BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0004.0142.p
         (145 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312418|ref|YP_003776510.1| signal peptide protein [Herbasp...   224   2e-60
gi|300312282|ref|YP_003776374.1| hypothetical protein Hsero_2978...    77   4e-16

>gi|300312418|ref|YP_003776510.1| signal peptide protein
           [Herbaspirillum seropedicae SmR1]
          Length = 143

 Score =  224 bits (572), Expect = 2e-60,   Method: Compositional matrix adjust.
 Identities = 115/145 (79%), Positives = 124/145 (85%), Gaps = 2/145 (1%)

Query: 1   MIAAIVSRRLSALPLTSFVLAGAVAVALSACSSTPVPVTETKPVLPQLLQSPAATQPSWA 60
           MIA++ S R S  PL     A  +  + +  S   VPVTETKPVLPQLLQSPAATQP+WA
Sbjct: 1   MIASLHSLRSSFRPLCLAASAAVLLASCA--SREEVPVTETKPVLPQLLQSPAATQPTWA 58

Query: 61  TQLKTGAFSCEMGNKVEIRMDGRVTDGVTLVWKGSSYMMNPVSTSTGAVRLENKGEGLVW 120
           TQLKTGAFSCEMGNKVE+RMDGRVTDGVTLVWKG +YMMNPVSTSTGAVRLENKGEGLVW
Sbjct: 59  TQLKTGAFSCEMGNKVELRMDGRVTDGVTLVWKGKNYMMNPVSTSTGAVRLENKGEGLVW 118

Query: 121 IQIPSKSMLLNSKIGQQLANDCRTR 145
           IQIPSKSMLLN+KIGQQLANDCRTR
Sbjct: 119 IQIPSKSMLLNAKIGQQLANDCRTR 143


>gi|300312282|ref|YP_003776374.1| hypothetical protein Hsero_2978
           [Herbaspirillum seropedicae SmR1]
          Length = 182

 Score = 77.4 bits (189), Expect = 4e-16,   Method: Compositional matrix adjust.
 Identities = 35/77 (45%), Positives = 49/77 (63%)

Query: 68  FSCEMGNKVEIRMDGRVTDGVTLVWKGSSYMMNPVSTSTGAVRLENKGEGLVWIQIPSKS 127
           F CEMG+K+ I  +      + + W    + +  V T+TGA R EN+  GLVWI IP+K 
Sbjct: 81  FQCEMGHKLTIWKNETDDKHIAIRWDKRIHRLTRVETTTGANRFENRKMGLVWIGIPAKG 140

Query: 128 MLLNSKIGQQLANDCRT 144
           +LL+SK GQQLAN+C+ 
Sbjct: 141 LLLDSKKGQQLANECKN 157


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.127    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 391,578
Number of extensions: 13846
Number of successful extensions: 69
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 69
Number of HSP's successfully gapped: 2
Length of query: 145
Length of database: 1,619,870
Length adjustment: 77
Effective length of query: 68
Effective length of database: 1,255,275
Effective search space: 85358700
Effective search space used: 85358700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 126 (53.1 bits)