BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0033.p (696 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis pro... 656 0.0 gi|300312325|ref|YP_003776417.1| chemotaxis signal transduction ... 195 1e-50 gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I M... 96 1e-20 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 83 7e-17 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 76 7e-15 >gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 656 bits (1692), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/394 (90%), Positives = 373/394 (94%) Query: 1 MKNWTIKQRILGSFGLILLLMALMAGISAVNLGHIEKGATALRDDSTPGLYYSTQINAAW 60 MKNWTIKQRILGSF LILLLMALMAGISAVNLG IE GATALR DSTPGLYYST INAAW Sbjct: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 Query: 61 YENLLMTQQIIQIDANDAERQADLALLRRNAERLDRLQEDYDKTIFSDADRKLFEQFKET 120 YENLLM+QQI QID N+AERQADLALLRRN ERLDRLQEDYDKTIFSD+DRKLF+QFKET Sbjct: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET 120 Query: 121 RQKYRAVVSEVLKMDFSQRAPIDKIVNEQIRPQWRTGYQLTQRMVETNKGIADDAAQRIG 180 RQKYRA++SEVLKMDFSQRAPIDK+ NEQIRPQWR GYQLTQRMV+TNK IADDAA RI Sbjct: 121 RQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRIN 180 Query: 181 DAVNSAQFTLMSALMAALLVALVCGFFLMRAINEPLQKLMGLLDIMRSGDFSRRVELDRK 240 DAV SAQF ++S+LMAALL ALVCGFFLMRAINEPLQKLMG+LD MRSGDFSRRV LDR Sbjct: 181 DAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRRVALDRN 240 Query: 241 DEFGVLGNGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 DEFG LG+GFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG Sbjct: 241 DEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 Query: 301 ATSREIHATSRDLVRTMNEVSVVSEQTSALAGSGQAGLTRMGDTMQNVMDAAGSVSAKLA 360 ATSREIH+TSRDLVRTMNEVSVVSEQTSALAG+GQAGLTRM DTMQNVMDAAGSVSAKLA Sbjct: 301 ATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQNVMDAAGSVSAKLA 360 Query: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA Sbjct: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 >gi|300312325|ref|YP_003776417.1| chemotaxis signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 158 Score = 195 bits (495), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 103/151 (68%), Positives = 114/151 (75%) Query: 541 LMSQSLHLLFSLGEERYALPTAEVAMVLPLSPCKSLPGAPAWVAGLLPHGSGHVPVIDLS 600 +++ L LLFSLG +RYALP AEVA+VLPL+PCK LPGAPAWVAGLLP+ HVPVIDLS Sbjct: 3 VVTDPLFLLFSLGADRYALPVAEVAVVLPLTPCKQLPGAPAWVAGLLPYEGRHVPVIDLS 62 Query: 601 MLALGHPAPARVSTRLVLVHYRHAQALPVLLGLILPQATDTLRLDPAHFTPAAVRNEGAP 660 LG PA RVSTRLVLVH R P LLGLIL QATDTLR F A V ++ P Sbjct: 63 QRTLGRPAAQRVSTRLVLVHDRSPAGEPALLGLILEQATDTLRCAEQQFVRATVAHDETP 122 Query: 661 YLGPVMPYQGGLLQRVEVGDLLDPATRALLY 691 YLGPVMPYQGGLLQRV+V LLD ATRA+LY Sbjct: 123 YLGPVMPYQGGLLQRVQVSALLDAATRAVLY 153 >gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I MCP-I serine chemoreceptor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 556 Score = 95.9 bits (237), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 105/417 (25%), Positives = 190/417 (45%), Gaps = 54/417 (12%) Query: 1 MKNWTIKQRIL--GSFGLILLLMALMAGISAVNLGHIEKGATALRDDSTPGLYYSTQINA 58 M ++IK R++ SF I+LL + + G+ V+L + +L +D Y QI + Sbjct: 1 MNKFSIKIRLILAISFLTIMLLASGIVGL--VSLHSTNEKMRSLYEDRLVAYAYLEQIES 58 Query: 59 AWYENLLMTQQIIQIDA------NDAERQADLALLRRNAERLDRLQEDYDKTIFSDADRK 112 II +D+ N+ +R+ DL L N + ++ + Y T + + Sbjct: 59 TLESARSGIYGIITLDSSEGWDPNEIDRRLDL--LVNNLKTVEPVIRSYQATYLTPEEST 116 Query: 113 LFEQFKETRQKY--RAVVSEVLKMDFSQRAPIDKIVNEQIRPQWRTGYQ----LTQRMVE 166 L E+ + RQ Y V+ V + D I + +R QW +Q L + + Sbjct: 117 LLERLLKQRQHYADEGVMPAVESLRKRSFQGADDIYSGAMRKQWDAVHQTLSDLKELQIN 176 Query: 167 TNKGIADDAAQRIGDAVNSAQFTLMS--ALMAALLVALVCGFFLMRAINEPLQKLMGLLD 224 + + + QR Q LM+ A+ A L+VA++ + L+RAI++PL + +G+ Sbjct: 177 VGRELYEHEQQRY------RQLILMALLAMGAGLIVAVLEAWLLVRAISQPLTQAVGIAQ 230 Query: 225 IMRSGDFSRRVELDRKDEFGVLGNGFDRMTDELTALVSQAQKSSARVATSVTEI------ 278 + GD SR++E+ +DE G L MT L +V+ ++ + + + EI Sbjct: 231 AVAGGDLSRQIEVRSQDETGQLMQALKDMTQRLYQIVAVVRQGTETITMASKEITSGNLD 290 