BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0148.p (531 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312424|ref|YP_003776516.1| methyl-accepting chemotaxis sen... 655 0.0 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 358 e-100 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 339 3e-94 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 339 4e-94 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 338 7e-94 >gi|300312424|ref|YP_003776516.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 531 Score = 655 bits (1690), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/517 (70%), Positives = 437/517 (84%) Query: 1 MLGFSVLILFMAVLGAAAWYGLNTVDAQLNEVQTNLMPSVATAKTMQAEIRAFNLAQYRA 60 MLGFS+LILFMA LG + Y + ++AQL +VQ N MPSV TA+TM AE+RA NLAQYRA Sbjct: 1 MLGFSILILFMAALGGTSLYIMGKINAQLVDVQGNWMPSVTTAQTMLAEMRAVNLAQYRA 60 Query: 61 LTANDAQRMQDARTRLTASLGRYTSAADQYRPLIHTPEEQAAYDELQALLPKYLAVSQQM 120 LTA++AQRMQDARTR+TA+LGRYT A++QYR LI +P EQ+AYDELQALL +Y+ + Q+ Sbjct: 61 LTADNAQRMQDARTRITAALGRYTKASEQYRHLISSPAEQSAYDELQALLARYIQIGDQL 120 Query: 121 LAALEQGKQEQAVDIMKDQLFPLRAAMEVQILRLITVNEKGVAEAATSAREAYAGSRMLI 180 ++EQGK+E+A + MKD L+P+R AME +I ++I +N++G A+A + +YA S + I Sbjct: 121 PQSVEQGKEEEAANTMKDTLYPVRTAMEAKIQQIIKINQEGAAQAVEHGQASYAQSHLTI 180 Query: 181 IGLTLASALIGLLIALLISRRLTRQLGGEPAEAMWLSAQIAAGNLTAQLHLRKDDRASLM 240 I TLA+AL GL IALLISRRLTRQLGGEP EAMWLS QIAAGNL A+L L+ +D SLM Sbjct: 181 IAFTLAAALAGLGIALLISRRLTRQLGGEPGEAMWLSGQIAAGNLAARLELKSNDNGSLM 240 Query: 241 HSLMTMREQLAGLVGQIQQASGAIHVAAREIAQGNTDLSQRTEEQAASLEETASSMEQLT 300 SLM MR+QL+GLVGQIQ AS +IHVAA+EIAQGN DLSQRTEEQAASLEETASSME+LT Sbjct: 241 RSLMIMRDQLSGLVGQIQHASTSIHVAAQEIAQGNIDLSQRTEEQAASLEETASSMEELT 300 Query: 301 STVQQNTDHARKASHVASAGADVARRGETEIAQVVSTMREIAVSSHQMSDIISVIEGIAF 360 STVQQN +A++AS +ASAGA+VARRGE EIA VV+TMRE++VSSHQMSDIISVIEGI+F Sbjct: 301 STVQQNAGNAKQASSMASAGAEVARRGEAEIADVVATMREMSVSSHQMSDIISVIEGISF 360 Query: 361 QTNILALNAAVEAARAGENGRGFAVVATEVRALAQRSAGAAKEIKGLIEGSVGRIQSGTT 420 QTNILALNAAVEAARAGENGRGFAVVA+EVRALAQRS+ AAKEIK LIE SVGRI++G+ Sbjct: 361 QTNILALNAAVEAARAGENGRGFAVVASEVRALAQRSSAAAKEIKDLIEDSVGRIRNGSQ 420 Query: 421 LVEKAGGTIAEVMRASVDTTRIISDIAHASEEQSAGITQINAAILQMDQVTQQNAALVEQ 480 LVE AG TIAE+M++SV TT ++ +IA ASEEQSAGI Q+N AI+QMDQVTQQNAALVE+ Sbjct: 421 LVEAAGATIAEIMQSSVRTTDLMKEIAAASEEQSAGIGQVNTAIVQMDQVTQQNAALVEE 480 Query: 481 AAAAAQAMMQQAEQLTSAASVFRIDAQLLQAAPLTPA 517 AAAAAQ+MMQQA+ LT+A SVF++D Q L+ P PA Sbjct: 481 AAAAAQSMMQQAQALTAAVSVFKLDDQALRTLPGMPA 517 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 229/518 (44%), Positives = 340/518 (65%), Gaps = 13/518 (2%) Query: 4 FSVLILFMAVLGAAAWYGLNTVDAQLNEVQTNLMPSVATAKTMQ---AEIRAFNLAQYRA 60 F V++ +A LG + L+ V++ E+ TN +PS+ T +Q + +R+F