BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0091.p (302 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 573 e-165 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 273 2e-74 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 269 2e-73 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 269 2e-73 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 81 9e-17 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 573 bits (1476), Expect = e-165, Method: Compositional matrix adjust. Identities = 289/301 (96%), Positives = 292/301 (97%), Gaps = 3/301 (0%) Query: 1 MSKNAFSRSLTAAAVACTVSLATTAMTALPALAQDANTVKIGVLTDMSGPYSAMGGQGSV 60 M KNA RSLTAAAVACTVSLA TA +PA AQDANTVKIGVLTDMSGPYSAMGGQGSV Sbjct: 1 MRKNALGRSLTAAAVACTVSLAATA---IPAWAQDANTVKIGVLTDMSGPYSAMGGQGSV 57 Query: 61 VAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVAL 120 VAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVAL Sbjct: 58 VAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVAL 117 Query: 121 AVQKLIKEKGGIAMYSGPATGALTTAECATNGFHWMFDTYSQAAGAAAALTKLGNKSWYF 180 AVQKLIK+KGGIAMYSGPATGALTTAECA NGFHWMFDTYSQAAGAAAALTKLGNKSWYF Sbjct: 118 AVQKLIKDKGGIAMYSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYF 177 Query: 181 VTVDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLANGG 240 VTVDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLA+GG Sbjct: 178 VTVDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGG 237 Query: 241 TDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAF 300 TDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAF Sbjct: 238 TDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAF 297 Query: 301 S 301 S Sbjct: 298 S 298 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 273 bits (697), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 137/265 (51%), Positives = 183/265 (69%), Gaps = 2/265 (0%) Query: 37 NTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGVT 96 + +KIG +TD+SG YS + GQG A KMA+ D + G KIE+L AD+QNK DV + Sbjct: 29 DVIKIGFITDISGVYSDIDGQGGAEAIKMAIAD-MGGAINGKKIELLVADHQNKADVAAS 87 Query: 97 RAREWLDRDKVEAIADLTNSSVALAVQKLIKEKGGIAMYSGPATGALTTAECATNGFHWM 156 +AREW D+ V+ + TNS ALA K+ EK + M G + LT EC+ H+ Sbjct: 88 KAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTVHYA 147 Query: 157 FDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNA 216 +DT + A G +A+ K G KSW+F+T DYAFGHSLE D ++VVKA+GGTVVG+V+HPL A Sbjct: 148 YDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHPLGA 207 Query: 217 SDFSSFLLQAQASKAQVIGLANGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALG 276 SDFSSFLLQAQ+SKAQ+IGLAN G D +N++K A F + K +L LLVF++DVH+L Sbjct: 208 SDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGI-TKTAKLAGLLVFINDVHSLT 266 Query: 277 LEAAQGLVYTDGFYWDYDEQTRAFS 301 L+ +GL TD +YWD +E+TRAF+ Sbjct: 267 LKNTEGLYLTDSWYWDANEETRAFA 291 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 269 bits (688), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 144/296 (48%), Positives = 193/296 (65%), Gaps = 7/296 (2%) Query: 7 SRSLTAAAVACTVSLATTAMTALPALAQDAN-TVKIGVLTDMSGPYSAMGGQGSVVAAKM 65 S L A AC ++ + + ALAQ +N V+IG +TDMSGPY+ G G + A +M Sbjct: 4 STPLRRLAAACAIA----GIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRM 59 Query: 66 AVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKL 125 AV D G IE++SAD+QNK D+ TRAREW D ++ + NS+ AL++ K+ Sbjct: 60 AVAD-FGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKV 118 Query: 126 IKEKGGIAMYSGPATGALTTAECATNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDY 185 + EK + + G T LT EC H+ +DT + A G A+A+ K G KSW+F+ DY Sbjct: 119 MAEKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADY 178 Query: 186 AFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLANGGTDVVN 245 AFGHSL D S VVKA+GGTVVGS++HP +SD SSFLLQAQASKAQ++GLAN G D VN Sbjct: 179 AFGHSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVN 238 Query: 246 AIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFS 301 +IK A+ F V K +LV LL+ ++D+HALGL A+GL+ TD +YWD DE +R F+ Sbjct: 239 SIKAAKEFGV-TKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFA 293 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 269 bits (687), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 142/296 (47%), Positives = 188/296 (63%), Gaps = 6/296 (2%) Query: 6 FSRSLTAAAVACTVSLATTAMTALPALAQDANTVKIGVLTDMSGPYSAMGGQGSVVAAKM 65 F R +A AC A LP+ +T+KIG +TDMSG Y+ GQG V A +M Sbjct: 4 FIRRAALSATAC----ALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRM 59 Query: 66 AVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKL 125 A+ D G K+E++ AD+QNK D+ +AREW DRD V+ + NS+ +LA+ K+ Sbjct: 60 AIADA-GGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKV 118 Query: 126 IKEKGGIAMYSGPATGALTTAECATNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDY 185 EK + + G T T EC + +DT + A G AA+T+ G KSWYF+T DY Sbjct: 119 AGEKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADY 178 Query: 186 AFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLANGGTDVVN 245 AFG SLEKD S+VVK+NGG VVG+ R PL SDFSSFLLQAQ+SKAQ++GLA G DV+N Sbjct: 179 AFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVIN 238 Query: 246 AIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFS 301 AIK A F V K +L LL+F++D H+LGL QG+ TDG+YWD ++QTRA++ Sbjct: 239 AIKAANEFGV-NKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWA 293 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 81.3 bits (199), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 69/259 (26%), Positives = 115/259 (44%), Gaps = 10/259 (3%) Query: 36 ANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGV 95 A ++ G MSGP++A G+ + + K+A++ K G ++ D + KP V Sbjct: 28 AESIAFGCPVPMSGPFAA-NGKFADLGMKLALQKYGK--VLGKSTSYVNLDTEGKPATAV 84 Query: 96 TRAREWLDR-DKVEAIADLTNSSVALAVQKLIKEKGGIAMYSGPATGALTTAECATNGFH 154 + +E +++ + A L+ ++A+ + +K G + +T ++C F Sbjct: 85 RKMQESIEKGTRFFAGGILSGEALAMGKEA---DKAGAVFITTAGADEITGSDCNRATFR 141 Query: 155 WMFDTYSQAAGAAAAL-TKLGN-KSWYFVTVDYAFGHSLEKDASDVVKANGGTVVGSVRH 212 W T+ L K+ N K WY +T Y FG L A ++ K G VG+ H Sbjct: 142 WSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYH 201 Query: 213 PLNASDFSSFLLQAQASKAQVIGLANGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDV 272 L +FS +L A A+K V+ L N G+ + ++QA SF + L+A L Sbjct: 202 SLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQF 261 Query: 273 HALGLEAAQGLVYTDGFYW 291 LG + G VY YW Sbjct: 262 ETLGADICDG-VYFGAQYW 279 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 843,547 Number of extensions: 31323 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 13 Length of query: 302 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 217 Effective length of database: 1,217,395 Effective search space: 264174715 Effective search space used: 264174715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)