BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0141.p (628 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 1188 0.0 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 421 e-119 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 164 2e-41 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 163 3e-41 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 139 9e-34 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 1188 bits (3074), Expect = 0.0, Method: Compositional matrix adjust. Identities = 573/628 (91%), Positives = 599/628 (95%) Query: 1 MQYEDFYRRSIEQPEAYWAEEAQRIHWDKPFTRTLDYSRAPFARWFIGGTTNLCFNAVDR 60 MQYE FYR+S+EQPEA+WA+EAQRIHW +PF+RTLDYSR PFARWFI G TNLC NAVDR Sbjct: 1 MQYEQFYRQSVEQPEAFWAQEAQRIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDR 60 Query: 61 HLGERGKQAALIAISTETNTERVYDFAELHREVQAMAATMLSLGVRRGDRVLIYMPMIAE 120 HL ER +QAALIAISTETN+ERVY +AEL +EV AMAA+M +LGV+RGDRVLIYMPMIAE Sbjct: 61 HLPERAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPTLIVSADAGSRGGKVIPYKGLLDE 180 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQP LIVSADAGSRGGKVIPYKGLLDE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 181 AINLAQHKPQKVLLVDRGLAEMARTAQRDVDYAPLREQHLDQPVPVAWLESNETSYVLYT 240 AI +AQH+PQ VLLVDRGLA MARTAQRDVDYAPLR QHL Q VPV WLESN +SY+LYT Sbjct: 181 AIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILYT 240 Query: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGKPGETYFCTSDIGWVVGHSYIVYGPLIAG 300 SGTTGKPKGVQRDVGGYAVALASSMQHIFCG+PGETYFCTSDIGWVVGHSYIVYGPLIAG Sbjct: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIAG 300 Query: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPAECMERHDTSTLKALY 360 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQP ECMER+DTS+LKALY Sbjct: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDTSSLKALY 360 Query: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYR 420 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGI DKPTRLGSPGVPMPGYR Sbjct: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPMPGYR 420 Query: 421 LAILNEATGEPCAADEKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG 480 LAIL+EATGEPC D+KGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG Sbjct: 421 LAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG 480 Query: 481 IKDQDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAIAF 540 I+D+DGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVA AF Sbjct: 481 IRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF 540 Query: 541 AIPKQADAIATPERRRALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 AIPKQA IATPE+R+ALEAEIM VVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS Sbjct: 541 AIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 Query: 601 ICEGRDPGDLPTIEDPAALEQVRAVIKD 628 ICEGRDPGDLPTIEDPAALEQVRAVIKD Sbjct: 601 ICEGRDPGDLPTIEDPAALEQVRAVIKD 628 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 421 bits (1083), Expect = e-119, Method: Compositional matrix adjust. Identities = 244/624 (39%), Positives = 373/624 (59%), Gaps = 25/624 (4%) Query: 15 EAYWAEEAQ-RIHWDKPFTRTLDYSRAPFARWFIGGTTNLCFNAVDRHLGERGK--QAAL 71 E WA A+ + W KPFT+TLD S APF +WF G N+ +N +D +L E G + A+ Sbjct: 44 EGTWARLAKDNLKWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDVNL-ENGNADKTAV 102 Query: 72 IAISTETNTERVYDFAELHREVQAMAATMLSLGVRRGDRVLIYMPMIAEAVFAMLACARI 131 I S + RV + ELH++V A + SLG+++GDRV+IYMPM E V AM ACARI Sbjct: 103 IFESDDGKVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARI 161 Query: 132 GAVHSVVFGGFASNSLASRIDDAQPTLIVSADAGSRGGKVIPYKGLLDEAINLAQHKPQK 191 GA HSVVFGGF++ SL R+ DA +++AD RGGK +P K ++DEA+++ K Sbjct: 162 GATHSVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLK 221 Query: 192 -VLLVDRGLAEMARTAQRDVDYAPLREQHLDQPVPVAWLESNETSYVLYTSGTTGKPKGV 250 V++ R AE+ RD+ + + D P W+++ ++LYTSG+TGKPKGV Sbjct: 222 HVVVYKRAGAEINWVEGRDLWLSDVVANQSDTCEP-EWVDAEHPLFILYTSGSTGKPKGV 280 Query: 251 QRDVGGYAVALASSMQHIFCGKPGETYFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLP 310 Q GY + +M+ F KP + Y+CT+DIGW+ GH+YI YGPL G +++EG+P Sbjct: 281 QHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVP 340 Query: 311 IRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPAECMER-----HDTSTLKALYLAGEP 365 P+AG +W +V ++K T ++APTAIR L K A+ E+ +D S+L+ L GEP Sbjct: 341 TYPNAGRFWDMVARHKATIFYTAPTAIRSLIKA-ADADEKVHPKQYDLSSLRILGSVGEP 399 Query: 366 LDETTSSWI---SGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYRLA 422 ++ W G P++D +WQTE+G +IS G + P GS +P+PG A Sbjct: 400 INPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGAT--PQVPGSCTLPLPGITAA 457 Query: 423 ILNEATGEPCAADEKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPREVYSTFDWGI 481 I++E TG G++ ++ P P ++T++ D ERF +Y+ L ++Y D + Sbjct: 458 IVDE-TGNDIPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAV 515 Query: 482 KDQD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAIAF 540 ++++ GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D+ G+ AF Sbjct: 516 RNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAF 575 Query: 541 AIPKQADAIATPERRRALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 + K+ T + + + E+ V K+IG + +P + F LPKTRSGK++RR ++ Sbjct: 576 VVLKRPR--PTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRV 633 Query: 601 ICEGRD-PGDLPTIEDPAALEQVR 623 + +G D+ T+E+PA L+Q++ Sbjct: 634 LAKGEAITQDVSTLENPAILDQLK 657 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 159/568 (27%), Positives = 263/568 (46%), Gaps = 57/568 (10%) Query: 45 WFIGGTTNLCFNAVDRHLGERGKQAALIAISTETNTERVYDFAELHREVQAMAATMLSLG 104 W I N+ A DR G+ A + + + T YD +L A + + G Sbjct: 26 WQIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYD--QLKILSDRFANALRAAG 83 Query: 105 VRRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPTLIVSADA 164 V RGDR+ IY+ E V LA ++GA+ +F F +++A R+D++ +V+ + Sbjct: 84 VGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDAS 143 Query: 165 GSRGGKVIPYKGLLDEAINLAQHKPQKVLLVDRGLAEMARTAQRDV-DYAPLREQHLDQP 223 G E LA P L+ +++ D D LD Sbjct: 144 GM-------------EKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELD-- 188 Query: 224 VPVAWLESNETSYVLYTSGTTGKPKGV---QRDVGGYAVALASSMQHIFCGKPGETYFCT 280 PV L +++ + ++YTSGTTGK KG R + G+ + S H + G+ ++ Sbjct: 189 -PVLTL-ADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFPQEGDRFWTP 244 Query: 281 SDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVL 340 +D W+ G ++ L G+A V + DA + ++ ++++ +F PTA+++L Sbjct: 245 ADWAWIGGLLDVLLPSLYHGVAVVARR--LEKFDAAEVFGLLARHQIRNVFFPPTALKML 302 Query: 341 KKQPAECMERHDTSTLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAK 400 + A + +L+++ GE L + +W AL V + + Y QTE + ++ Sbjct: 303 RG--AATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECN---LVVSS 357 Query: 401 GISDKPTRLGSPGVPMPGYRLAILNEATGEPCAADEKGVVAIEGPLPPGCMQTVYGDDER 460 P+ GS G +PG+ + I++E G+ G +AI P P ++ Sbjct: 358 SSHCYPSVSGSMGRAVPGHVVQIVDE-QGQVLPHGTVGNIAIRAPDPVMFLR-------- 408 Query: 461 FVNTYWRS--LPREVYS-----TFDWGIKDQDGYYFILGRTDDVINVAGHRLGTREIEES 513 YWR+ RE ++ T D G D+ GY LGR DDVI AG+R+G IEE Sbjct: 409 ----YWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEEC 464 Query: 514 ISSHPNVSEVAVVGVEDKLKGQVAIAFAIPKQADAIATPERRRALEAEIMGVVDKQIGAV 573 + HP V AVVGV+D L+ +V AF + K TP+ AL+AE+ V Q+ A Sbjct: 465 LMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG---VTPD--EALKAELQQHVRAQLAAH 519 Query: 574 GRPARVFFVSGLPKTRSGKLLRRAIQSI 601 P + FV+ LP T +GK++R+ ++ + Sbjct: 520 EYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 152/561 (27%), Positives = 252/561 (44%), Gaps = 48/561 (8%) Query: 52 NLCFNAVDRHLGERGKQAALIAISTETNTERVYDFAELHREVQAMAATMLSLGVRRGDRV 111 N C DRH+ +G AL +S ++ Y FA++ +A + G++ G V Sbjct: 38 NACVECCDRHV--KGDNIALEFVSLHGEHQQ-YSFAQVRSMAARVANLLRQQGIQPGQIV 94 Query: 112 LIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPTLIVSADAGSRGGKV 171 +P E + +L R GAV+ +F F ++ R+ + +LIV+ A Sbjct: 95 AGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVAN------ 148 Query: 172 IPYKGLLDEAINLAQHKPQKVLLVDRGLAEMARTAQRDVDYAPLREQHLDQPVPVAWLES 