BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0032.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 404 e-114 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 96 5e-21 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 89 4e-19 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 82 6e-17 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 67 3e-12 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 404 bits (1038), Expect = e-114, Method: Compositional matrix adjust. Identities = 236/331 (71%), Positives = 259/331 (78%), Gaps = 22/331 (6%) Query: 1 MKSLPLIADKLKAVMGLDMATVGSSLIERVVRERMAALGIADDGHYLARLQGSAAELQAL 60 MKSL IADKLKA MGLDMATVG+SLIERVVRERMA L + DD YL+ L SAAELQAL Sbjct: 1 MKSLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQAL 60 Query: 61 IELAVVPETWFFRDREAIFAVARLARERLLSQPDVPVRILSLPCSTGEEPYSVAMALLEA 120 IELAVVPETWFFRDREAI AVARLA ++L +QP +RILSLPCSTGEEPYS+AMALL+A Sbjct: 61 IELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA 120 Query: 121 GVMPSQFVIDAYDIRTRSLEIAAAGVYGRNSFRGQALGFRDRHFMTVEQG-WQLEEAIRR 179 G+ P QFVIDAYDIRTRSLEIAAAG+YGRNSFRGQ L FRDRHF +EQG W+L E IR Sbjct: 121 GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHF-DLEQGQWRLHERIRS 179 Query: 180 QVRFAPGNLLAPDLLRQAAPYDFIFCRNVLIYFEREVQRQVVSLLESLMKPDAVIFVGPA 239 QVRFAPGNLLAPD LR + PYDFIFCRNVLIYFER+VQRQVV LL S+MKPDA+IFVGPA Sbjct: 180 QVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPA 239 Query: 240 EGGLMLSPRLESAGIALAFGFRKR----VAVEKPAWRETVLAA------AQPLLAAPVPA 289 EGG++L P +ESAGIALAFGFRKR V PAW V AA +P A PA Sbjct: 240 EGGILLGPTMESAGIALAFGFRKRVAAAVPPLPPAWASLVQAAPPAPTPVRPDAAHRAPA 299 Query: 290 RRAXGIGSSAA--AVGCACSASRLFLRPAFP 318 RR I +SAA AVG A +A RPA P Sbjct: 300 RR---IVASAALGAVGTATAA-----RPASP 322 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 95.5 bits (236), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 66/235 (28%), Positives = 106/235 (45%), Gaps = 12/235 (5%) Query: 16 GLDMATVGSSLIERVVRERMAALGIADDGHY--LARLQGSAAELQALIELAVVPETWFFR 73 G+ +++ + ER+ A G HY L R + ELQ L L V ET+F R Sbjct: 23 GISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLLTNLMTVNETYFLR 82 Query: 74 DREAIFAVAR-----LARERLLSQPDVPVRILSLPCSTGEEPYSVAMALLEA--GVMPSQ 126 + + + R R + P+RI +PCS+GEE Y++AM L+E G+ Sbjct: 83 EEYQFQCLVNSMLPEITRNR---RSTAPIRIWCIPCSSGEEAYTLAMYLMERWPGIDQWD 139 Query: 127 FVIDAYDIRTRSLEIAAAGVYGRNSFRGQALGFRDRHFMTVEQGWQLEEAIRRQVRFAPG 186 I + DI T L A G Y S + + ++F+ +Q+ +A+R V F Sbjct: 140 VEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQICDALRGAVEFTRV 199 Query: 187 NLLAPDLLRQAAPYDFIFCRNVLIYFEREVQRQVVSLLESLMKPDAVIFVGPAEG 241 NL + +D IFCRN+LIYF+ +++ + + I +G +E Sbjct: 200 NLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNGFICLGHSES 254 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 73/245 (29%), Positives = 111/245 (45%), Gaps = 13/245 (5%) Query: 16 GLDMATVGSSLIERVVRERMAALGIADDGHYLARLQ--GSAAELQALIELAVVPETWFFR 