118
           A   A++ ++ AGA YL YLG    R    S   G AL A    +A    R+ +WQ    
Sbjct: 67  ASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANA-SARAHARQTWWQRCMT 125

Query: 119 --LANMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVG 176
             L N  NPK  +F +A LPQF+  +     LQ+L+LA+  +    V+    A  +  + 
Sbjct: 126 GFLTNATNPKGIVFMVAVLPQFIDHDRPLW-LQLLVLAVTMVAVDLVVMHGYAFAASRLQ 184

Query: 177 AVFRRRASASVHLNRLAG 194
             FR + +     NR  G
Sbjct: 185 RFFRSQKAIRAQ-NRFFG 201


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.331    0.142    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 534,036
Number of extensions: 18525
Number of successful extensions: 123
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 120
Number of HSP's successfully gapped: 4
Length of query: 208
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 127
Effective length of database: 1,236,335
Effective search space: 157014545
Effective search space used: 157014545
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 128 (53.9 bits)