BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0101.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herba... 874 0.0 gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthe... 122 4e-29 gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Her... 66 4e-12 >gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 874 bits (2258), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/461 (93%), Positives = 433/461 (93%), Gaps = 21/461 (4%) Query: 1 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI 60 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI Sbjct: 17 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI 76 Query: 61 IEGMQWLGLEHDEGPFYQMKRMDRYREVIGQMLEQGTAYYCYCTPEEVEAMRERQRAAGE 120 IEGMQWLGLEHDEGPFYQMKRMDRYREVI QMLEQGTAYYCYCTPEEVEAMRERQRAAGE Sbjct: 77 IEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGE 136 Query: 121 KPRYDGTWRPEAGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA 180 KPRYDGTWRPE GKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA Sbjct: 137 KPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA 196 Query: 181 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA 240 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA Sbjct: 197 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA 256 Query: 241 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCEWFDLDHLTK 300 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFC WFDLDHLTK Sbjct: 257 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTK 316 Query: 301 AP---------------------GFXADLVRPLLQAQGAVFEGAPDLAAVIALLKDRANT 339 AP A LVRPLL+AQGAVFEGAPDLAAVIALLKDRANT Sbjct: 317 APAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFEGAPDLAAVIALLKDRANT 376 Query: 340 VNEIAAAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATVEWSKEAIAPMIKEVLA 399 VNEIAAAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATVEW+KEAIAPMIKEVLA Sbjct: 377 VNEIAAAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATVEWTKEAIAPMIKEVLA 436 Query: 400 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVEAR 440 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAV+AR Sbjct: 437 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQAR 477 >gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 294 Score = 122 bits (307), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 93/259 (35%), Positives = 131/259 (50%), Gaps = 17/259 (6%) Query: 9 RFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAIIEGMQWLG 68 RFAPSP+G LH G AL S+ AR G ++LRIED D R+ A Q II + L Sbjct: 9 RFAPSPSGPLHAGSLVAALASYLDARVHAGRWLLRIEDIDETRTVQGAAQDIIATLAALD 68 Query: 69 LEHDEGPFYQMKRMDRY---REVIGQMLEQGTAYYCYCTPEEVEAMRERQRAAGEKPRYD 125 + D Q +R RY R+ +G + AY C C+ +E+ R + G Y Sbjct: 69 MRSDGPVLVQSQRKARYQLARDRLGAL-----AYPCGCSRKEIADSRVGTASDGAA-LYP 122 Query: 126 GTWRPEAGKTLPAIPEGRKPVVRFKNP-QDGE-VSWNDVVKGPITIS-NRELDDLVIARP 182 GT R L + R +R +P Q GE V + D GP E+ D V+ R Sbjct: 123 GTCR----HGLAPGKQARTLRLRVPDPGQAGELVQFEDRWLGPQAQHLAAEVGDFVLQRA 178 Query: 183 DGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGADG 242 DG Y VVVDD + +T ++RG D +++T RQI + LG P Y HVP+++ G Sbjct: 179 DGFWAYQLAVVVDDAEQGVTDIVRGADLLDSTARQIYLQGLLGYPTPRYLHVPLLMNETG 238 Query: 243 EKLSKRHGAVSV-MDYPAQ 260 EK SK++GA ++ ++ P Q Sbjct: 239 EKFSKQNGAQALDLNQPLQ 257 >gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 583 Score = 66.2 bits (160), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 12/149 (8%) Query: 5 QVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAIIEGM 64 QV TRF P P GYLH+G A++ ++ A + G +R +DT+ E+ E V I++ + Sbjct: 44 QVVTRFPPEPNGYLHIGHAKSICLNFGLAADYAGRCHMRFDDTNPEKEEQEYVDTILDSV 103 Query: 65 QWLGL--EHDEGP--FYQMKRMDRYREVIGQMLEQGTAYYCYCTPEEVEAMRERQRAAGE 120 +WLG EH+ +Y D ++ +G A Y + E MRE R Sbjct: 104 KWLGFSWEHEGKSHLYYASNYFDVMYACAQALIRKGLA---YVDEQNAEQMRE-NRGTLT 159 Query: 121 KPRYDGTWR----PEAGKTLPAIPEGRKP 145 +P + WR E+ + + +G+ P Sbjct: 160 EPGKNSPWRDRPAEESLRLFEEMRDGKHP 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,558,455 Number of extensions: 71319 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 3 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)