BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0143.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp... 791 0.0 gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu... 176 2e-45 gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu... 91 2e-19 >gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbaspirillum seropedicae SmR1] Length = 384 Score = 791 bits (2043), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/384 (98%), Positives = 383/384 (99%) Query: 1 MSDQQAPGFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLL 60 MS+QQA GFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLL Sbjct: 1 MSEQQAAGFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLL 60 Query: 61 VHGKLPTAAELRAYKAKLKALRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKD 120 VHGKLPTAAELRAYKAKLK+LRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKD Sbjct: 61 VHGKLPTAAELRAYKAKLKSLRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKD 120 Query: 121 DHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPSDA 180 DHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPS+A Sbjct: 121 DHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPSEA 180 Query: 181 WEKAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEI 240 WEKAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEI Sbjct: 181 WEKAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEI 240 Query: 241 QKRYDNPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKEAGSTKMFD 300 QKRYDNPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKEAGSTKMFD Sbjct: 241 QKRYDNPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKEAGSTKMFD 300 Query: 301 IAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDN 360 IAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDN Sbjct: 301 IAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDN 360 Query: 361 KIIRPSANYVGPEDLKFVPISKRK 384 KIIRPSANYVGPEDLKFVPISKRK Sbjct: 361 KIIRPSANYVGPEDLKFVPISKRK 384 >gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 176 bits (447), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 121/395 (30%), Positives = 200/395 (50%), Gaps = 28/395 (7%) Query: 7 PGFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLLVHGKLP 66 PGF S N+++ + +L YRGY I +A +C+F E +LL+HG+LP Sbjct: 72 PGFMSTASC--------NSSITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELP 123 Query: 67 TAAELRAYKAKLKALRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKDDHNTPG 126 A + + + + + + +HPM V+ V AL + D + Sbjct: 124 NAEQKKVFVNTVTQHTMVHEQMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNAN 183 Query: 127 ARDI-ADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPSDAWE--- 182 R++ A R++A L +++ Y YS G +D S A+F+ ++ P + ++ Sbjct: 184 HREVSAIRMIAKLPTLVAMAYKYSI-GQPFVYPRNDLSYTANFMRMMFA-NPCEEYQVNE 241 Query: 183 ---KAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFE 239 +A+ LIL+A+HE NAST T R+ +G++ ++ I I L GP HGGANE A Sbjct: 242 VLVRALDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALN 301 Query: 240 IQKRYDNPDEAEADIRR-RVENKEV-VIGFGHPVYTISDPRNKVIKEVARKLSKEAG--S 295 + ++ + D+ I++ + +N V ++GFGH VY DPR K+++E ++ E G Sbjct: 302 MLEQIGSVDKIPEFIKQVKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHD 361 Query: 296 TKMFDIAERLETVMWD-----IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWA 350 +F +A LE + + +K++PN+D++S + +G+PT+MFT +F +ART GW Sbjct: 362 DPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWI 421 Query: 351 AHIIEQRID--NKIIRPSANYVGPEDLKFVPISKR 383 A E D KI RP +VG PI KR Sbjct: 422 AQWNEMISDPEQKIGRPRQLFVGSPRRDVPPIDKR 456 >gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 96/379 (25%), Positives = 156/379 (41%), Gaps = 44/379 (11%) Query: 1 MSDQQAPGFKPKKSVALSGVTAGNTAL--CTVGKTGNDLHYRGYDILDVADSCEFEEIAH 58 ++ Q+A G KPK+ VA + + G L G L YRG D L +A+ EE+A Sbjct: 51 VAAQRAHGRKPKE-VAKAALNWGAPVLESSITLIDGGRLFYRGVDALSLAEHASLEEVAA 109 Query: 59 LLVHGKLPTA--AELRAYKAKLKALRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVL 116 L TA A A L+AL + +A LP + D T + Sbjct: 110 GLWQCDRRTAFGPHGAASPAVLRALFRHYGDQRAEEVLLPLFAVASDDAST------AIW 163 Query: 117 PEKDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEK 176 + + G D+ L A L + T D+ H + Sbjct: 164 QKAVERQAQGCGDLVRILAACL------------------LRTRPDTSPIH-VQCARAWG 204 Query: 177 PSDAWEKAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEV 236 A + + +L+L A+HE NAS+FT R +A T + + + + + L G +HG Sbjct: 205 VGRAGGELIRMALVLCADHELNASSFTSRCVASTDASLRAVVIAGLAGLSGGRHGATTG- 263 Query: 237 AFEIQKRYDNPDEAEAD--IRRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKEAG 294 ++ +D + D +R R+ + + GFGH +Y DPR + +++ L + + Sbjct: 264 --RVEALWDELGTRDVDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL--LSQILPRHSP 319 Query: 295 STKMFDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHII 354 M D L P++D+ + +P LF + R+ GW AH + Sbjct: 320 WAAMIDAGITL-------TGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSIGWIAHGL 372 Query: 355 EQRIDNKIIRPSANYVGPE 373 EQR + +IRP A Y+GP+ Sbjct: 373 EQREQHHLIRPRAVYIGPQ 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,273,018 Number of extensions: 54146 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 3 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)