Query: 279 -AATARQQQATAAETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGSGQAG 337 +A QQ + ETA+ ++ +T ++ +A + + Q + LA S Sbjct: 291 LSARTEQQAGSLEETASAMAQLTSTVKQ-NADN-------------ASQANQLAASASEV 336 Query: 338 LTRMGDTMQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 R G+ ++ V+D S+ N+ + I ++ I +A QTN+L+LNAA+EA Sbjct: 337 AVRGGEVVRQVVDTMTSI-------NQSSTRIVDIIAVIDGIAFQTNILALNAAVEA 386 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 83.2 bits (204), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 101/408 (24%), Positives = 181/408 (44%), Gaps = 46/408 (11%) Query: 3 NWTIKQRILGSFGLILLLMALMAGISAVNLGHIEKGATALRDDSTPGLYYSTQINAAWYE 62 N I +R+ F LIL ++AGI L H+ A A R+ L + ++ A WY Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHV---AQATREMMAEPLK-TERLMADWYT 61 Query: 63 NLLM-TQQIIQIDANDAERQADLAL-------LRRNAERLDRLQEDYDKTIFSDADRKLF 114 NL+ ++ I I + +D AL + +++ Q+ + + + ++ LF Sbjct: 62 NLVAGIRRTIAI-----AKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALF 116 Query: 115 EQFKETRQKYRAVVSEVLKMDFSQRAPIDKIVNEQIRPQWRTGYQLTQR-MVETNKGIAD 173 ++ E R+ Y + E+ K + + +Q+ T YQ T R +V+ + D Sbjct: 117 QKIGELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMD 176 Query: 174 DAAQRIGDAVNSAQFTLMSALMAALLVALVCGFFLMRAINEPLQKLMGLLDIMRSGDFSR 233 D A+ I ++ ++ LL+ +V +L I +PL + + + + GD S Sbjct: 177 DTAREIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSS 236 Query: 234 RVELDRKDEFGVLGNGFDRMTDELTALVSQAQKSSARVATSVTEIAA-------TARQQQ 286 + + +DE G L M L +VS + + ++T+ +EIAA QQ Sbjct: 237 EIAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQA 296 Query: 287 ATAAETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGSGQAGLTRMGDTMQ 346 ++ AETA+ E+ +T R+ +R + N S V+EQ G + Sbjct: 297 SSLAETASAMEELISTVRQNADNARQASQLANSASAVAEQG--------------GGVVS 342 Query: 347 NVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 V+D G++ N + I +++ I +A QTN+L+LNAA+EA Sbjct: 343 QVVDTMGAI-------NASSRKIVDIISVIDGIAFQTNILALNAAVEA 383 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 76.3 bits (186), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 106/415 (25%), Positives = 185/415 (44%), Gaps = 56/415 (13%) Query: 1 MKNWTIKQRILGSFGLILLLMALMAGISAVNLGHIEKGATALRDDSTPGLYYS----TQI 56 + N TI+ R+ FGL+++LM MA LG + + D P + Q+ Sbjct: 3 LSNVTIRVRLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVDQL 62 Query: 57 N--AAWYENLLMTQQIIQIDANDAER--QADLALLRRNAERLDRLQEDYDK----TIFSD 108 N A N+L+ ++ QI ++ ER AD A+ R + +LD+L D D T Sbjct: 63 NLIARSVRNILLLKREPQIK-SEYERISGADRAI-REDLAQLDKLLTDGDSRAALTTLVT 120 Query: 109 ADRKLFEQFKETRQKYRAVVSEVLKMDFSQRAPIDKIVNEQIRPQWRTGYQLTQRMVETN 168 A FE+ R K A+V + + ID +++E +RP ++ Sbjct: 121 AQNAYFEK----RDKVLAMVQQ-----GGKEGAIDMLIDE-VRPVQSATMNAADALIRQQ 170 Query: 169 KGIADDAAQRIGDAVNSAQFTLMSALMAALLVALVCGFFLMRAINEPLQKLMGLLDIMRS 228 + A + A+ L+ A L A V + + R+I PL + + + + + + Sbjct: 171 HEMMKTAGDEVESNYAQARTLLLLLAAAGLAAAAVIAWMITRSITAPLDRAVRVAETVAA 230 Query: 229 GDFSRRVELDRKDEFGVLGNGFDRMTDELTALVSQAQKSSARVATSVTEIA-------AT 281 GD + ++ +DE +L +M D L +VSQ + S+ +AT+ +EIA + Sbjct: 231 GDLTSAIDAHGRDETSMLLRALKKMNDRLLDIVSQVRVSTDTIATASSEIARGNLDLSSR 290 Query: 282 ARQQQATAAETAATTTEIGATSRE--IHATSRDLVRTMNEVSVVSEQTSALAGSGQAGLT 339 +Q A+ ETA++ ++ AT ++ HA Q S LAGS Sbjct: 291 TEEQAASLEETASSMEQLTATVKQNASHAG----------------QASQLAGSASDIAR 334 Query: 340 RMGDTMQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 + G+ + +V++ G++ A + I ++ I +A QTN+L+LNAA+EA Sbjct: 335 QGGEVVGHVVETMGAIDA-------SSKKIVDIIGVIDGIAFQTNILALNAAVEA 382 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,129,904 Number of extensions: 94984 Number of successful extensions: 560 Number of sequences better than 1.0e-08: 62 Number of HSP's gapped: 496 Number of HSP's successfully gapped: 62 Length of query: 696 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 604 Effective length of database: 1,184,250 Effective search space: 715287000 Effective search space used: 715287000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)