231 + LDE N Q + R + R D+D+ + D PV Sbjct: 149 ---RDKLDEIANCPQ------VCTVRESNDPLRAG--DIDFRAAVDAQSDDFAPV-LRSG 196 Query: 232 NETSYVLYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGKPGETYFCTSDIGWVVGHSY 291 + ++ TSGTTG KGV + A + M+ P + ++ +D GW G Y Sbjct: 197 ADLMLMMSTSGTTGAAKGVPVPLSALQ-AFSVYMREAVGLLPQDKFWNMADPGWAYGLYY 255 Query: 292 IVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPAECMERH 351 + GPL G E + + + +T + APTA R++ E Sbjct: 256 AIIGPLAIGHGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAA-GEEAAAS 311 Query: 352 DTSTLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGS 411 L+A+ AGEPL+ W + AL VP+ D+Y QTE G +++ + + GS Sbjct: 312 VKGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGM-VVNNHHALRHEVVP-GS 369 Query: 412 PGVPMPGYRLAILNEATGEPCAADEKGVVAIEGPLPPGCMQTVYGDDERFVNTYWR---- 467 G MPGYR+ +L+E E A++ G +AI+ P +Y + + YW+ Sbjct: 370 AGFAMPGYRVVVLDENHQE-LGANQPGELAIDIARSP-----LY-----WFSGYWKQDTP 418 Query: 468 SLPREVYSTFDWGIKDQDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVG 527 ++ YST D + +G +GR+DDVI AG+R+G ++E ++ HP V++VAV+G Sbjct: 419 AIAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIG 478 Query: 528 VEDKLKGQVAIAFAIPKQADAIATPERRRALEAEIMGVVDKQIGAVGRPARVFFVSGLPK 587 + D + ++ AF + +D + AL ++ V +++ A P + F+ LPK Sbjct: 479 LPDPERTEIVKAFVV--LSDQFKASD---ALREQLAQHVKRRLSAHAYPRAIEFLDALPK 533 Query: 588 TRSGKLLRRAIQSICEGRDPG 608 T SGKL R ++ + R G Sbjct: 534 TPSGKLQRFVLRKMEADRRAG 554 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 139 bits (349), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 135/560 (24%), Positives = 242/560 (43%), Gaps = 87/560 (15%) Query: 71 LIAISTETNTERVYDFAELHREVQAMAATMLSLGVRRGDRVLIYMPMIAEAVFAMLACAR 130 ++A ++R+ + E++ V A+A + GV++GD++L++ + + A + Sbjct: 50 ILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFK 109 Query: 131 IGAVHSVVFGGFASNSLASRIDDAQPTLIVSADAGSRGGKVIPYKGLLDEAINLAQHKPQ 190 +G V PT ++ P EA L Q Sbjct: 110 MGVVWV-------------------PTNF----------RITP-----PEAAYLGQSSGA 135 Query: 191 KVLLVDRGLAE-----------------MARTAQRDVDYAPLREQHLDQPVPVAWLESNE 233 V+L DRG A+ +A ++DY L + A ++ ++ Sbjct: 136 CVMLYDRGFAQYVDAVKAASPALEHVVALADARAGELDYEALVREGSGAAFREAEVDYDD 195 Query: 234 TSYVLYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGKPGETYFCTSDIGWVVGHSYIV 293 + YTSGTTG PK G A + + + + PG T+ S + + H V Sbjct: 196 PLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM---PGLTHQSRSLVVAPLSHGAGV 252 Query: 294 YGPLIA--GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPAECMERH 351 + + G A++L + + W +VEKY+V +F+ PT +++L + E ++R+ Sbjct: 253 HAVINTARGAASILLSSERLVVEEA--WQLVEKYRVDNLFTVPTIVKILVED--ESVDRY 308 Query: 352 DTSTLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGI-----SDKP 406 D S+LK + AG P+ + L ++ Y E I + + + Sbjct: 309 DHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQ 368 Query: 407 TRLGSPGVPMPGYRLAILNEATGEPCAADEKGVVAIEGPLPPGCMQTVYGDDERFVNTYW 466 R+G+ G+P G ++AILN+ G+ A E G + + GP V+ Sbjct: 369 ARVGTCGMPRTGMQIAILND-DGQELAPFETGEICVRGP-------AVFMGYHNNPEANA 420 Query: 467 RSLPREVYSTFDWGIKDQDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVV 526 ++ + + T D G DQDG+ +I GR+ D+ G + REIEE++ +HP VSEVAV+ Sbjct: 421 KAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVL 480 Query: 527 GVEDKLKGQVAIAFAIPK---QADAIATPERRRALEAEIMGVVDKQIGAVGRPARVFFVS 583 GV D G+ +A + K Q D A ++ ++ +IG P R + Sbjct: 481 GVPDPKWGESGLAVIVCKAGQQVDGEA-----------LLAHLESRIGKYKWPRRFIYWD 529 Query: 584 GLPKTRSGKLLRRAIQSICE 603 +PK+ GK++++ I+++ E Sbjct: 530 SMPKSGYGKIVKKQIKALLE 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,137,225 Number of extensions: 97354 Number of successful extensions: 313 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 13 Length of query: 628 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 537 Effective length of database: 1,188,985 Effective search space: 638484945 Effective search space used: 638484945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)