73 G+ +A ++ + R+ A GI YL L+ + E +A T FFR Sbjct: 35 GIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFR 94 Query: 74 DREAIFAVARLARERLLSQPDVPVRILSLPCSTGEEPYSVAMALLEA-GVMPSQFVIDAY 132 + +A L P I STGEEPY++AM EA G + I A Sbjct: 95 EAHHFPILADF-----LKTLKEPATIWCSAASTGEEPYTIAMTACEAYGSLTPPVQIVAT 149 Query: 133 DIRTRSLEIAAAGVYGRNSFRGQALGFRDRHFMT---VEQGW-QLEEAIRRQVRFAPGNL 188 DI T L + GVY + + F+ +G+ ++ +R+ + F NL Sbjct: 150 DIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVRVRPELRKLITFKQLNL 209 Query: 189 LAPDLLRQAAPYDFIFCRNVLIYFEREVQRQVVSLLESLMKPDAVIFVGPAEGGLMLSPR 248 LA D + +D IFCRNV+IYF++ Q +++S LMKP A++F G +E L +S Sbjct: 210 LA-DGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSENFLYVSDA 268 Query: 249 LESAG 253 L+ G Sbjct: 269 LKLKG 273 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 82.0 bits (201), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 78/259 (30%), Positives = 123/259 (47%), Gaps = 26/259 (10%) Query: 11 LKAVMGLDMATVGSSLIERVVRERMAALGIADDGHYLARL--QGSAAELQALIELAVVPE 68 K +GL + +L++ + R+ LG+ G Y A + + A E Q I+L E Sbjct: 25 FKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQERQRAIDLITTNE 84 Query: 69 TWFFRDREAIFAVARLARERLLSQPDVPVRILSLPCSTGEEPYSVAMALLEAGVMPSQFV 128 T+FFR+ V +L QP + RI S +TGEE Y++AM LL+ + + Sbjct: 85 TYFFREPGHFDFVVGQWLPQLAQQPQL--RIWSAASATGEEAYTLAM-LLDHHRPGASWS 141 Query: 129 IDAYDIRTRSLEIAAAGVY----GRNSFRG-------QALGFRDRHFMTVEQGWQLEEAI 177 + A DI TR L A +Y G+N R +G + HF+ ++ + Sbjct: 142 VFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYEGHFL-------VQADL 194 Query: 178 RRQVRFAPGNLLAPDLLRQAAPYDFIFCRNVLIYFEREVQRQVVSLLESLMKPDAVIFVG 237 R +V+FA NL D YD IF RNV+IYF+ E + QV+ + + ++P + VG Sbjct: 195 RAKVQFAQRNLTRLDE-HDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVVG 253 Query: 238 PAEG--GLMLSPRLESAGI 254 +E G+ L ++S I Sbjct: 254 HSESLHGMDLGLDMQSPSI 272 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 64/217 (29%), Positives = 101/217 (46%), Gaps = 27/217 (12%) Query: 31 VRERMAALGIADDGHYLARLQG----SAAELQALIELAVVPETWFFRDREAIFAVARLAR 86 ++ R+A I D L++LQ AA L+E V + FRD A+ Sbjct: 41 LKRRLATAMIRFDCQTLSQLQDRVLHDAAVFPLLMEYLTVQVSEMFRDPSYFLALREKVV 100 Query: 87 ERLLSQPDVPVRILSLPCSTGEEPYSVAMALLEAGVMPSQFVIDAYDIRTRSLEIAAAGV 146 L + P ++I CS+GEE YS+A+ L E G++ +I A DI +L+ A AGV Sbjct: 101 PLLRTYP--SLKIWVAGCSSGEEVYSLAILLREEGLLERS-IIYATDINQSALKKAEAGV 157 Query: 147 YGRNSFRGQALGFRDRH-----------FMTVEQG-WQLEEAIRRQVRFAPGNLLAPDLL 194 Y + GF + H + T G ++++R+ V F+ +L + Sbjct: 158 YDLE----RIAGFTNNHQKSGARTSLSDYYTAAYGAVVFDKSLRKNVVFSDHSLATDSVF 213 Query: 195 RQAAPYDFIFCRNVLIYFEREVQRQVVSLL-ESLMKP 230 A + CRNVLIYF R++Q + + L ESL++ Sbjct: 214 ---AEMHLVSCRNVLIYFNRQLQERALGLFRESLVRK 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 880,965 Number of extensions: 35699 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 5 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)