L Sbjct: 17 FLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQLAMSRLRSFELQH--- 73 Query: 61 LTANDAQRMQDARTRLTASLGRYTSAADQYRPLIHTPEEQAAYDELQALLPKYLAVSQQM 120 + A DA+ ++ + + +A +Y I PEE+A Y E++ L+ +++ ++ Sbjct: 74 ILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVEKLIATFVSEHDKI 133 Query: 121 LAALEQGKQEQAVDIMK-DQLFPLRAAMEVQILRLITVNEKGVAEAATSAREAYAGSRML 179 +A QGK E+A ++ + + R +++ QI +L VN+KG + A YA +R+ Sbjct: 134 IALSRQGKDEEARNLQRGESTKAYRTSLQ-QIDKLAAVNDKGADISNKQADAIYAQARLW 192 Query: 180 IIGLTLASALIGLLIALLISRRLTRQLGGEPAEAMWLSAQIAAGNLTAQLH-LRKDDRAS 238 II A + +L+A+ +SR + R L EA+ ++ ++A G+LT ++ KD+ Sbjct: 193 IIVTVAACIGLAMLLAVWVSRLIARPL----EEAVSVAQRVAGGDLTTRIEPAGKDETGQ 248 Query: 239 LMHSLMTMREQLAGLVGQIQQASGAIHVAAREIAQGNTDLSQRTEEQAASLEETASSMEQ 298 L+ +L +M + L G+VGQ++ + I A+ EIA GN DLS RTEEQA+SLEETAS+ME+ Sbjct: 249 LLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLEETASAMEE 308 Query: 299 LTSTVQQNTDHARKASHVASAGADVARRGETEIAQVVSTMREIAVSSHQMSDIISVIEGI 358 LTSTV+QN D+AR+A+ +A + ++VA +G + QVV TM I SS +++DII VI+GI Sbjct: 309 LTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIADIIGVIDGI 368 Query: 359 AFQTNILALNAAVEAARAGENGRGFAVVATEVRALAQRSAGAAKEIKGLIEGSVGRIQSG 418 AFQTNILALNAAVEAARAGE GRGFAVVA+EVR+LAQRSAGAAKEIK LIEGSV ++ G Sbjct: 369 AFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEGSVAQVDMG 428 Query: 419 TTLVEKAGGTIAEVMRASVDTTRIISDIAHASEEQSAGITQINAAILQMDQVTQQNAALV 478 + LVE+AG T++EV+++ T I+ +I+ AS+EQS GI Q+N AI QMD+VTQQNAALV Sbjct: 429 SKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIAQMDEVTQQNAALV 488 Query: 479 EQAAAAAQAMMQQAEQLTSAASVFRIDAQLLQAAPLTP 516 E+AAAAAQ++ +QAE LT F+++ Q + A P Sbjct: 489 EEAAAAAQSLQEQAETLTQTVGFFKLEQQPDEPASRPP 526 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 339 bits (870), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 215/506 (42%), Positives = 328/506 (64%), Gaps = 7/506 (1%) Query: 1 MLGFSVLILFMAVLGAAAWYGLNTVDAQLNEVQTNLMPSVATAKTMQAEIRAFNLAQYRA 60 ++ F+ +I+ +LG A L V+ ++ TN +P++ A+++QA + +++ + Sbjct: 14 IVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASLPRMRISELQM 73 Query: 61 LTANDAQRMQDARTRLTASLGRYTSAADQYRPLIHTPEEQAAYDELQALLPKYLAVSQQM 120 +TA +DA + + L Y I EE+ Y++ YL + +++ Sbjct: 74 VTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFSKSFATYLEIDKEL 133 Query: 121 LAALEQGKQEQAVDIMK-DQLFPLRAAMEVQILRLITVNEKGVAEAATSAREAYAGSRML 179 +GK+E+A + K D R+ +E + +I VN+ G + A + Y + + Sbjct: 134 ARISAEGKEEEARALFKGDSNKTFRSMIE-NMDEIIKVNDAGSKRSDDMAEQVYRSATLW 192 Query: 180 IIGLTLASALIGLLIALLISRRLTRQLGGEPAEAMWLSAQIAAGNLTAQLH-LRKDDRAS 238 IIGL A +I L +A++++R ++R L EA+ ++ ++A G+LT + D+ Sbjct: 193 IIGLLAAIVVIALGLAVMVARAVSRPLN----EAVAVAERVAQGDLTMDIRATSTDETGM 248 Query: 239 