BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0090.p (580 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 1014 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 462 e-131 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 456 e-129 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 220 3e-58 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 199 5e-52 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 1014 bits (2623), Expect = 0.0, Method: Compositional matrix adjust. Identities = 489/491 (99%), Positives = 490/491 (99%) Query: 81 QANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTW 140 QANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTW Sbjct: 3 QANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTW 62 Query: 141 RNTSLAQRMRIMLKFQQLLRENIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITS 200 RNTSLAQRMRIMLKFQQLLRENIAPLAE+ITREHGKTLPDAEGEVMRGLEVVEHACSITS Sbjct: 63 RNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITS 122 Query: 201 LQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQ 260 LQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQ Sbjct: 123 LQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQ 182 Query: 261 DPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRA 320 DPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRA Sbjct: 183 DPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRA 242 Query: 321 SEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTREWI 380 SEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTREWI Sbjct: 243 SEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTREWI 302 Query: 381 PEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGS 440 PEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGS Sbjct: 303 PEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGS 362 Query: 441 ENGNFVGPTVFTGVKPEMDIYAQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSS 500 ENGNFVGPTVFTGVKPEMDIY QEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSS Sbjct: 363 ENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSS 422 Query: 501 GYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVT 560 GYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVT Sbjct: 423 GYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVT 482 Query: 561 ARWYAPDEEAG 571 ARWYAPDEEAG Sbjct: 483 ARWYAPDEEAG 493 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 462 bits (1188), Expect = e-131, Method: Compositional matrix adjust. Identities = 222/475 (46%), Positives = 310/475 (65%), Gaps = 1/475 (0%) Query: 90 YLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQRM 149 Y+ G++V + S + DV NPA VA+V T +EVD AV F W NT R Sbjct: 7 YIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRA 66 Query: 150 RIMLKFQQLLRENIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 209 R++ +F L+++ A +I REHGKT DA+GEV RG+E+VE A I + GE + Sbjct: 67 RVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 Query: 210 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 269 +A G+D +++ Q LGV AGIT FNFP M+P +MFPIA+ACGN FVLKPSE+DP++SL + Sbjct: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 Query: 270 ELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQC 329 +L +AGLP GV NVV G + + DHP +KA+SF+GST + +IY R S GKR Q Sbjct: 187 DLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQA 246 Query: 330 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQT-REWIPEIVERSK 388 + GAKNH +V+PDA A++ L+GAA+G+AG+RCMA SV + VG E + + ER++ Sbjct: 247 LGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAALAERAR 306 Query: 389 AMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENGNFVGP 448 +K+ G A++GP+V+ AAKER+E LI GVE+GA L++DGR +V G E G FVG Sbjct: 307 KLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVGG 366 Query: 449 TVFTGVKPEMDIYAQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYAARKFQ 508 T+ V PEM +Y +EIFGP +C++ + A+ INA+ GNG +++T G AR+F Sbjct: 367 TLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREFV 426 Query: 509 NEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVTARW 563 +I+VG VG+NVP+PVP+A+ SF G + S GD G + V F+T+ K V RW Sbjct: 427 RQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQRW 481 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 456 bits (1172), Expect = e-129, Method: Compositional matrix adjust. Identities = 238/488 (48%), Positives = 327/488 (67%), Gaps = 5/488 (1%) Query: 83 NIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRN 142 ++P++ ++NG+ +S S +DV NPAT A+V A+ +EV+ AVA A W Sbjct: 2 SLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAE 61 Query: 143 TSLAQRMRIMLKFQQLLRENIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQ 202 T+ +R R++ KF++L+ +N LA+ IT EHGK DA+GEV RGLE+VE A I L Sbjct: 62 TAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLL 121 Query: 203 LGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDP 262 + +N+ GG+D + L QPLGV