BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0147.p (427 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 825 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 387 e-109 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 366 e-102 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 300 2e-82 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 294 8e-81 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 825 bits (2130), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/427 (97%), Positives = 424/427 (99%) Query: 1 MTSNTAAPGAGTTHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 M+SNTAAPGA THPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL Sbjct: 1 MSSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 Query: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYATIGLLA 120 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYA+IGLLA Sbjct: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA 120 Query: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAAIFGAVLN 180 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAA+FGAVLN Sbjct: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTDAPLRDTFAHQKM 240 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKT+APLRDTFAHQKM Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 Query: 241 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGAMSD 300 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGA+SD Sbjct: 241 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 Query: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFASMPALLADI 360 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFA+MPALLADI Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 Query: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGNKLSVSYFVIFGAVLSLIATLAA 420 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTG+KLSVSYFVIFGAVLSLIA+L+A Sbjct: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA 420 Query: 421 RFVLRLR 427 R VLRLR Sbjct: 421 RLVLRLR 427 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 387 bits (995), Expect = e-109, Method: Compositional matrix adjust. Identities = 201/413 (48%), Positives = 278/413 (67%), Gaps = 5/413 (1%) Query: 13 THPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFM 72 ++ Q RRAI+++ IGN LEWFD +Y FAV+IAK FFPTG+D SLLL ATFGV FFM Sbjct: 15 SNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 73 RPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYATIGLLAPAGIALGKMIQG 132 RP+G ++LG Y+DR GRKAAL ++I+LM VGT MI P+Y +IGL AP I + +++QG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 133 FSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAAIFGAVLNNMLTPEQLASW 191 FSAGGE GS+TAFL E+AP R R ++SSW AS G+++LL A G + L LASW Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 192 GWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTDAPLRDTFAHQKMRLLIGAGSVIM 251 GWR+PF+ G++I P G YIR HL+E P F E +KTD+PL++ + VI+ Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVIL 254 Query: 252 ATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGAMSDKIGRTPFMIT 310 TV Y+ L Y+PTY+VK L LP + F A V GL + + +P+VG ++D+IGR P + Sbjct: 255 WTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSG 314 Query: 311 SSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFASMPALLADIFPVATRGTGM 370 S+AL ++L YPMF ++ ++P LL+ Q V G L+ Y + A +++FP TG+ Sbjct: 315 SAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGL 374 Query: 371 SLAYNIAVTIFGGFAGLIITWLIDFTGNKLSVSYFVIFGAVLSLIATLAARFV 423 S+AYN+AVTIFGGFA ITWLI TG+ ++ +++V+ A +S + T RFV Sbjct: 375 SVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT---RFV 424 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 366 bits (940), Expect = e-102, Method: Compositional matrix adjust. Identities = 181/415 (43%), Positives = 280/415 (67%), Gaps = 7/415 (1%) Query: 14 HPQ--ARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFF 71 PQ +R +I++ +GNALEW+D ++YG F+ +IA+ FFP ++ SLL+ ATFG+ FF Sbjct: 2 QPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFF 61 Query: 72 MRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYATIGLLAPAGIALGKMIQ 131 MRP+G V+LG Y+DR GRKAA+ + I+LMT+ A+IAF P+YATIG+ AP I + +M+Q Sbjct: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 Query: 132 GFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAAIFGAVLNNMLTPEQLAS 190 GF+ GGE+ SSTAFLVE AP H+RG + SWQ+ Q +++ A GA++ L+PE L S Sbjct: 122 GFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDS 181 Query: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTD-APLR--DTFAHQKMRLLIGAG 247 WGWR+PF+ GLLI P G++IRRH+ E +F ES+ K + AP++ D +L+ G Sbjct: 182 WGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMG 241 Query: 248 SVIMATVSVYLSLY-IPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGAMSDKIGRTP 306 VI TV+ Y+ L +PT+A KQLGLP +F +A ++ P GA+SD++GR Sbjct: 242 QVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRT 301 Query: 307 FMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFASMPALLADIFPVATR 366 ++ SS ++++ YP+FV+++ +P +LL++Q ++ +++ F MP L++ FP R Sbjct: 302 VLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVR 361 Query: 367 GTGMSLAYNIAVTIFGGFAGLIITWLIDFTGNKLSVSYFVIFGAVLSLIATLAAR 421 TG+++AYN+A +FGGFA I+TWL +G+ ++ +++V+F A + + A A R Sbjct: 362 STGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMR 416 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 300 bits (768), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 169/422 (40%), Positives = 253/422 (59%), Gaps = 12/422 (2%) Query: 1 MTSNTAAPGAGTTHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 MT++TA+ +T ++ + GN +E FD ++G +A I+K FFP+ ++ SL+ Sbjct: 1 MTTSTASQSKFST-------VLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLM 53 Query: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYATIGLLA 120 LTF TFG F MRPLGA++LGAY DR GR+ L++++ LM +GT +IAF+PSYATIG LA Sbjct: 54 LTFMTFGAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALA 113 Query: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAAIFGAVL 179 P + +G+++QGFSAG E G + +L E A P +GF+ SWQ ASQ +++++AA G +L Sbjct: 114 PLLVLIGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLL 173 Query: 180 NNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEK--TDAPLRDTFAH 237 + LTP +++ WGWR+PF G LI P IRR L E EF + T R + Sbjct: 174 STSLTPAEVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIEN 233 Query: 238 QKMRLLIGAGSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVG 296 K+ ++ G V M TVS YL ++Y PT+ L L +S GL FI P++G Sbjct: 234 WKL-VIAGMMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMG 292 Query: 297 AMSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFASMPAL 356 A+SD++GR P ++T + L I+ YP +L + F ++L+++ + L Y +M Sbjct: 293 ALSDRVGRRPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVA 352 Query: 357 LADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGNKLSVSYFVIFGAVLSLIA 416 L ++ P R G SLAY++A +FGGF I T LI++T +K + ++ AV LIA Sbjct: 353 LTEVMPADVRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIA 412 Query: 417 TL 418 TL Sbjct: 413 TL 414 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 294 bits (753), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 169/398 (42%), Positives = 242/398 (60%), Gaps = 8/398 (2%) Query: 8 PGAGTTHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFG 67 P GT RRA+I++++GN LE +D +I+G FAV I+K FFP+GD+ +L+ TF TF Sbjct: 4 PVQGTDPKTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFMTFA 63 Query: 68 VSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYATIGLLAPAGIALG 127 F +RP+GA+VLG Y+D+ GRK AL +++LM +GT + A PSY +IG+ A + G Sbjct: 64 AGFVVRPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFG 123 Query: 128 KMIQGFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAAIFGAVLNNMLTPE 186 +++QGFSAGGE GS+ A LVE+A R R F S+Q +QG +LL I G V+ + E Sbjct: 124 RLLQGFSAGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIAVVYGQE 183 Query: 187 QLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTDAPLRDTFAHQKM----RL 242 ++ WR+ F GL+I P G YIRR L E PEF+ S +K D+F R+ Sbjct: 184 RMLQDYWRLAFFVGLVIGPVGWYIRRVLPETPEFERSKKKESV---DSFLEGVKANFGRI 240 Query: 243 LIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGAMSDKI 302 LIG +I TV+ +S Y TYAV++L +P S++ +GL + PLVG ++D+I Sbjct: 241 LIGIAIMIFWTVATQVSNYFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGMLADRI 300 Query: 303 GRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFASMPALLADIFP 362 G M +AL VL YP+F L +P LLL+Q I V + +Y A P +++DIFP Sbjct: 301 GPYKPMAFGAALAGVLAYPLFKMLVATPTLPTLLLVQIAISVTLGLYAACAPLVMSDIFP 360 Query: 363 VATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGNKL 400 R TG+ +Y + V IFGG +I+T LI +G+KL Sbjct: 361 TRYRATGIGTSYALGVMIFGGLTPMIVTTLIQISGDKL 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,358,376 Number of extensions: 60389 Number of successful extensions: 375 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 320 Number of HSP's successfully gapped: 18 Length of query: 427 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 339 Effective length of database: 1,203,190 Effective search space: 407881410 Effective search space used: 407881410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)