LMHSLMTMREQLAGLVGQIQQASGAIHVAAREIAQGNTDLSQRTEEQAASLEETASSMEQ 298 LMHSL M + L +VG+++ + I A++EIA GN DLS RTEEQA SLEETAS+ME+ Sbjct: 249 LMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLEETASAMEE 308 Query: 299 LTSTVQQNTDHARKASHVASAGADVARRGETEIAQVVSTMREIAVSSHQMSDIISVIEGI 358 LT+TV+QN D+AR+A+ +A++ +D+A +G + QVV TM I SS++++DIISVI+GI Sbjct: 309 LTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIADIISVIDGI 368 Query: 359 AFQTNILALNAAVEAARAGENGRGFAVVATEVRALAQRSAGAAKEIKGLIEGSVGRIQSG 418 AFQTNILALNAAVEAARAGE GRGFAVVA+EVR+LAQRSA AAKEIK LI+ SV ++ +G Sbjct: 369 AFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKVGAG 428 Query: 419 TTLVEKAGGTIAEVMRASVDTTRIISDIAHASEEQSAGITQINAAILQMDQVTQQNAALV 478 + LVE+AG T++EV+ + T ++++I+ AS EQS GI +IN AI QMD+VTQQNAALV Sbjct: 429 SQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQMDEVTQQNAALV 488 Query: 479 EQAAAAAQAMMQQAEQLTSAASVFRI 504 E+AAAAAQ++ QA +L+ SVF++ Sbjct: 489 EEAAAAAQSLQDQAARLSQVVSVFKL 514 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 339 bits (869), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 226/521 (43%), Positives = 324/521 (62%), Gaps = 31/521 (5%) Query: 2 LGFSVLILFMAVLGAAAWYGLNTVDAQLNE-VQTNLMPSVATAKTMQAEIRAF---NLAQ 57 LGF+ +++ + V+ +G+ TV L++ V N +A IRA N+A Sbjct: 12 LGFASVVVLLVVISLVGLWGMATVKGNLSDIVANNQQGGLADVMNNSVRIRALAIRNIAL 71 Query: 58 YR--ALTANDAQRM-------QDARTRLTASLGRYTSAADQYRPLIHTPEEQAAYDELQA 108 ++ AL A + +R+ QDAR +L G+ D R E +D + Sbjct: 72 FQEPALIAREIERIKVQEKAYQDAREKL----GKMFDQPDTTR------REHELFDLIAQ 121 Query: 109 LLPKYLAVSQQMLAALEQGKQEQAVDIMKDQLFPLRAAMEVQILRL----ITVNEKGVAE 164 + Q + +Q K + + ++ ++ P++A + L +N + A Sbjct: 122 NAADTEPLMNQAVTLAQQDKSAELISLLTQKIRPVQAKWLDNLDELGRFEDELNNQASAA 181 Query: 165 AATSAREAYAGSRMLIIGLTLASALIGLLIALLISRRLTRQLGGEPAEAMWLSAQIAAGN 224 A T+ R + L+ G S L+ + A LI R + QLGGEPA+A L+AQIA GN Sbjct: 182 ADTTYRSILTTTWTLVAG----SVLLAVAAAFLIVRSVLHQLGGEPAQAQELAAQIAQGN 237 Query: 225 LTAQLHLRKDDRASLMHSLMTMREQLAGLVGQIQQASGAIHVAAREIAQGNTDLSQRTEE 284 L + L K D+ SLM SL MR QL +V I+ ++ +I AA +IA GN DLS+RTEE Sbjct: 238 LAVSIPLAKGDQHSLMASLENMRAQLTRIVDGIKHSAESISTAASQIAVGNLDLSRRTEE 297 Query: 285 QAASLEETASSMEQLTSTVQQNTDHARKASHVASAGADVARRGETEIAQVVSTMREIAVS 344 QAASLEETASSME++TSTV+QNT +A + +A + + A++G + +VV TM++I+ S Sbjct: 298 QAASLEETASSMEEITSTVEQNTQNAGQGHQLARSASLHAQKGGEVVERVVGTMKDISQS 357 Query: 345 SHQMSDIISVIEGIAFQTNILALNAAVEAARAGENGRGFAVVATEVRALAQRSAGAAKEI 404 ++++++II+ +EGIAFQTNILALNAAVEAARAGE GRGFAVVATEVR+LAQRSA +AKEI Sbjct: 358 AYKITEIIATVEGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASSAKEI 417 Query: 405 KGLIEGSVGRIQSGTTLVEKAGGTIAEVMRASVDTTRIISDIAHASEEQSAGITQINAAI 464 +GLIE SV +++G LV++AG T+ EV+R+ ++S+I AS EQS+GI+QIN AI Sbjct: 418 RGLIESSVQHVEAGQKLVDEAGLTMDEVVRSIQQVNDLMSEITAASNEQSSGISQINIAI 