BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0111.p (175 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312390|ref|YP_003776482.1| lipoprotein [Herbaspirillum ser... 326 6e-91 gi|300311082|ref|YP_003775174.1| transmembrane protein [Herbaspi... 61 7e-11 >gi|300312390|ref|YP_003776482.1| lipoprotein [Herbaspirillum seropedicae SmR1] Length = 175 Score = 326 bits (836), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 153/175 (87%), Positives = 161/175 (92%) Query: 1 MREPIFTRLRRHAGHFGALLVALAMFGCDRNGNPIEEFGLDKLQKGVSSEADVRGVMGQP 60 M +P FTRLRRHAG FGA LVALAM GCDRNG PIEEFGLDKLQKG+SSEADVR VMGQP Sbjct: 1 MTDPFFTRLRRHAGKFGAFLVALAMLGCDRNGKPIEEFGLDKLQKGISSEADVRDVMGQP 60 Query: 61 DTVWEDGGGAKTLEYPKGPEGIRTWMFRIGASGTLIDYTQVLTQEHFDTIRPGMSTEQIR 120 DTVW+DG GA+TLEYPKGP+GIRTWMF IGASGTLIDY QVLTQEHFDTIR GM+ EQIR Sbjct: 61 DTVWDDGQGARTLEYPKGPQGIRTWMFSIGASGTLIDYQQVLTQEHFDTIRAGMTAEQIR 120 Query: 121 REFGRPRSVVQFARKNEEVWDWKYHYVHEDRLFNVHFDITTKQVVRTSISEISGH 175 R+FGRPRSVVQF RKNEEVWDWKYHYVHEDRLFNVHFD+ TKQVVRTSISEISGH Sbjct: 121 RQFGRPRSVVQFGRKNEEVWDWKYHYVHEDRLFNVHFDMNTKQVVRTSISEISGH 175 >gi|300311082|ref|YP_003775174.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 177 Score = 60.8 bits (146), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 37/97 (38%), Positives = 48/97 (49%), Gaps = 2/97 (2%) Query: 49 SEADVRGVMGQPDTVWEDGGGAKTLEYPKGPEGIRTWMFRIGASGTLIDYTQVLTQEHFD 108 E V +G P V+ DG + EY GP +M RIG LI Y QV T E+F Sbjct: 44 PEDQVVARLGPPTNVYPDGN-VRVFEYGAGPFSQYQYMARIGPDHRLISYEQVWTIENFR 102 Query: 109 TIRPGMSTE-QIRREFGRPRSVVQFARKNEEVWDWKY 144 IRP T+ + R GRP V ++AR E W++ Y Sbjct: 103 AIRPKQDTKLDVLRRVGRPTEVTRYARIPFEAWNYGY 139 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 586,416 Number of extensions: 24889 Number of successful extensions: 48 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 47 Number of HSP's successfully gapped: 2 Length of query: 175 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 96 Effective length of database: 1,245,805 Effective search space: 119597280 Effective search space used: 119597280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)