AGIT FNFPVM+P +M PIA+A GN+FVLKPSE+DP Sbjct: 122 KTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 Query: 263 TSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASE 322 + SL L EL +AGLP GV NVV G + + +HP+++AVSF+GST + +IYR+A+E Sbjct: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 Query: 323 -AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 378 AG RAQ + GAKNH +V+PDA DQA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 Query: 379 WIPE-IVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKV 437 + E ++ R KA+KV G + A++GPLV+ A K +VE I SGV+ GAKLL+DGR KV Sbjct: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 Query: 438 AGSENGNFVGPTVFTGVKPEMDIYAQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIF 497 G E G F+G T+F V EM IY +EIFGP +C+V + A+ INA+ GNG S+F Sbjct: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 Query: 498 TSSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTK 557 T+ G A +F I VG VGINVPIPVP+A+ SF G + S GD G++ + F+T+ K Sbjct: 422 TADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYK 481 Query: 558 TVTARWYA 565 ++ RW A Sbjct: 482 SIMQRWSA 489 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 220 bits (560), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 150/477 (31%), Positives = 231/477 (48%), Gaps = 9/477 (1%) Query: 86 NVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSL 145 +V L++NGE S S DV+NPAT+EV+ ++ A + ++DRA+ A + F+TWRN S Sbjct: 4 DVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSA 63 Query: 146 AQRMRIMLKFQQLLRENIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGE 205 +R +IM + LLRE +A ++T E GK L +A+ E + + ++ G Sbjct: 64 FERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGR 123 Query: 206 LAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSS 265 L G + +P+G A T +NFP+ A+A G + ++K E+ P S Sbjct: 124 LVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 266 LFLVELANQAGLPPGVLNVVHGGP-DVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAG 324 L+ AG+P GV+N+V+G P +++ + HP IK +SF GST VG + A + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 325 KRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMA-NSVVVLVGQTREWIPEI 383 KRA +G +V DA D A L A F AGQ C++ +V G ++ + Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 384 VERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENG 443 V ++ +KVG G + +GP+ +K +E LI V QGA+L G+ G Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRI----GNKG 359 Query: 444 NFVGPTVFTGVKPEMDIYAQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYA 503 F PTV T + + +E FGP I +TLDE I N P G F S + Sbjct: 360 YFFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHT 419 Query: 504 ARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVT 560 ++ G + IN + + + F G + S G G G +A+ + TK VT Sbjct: 420 KHALAARVESGMLTIN-HLGLALPELPFGGIKDSGYGSEG--GTEAIEAYINTKLVT 473 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 199 bits (506), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 135/458 (29%), Positives = 222/458 (48%), Gaps = 7/458 (1%) Query: 90 YLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQRM 149 ++NG+ + E V NPA E++ VP E RA+ A + W+ + +R Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 150 RIMLKFQQLLRENIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 209 I+ ++ +L+ N LA ++T E GK L +A GE+ +E G+ + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 210 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 269 + + + + +GV A IT +NFP + A+A G VLKP+E P S+L L Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 270 ELANQAGLPPGVLNVVHGGPD-VANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQ 328 LA +AG+P GV +VV G P + + +P ++ +SF GST VG + ++++ + K+ Sbjct: 195 VLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLS 254 Query: 329 CMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCM-ANSVVVLVGQTREWIPEIVERS 387 +G IV DA D A+ + + + AGQ C+ AN + V G + ++VE Sbjct: 255 LELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAV 314 Query: 388 KAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENGNFVG 447 K KVG GT+ GPL+++ A ++VE+ +A V +GA++LL G+ + S F Sbjct: 315 KKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHS----FFE 370 Query: 448 PTVFTGVKPEMDIYAQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYAARKF 507 PTV V P M + +E FGP + T +E +A N G + ++ + Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 508 QNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPN 545 ++ G VGIN + + F G + S LG G + Sbjct: 431 AEGLESGMVGINTGL-ISNEVAPFGGVKQSGLGREGSH 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,757,300 Number of extensions: 73311 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 22 Length of query: 580 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 490 Effective length of database: 1,193,720 Effective search space: 584922800 Effective search space used: 584922800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)