BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0151.p (366 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor his... 704 0.0 gi|300309416|ref|YP_003773508.1| two component sensor histidine ... 84 1e-17 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 67 2e-12 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 66 4e-12 gi|300313917|ref|YP_003778009.1| two component sensor histidine ... 66 5e-12 >gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor histidine kinase) transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 366 Score = 704 bits (1817), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/366 (95%), Positives = 356/366 (97%) Query: 1 MTPGIKNPPMKTPLPSLDGLDLLASAVIILDANGGIVYANAAAENLLESSFKVLSQQKLS 60 M PG MKTP PSL+GLDLLASAVIILDA GGIVYANAAAENLLESSFKVLSQQKLS Sbjct: 1 MNPGSPTTAMKTPFPSLEGLDLLASAVIILDAEGGIVYANAAAENLLESSFKVLSQQKLS 60 Query: 61 ELFLNGKELTALFYQAIEHQFADKRLDLVLERANREPLQVHTIVTPLDSTDWPVLLELRE 120 ELFLNGKEL+ALFYQAIEHQFADKRLDLVLERANREPLQVHTIVTPLD+ D PVLLELRE Sbjct: 61 ELFLNGKELSALFYQAIEHQFADKRLDLVLERANREPLQVHTIVTPLDNPDMPVLLELRE 120 Query: 121 NVQQLKLDREERMFDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREY 180 NVQQLKLDREER+FDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREY Sbjct: 121 NVQQLKLDREERIFDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREY 180 Query: 181 TQVIIKEADRLQTLVDRLLAPHRHPHIVGDVNIHEVCERVRSLVMAEFPQGLSIKRDYDL 240 TQVIIKEADRLQTLVDRLLAPHRHPHIVGDVNIHEVCERVRSLVMAEFPQGL+IKRDYDL Sbjct: 181 TQVIIKEADRLQTLVDRLLAPHRHPHIVGDVNIHEVCERVRSLVMAEFPQGLTIKRDYDL 240 Query: 241 SIPEFRGDKEQLIQAVLNIVHNAAQALADRIRAGDGELILRTRVSRQVTLSKVRYRLALD 300 SIPEFRGDKEQLIQAVLNIVHNAAQALADRIRAGDGELILRTRVSRQVTLSKVRYRLALD Sbjct: 241 SIPEFRGDKEQLIQAVLNIVHNAAQALADRIRAGDGELILRTRVSRQVTLSKVRYRLALD 300 Query: 301 LHIIDNGPGIPPDIQERIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFR 360 LHIIDNGPGIPPDIQ+RIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFR Sbjct: 301 LHIIDNGPGIPPDIQDRIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFR 360 Query: 361 ILIPLP 366 ILIPLP Sbjct: 361 ILIPLP 366 >gi|300309416|ref|YP_003773508.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 760 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 73/240 (30%), Positives = 122/240 (50%), Gaps = 18/240 (7%) Query: 136 QSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKE----LREYTQVIIKEADRL 191 +S A E+ R LAHEIKNPL I+ +A+ L+ L ++ + L + T I+ + + Sbjct: 522 RSIAWGEVARRLAHEIKNPLTPIQLSAERLQHRLSDKLMPADAAILDKGTTTIVNQVTAM 581 Query: 192 QTLVDRLLAPHRHPH---IVGDVNIHEVCERVRSLVMAEFPQGLSIKRDYDLSIPEFRGD 248 + +VD R P + D+N + E + +L +A + +I S+ + GD Sbjct: 582 KRMVDDFRDYARTPPAKPVALDLN--ALIEEILNLYLAGDGRD-AIHARLAPSMAKVMGD 638 Query: 249 KEQLIQAVLNIVHNAAQALADRIRAGDGELILRTRV-SRQVTLSKV--RYRLALDLHIID 305 QL Q + N++ NA A+AD +GE+ R V + Q+ ++ + + A+ L I+D Sbjct: 639 ATQLRQVIHNLLQNAQDAVAD-----EGEVAPRIDVLTEQIDIAGADGQRQPAVRLSIMD 693 Query: 306 NGPGIPPDIQERIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFRILIPL 365 NGPG I R F P V+ + G+GLGL + + V +H G I+ +R +I+I L Sbjct: 694 NGPGFSAKILARAFEPYVTSKSKGTGLGLAMVKKIVEEHGGRIDIQNRTDGRGAKIVILL 753 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 66/237 (27%), Positives = 107/237 (45%), Gaps = 14/237 (5%) Query: 138 QANKELIRNLAHEIKNPLGGIRGAAQLLELELPERH--LKELREYTQVIIKEADRLQTLV 195 +A +L +AH+ N L I G ++ L L L + I K + + L Sbjct: 174 EALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLT 233 Query: 196 DRLLAPHRHPHIVG-DVNIHEVCERVRSLVMAEFPQGLSIKRDYDLSIPEFRGDKEQLIQ 254 +LLA R + G +N++ + E LV ++++ + + R D Q+ Sbjct: 234 QQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPTQMEV 293 Query: 255 AVLNIVHNAAQALADR----IRAGDGELILRTRVSRQVTLSKVRYRLALDLHIIDNGPGI 310 A+LN++ NA A+ R IR + +L R V L ++ L + + + D+GPGI Sbjct: 294 ALLNVLINARDAMNGRGRVRIRTANEDL---ADPDRAVQLG-LKEGLYVSIAVTDDGPGI 349 Query: 311 PPDIQERIFYPLVSGR-EG-GSGLGLTLAQTFVHQHMGVIECDSRP-GCTDFRILIP 364 PDI R+ P + + EG G+GLGL++ FV Q G + S G T I P Sbjct: 350 APDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFP 406 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 66.2 bits (160), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 71/250 (28%), Positives = 108/250 (43%), Gaps = 43/250 (17%) Query: 138 QANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIKEADRLQTLVDR 197 +A +L LAH+ N LGGI G+ L+ + L + +EL Y ++ + R +L R Sbjct: 466 EAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRGEELLRYVELAARSTQRAASLTQR 525 Query: 198 LLAPHRHPHIVGDVNIHEVCERVRSLV--MAEFPQGLSIKRDYDLSI-----------PE 244 LLA R + RV +L+ M E I+R +I P Sbjct: 526 LLAFARRQTLAPKPT------RVNALISGMEEL-----IRRTIGPAIVLEVVGAGGLWPT 574 Query: 245 FRGDKEQLIQAVLNIVHNAAQALADRIRAGDGELILRT-------RVSRQVTLSKVRYRL 297 F D QL A+LN+ NA A+ G G L + T R + + L +Y Sbjct: 575 FV-DGPQLESALLNLCINARDAM-----PGGGRLTIETANKWLDDRWAHERDLPPGQY-- 626 Query: 298 ALDLHIIDNGPGIPPDIQERIFYPLVSGRE--GGSGLGLTLAQTFVHQHMGVIECDSRPG 355 + + + D+G G+PPD+ R F P + + G+GLGL++ F Q G + S G Sbjct: 627 -VCISVTDSGVGMPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVG 685 Query: 356 -CTDFRILIP 364 T I +P Sbjct: 686 QGTTVSIYLP 695 >gi|300313917|ref|YP_003778009.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 517 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 61/223 (27%), Positives = 104/223 (46%), Gaps = 18/223 (8%) Query: 146 NLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIKEADRLQTLVDRLLA---PH 202 +AHEI+NP+G +R A+ P+ ++++ + +RL LV LLA P Sbjct: 305 GMAHEIRNPIGTMRMKAENALAAPPQMREARATSALEMVVAQTERLDALVGSLLALSQPF 364 Query: 203 RHPHIVGDVNIHEVCERVRSLVMAEFPQGLSIKRDYDLSIPEFRGDKEQLIQAVLNIVHN 262 + D+ R+ L MAE QG+ I+ +++PE ++ L+ L+ Sbjct: 365 QLQRQPLDLAQWLPQRRLAHLEMAER-QGVRIQ----VTLPE----QDDLVTHSLDPAQ- 414 Query: 263 AAQALADRIRAGDGELILRTRVSRQVTL-SKVRYRLALDLHIIDNGPGIPPDIQERIFYP 321 LA + + ++ + +++R L L + DNGPG+P D+++ +F P Sbjct: 415 ----LARALDNLLLNALAHCPAGGEIEIGARLRSPGRLFLWVADNGPGVPQDLRQSLFDP 470 Query: 322 LVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFRILIP 364 VS R GG+GLGL L + +H H G I F + +P Sbjct: 471 FVSQRPGGTGLGLVLVREIIHAHGGEIILAPTAAGARFEMELP 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,052,779 Number of extensions: 40538 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 21 Length of query: 366 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 280 Effective length of database: 1,212,660 Effective search space: 339544800 Effective search space used: 339544800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)