477 Query: 465 LQMDQVTQQNAALVEQAAAAAQAMMQQAEQLTSAASVFRID 505 QMD+VTQQNAALVE++A+AAQ+M QQA L A +VFRI+ Sbjct: 478 SQMDEVTQQNAALVEESASAAQSMAQQANSLRDAVAVFRIE 518 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 338 bits (867), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 244/545 (44%), Positives = 333/545 (61%), Gaps = 27/545 (4%) Query: 2 LGFSVLILFMAVLGAAAWYGLNTVDAQL------NEVQTNLMPSVATAKTMQA-EIRAFN 54 LGF ++++F+ + A YG+ V+ L N + L+ ++ A + +A IR Sbjct: 12 LGFGLILVFLLSISALGLYGMARVNDALTDITDVNNAEIKLVTALRNALSQRALAIRNIA 71 Query: 55 LAQYRALTANDAQ---RMQDARTRLTASLGRY--TSAADQYRP---LIHTPEEQAAYDEL 106 L +A ++Q R + A+L + T AA R L+ ++AA Sbjct: 72 LLDEQADMQVESQTLARQEKLYADAYAALDKMFTTEAATTERERQLLVQIKNDEAAT--- 128 Query: 107 QALLPKYLAVSQQMLAALEQGKQEQAVDIMKDQLFPLRAAMEVQILRLITVNEKGVAEAA 166 LP Q L + K +A ++ Q+ P + A + L ++ EAA Sbjct: 129 ---LPLMAKAVQLGL----ENKAAEAGKMLITQVRPKQQAWIKSLTELAEFEDQLNDEAA 181 Query: 167 TSAREAYAGSRMLIIGLTLASALIGLLIALLISRRLTRQLGGEPAEAMWLSAQIAAGNLT 226 TSAR YA RM+ + LA+ L+G+ + LI+R + RQLG EP+EA ++ IA+G+LT Sbjct: 182 TSARNTYAWLRMVTVAAALAALLVGVAASALIARSILRQLGAEPSEAQAVARDIASGDLT 241 Query: 227 AQLHLRKDDRASLMHSLMTMREQLAGLVGQIQQASGAIHVAAREIAQGNTDLSQRTEEQA 286 A + +R+ D +SLM S+ MR QL + I+ A+ I VA+ +IAQGN DLSQRTEEQA Sbjct: 242 ATVRVREGDSSSLMASIEQMRYQLNVIAHGIKSAAETISVASGQIAQGNFDLSQRTEEQA 301 Query: 287 ASLEETASSMEQLTSTVQQNTDHARKASHVASAGADVARRGETEIAQVVSTMREIAVSSH 346 ASLEETA+SME+LT+TV+QNT HA +A ++ + A G +VV TM I SS Sbjct: 302 ASLEETAASMEELTTTVRQNTQHASEALRLSGGATETAAAGSQAFDRVVQTMDRITASSS 361 Query: 347 QMSDIISVIEGIAFQTNILALNAAVEAARAGENGRGFAVVATEVRALAQRSAGAAKEIKG 406 +MSDIISVIEGIAFQTNILALNAAVEAARAGE GRGFAVVA+EVR+LAQRSA AAKEIK Sbjct: 362 KMSDIISVIEGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAKEIKE 421 Query: 407 LIEGSVGRIQSGTTLVEKAGGTIAEVMRASVDTTRIISDIAHASEEQSAGITQINAAILQ 466 LI S+ + +G+ LV AG +A+++ + I+++IA A++EQ +GI QIN A++Q Sbjct: 422 LIGESMSHVGAGSQLVSDAGQQMAQIVASVQRFGEIMNNIATATQEQGSGIEQINTAMVQ 481 Query: 467 MDQVTQQNAALVEQAAAAAQAMMQQAEQLTSAASVFRIDAQLLQAAPLT-PALT-TNGQP 524 MDQVTQQNAALVE+A+AAAQ++ QQA L SVF+I AAPL ALT T G P Sbjct: 482 MDQVTQQNAALVEEASAAAQSLSQQASGLLKVVSVFKIRQDRSLAAPLEQAALTRTPGMP 541 Query: 525 HMPRL 529 MP L Sbjct: 542 GMPAL 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.125 0.325 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,139,395 Number of extensions: 38529 Number of successful extensions: 584 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 71 Length of query: 531 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 441 Effective length of database: 1,193,720 Effective search space: 526430520 Effective search space used: 526430520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)