BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0024.p (451 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 798 0.0 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 586 e-168 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 314 7e-87 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 305 4e-84 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 287 9e-79 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 798 bits (2062), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/450 (92%), Positives = 426/450 (94%), Gaps = 4/450 (0%) Query: 1 VNPSQSSPATNQVLTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLATDLGIGAAA 60 +NPSQSS LTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHL+TDLGIGAAA Sbjct: 1 MNPSQSSTPAGLALTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAA 60 Query: 61 FGLGAGLFFVGYAIFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVHSETSFYFMRF 120 FGLGAGLFFVGYA+FEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFV SETSFYFMRF Sbjct: 61 FGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRF 120 Query: 121 LLGVTEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH 180 LLGV EAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH Sbjct: 121 LLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH 180 Query: 181 GWQWMFIIEGGASMLFCVVVWKVLDSTPRDAKWLSPEQREALATVIAAEQEQRAANRPAH 240 GWQWMFIIEGGASM+FCVVVW VLDSTPR+AKWLS EQREALA VIAAEQEQRAANRPAH Sbjct: 181 GWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAH 240 Query: 241 VSALTLLRDPQIVLFCLIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI 300 VSAL+LLRDPQIVLFC IYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI Sbjct: 241 VSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI 300 Query: 301 AAMYVFAVLAARYKKPQFWVATTLVISAIGMAVSASAGPVIGFVAVCFAGIGFKAASSLF 360 AAMY+FAVLAARYKKPQFWVATTLVISA GMA+SA AGPVIGFVAVCFAGIGFKAASSLF Sbjct: 301 AAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLF 360 Query: 361 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLYALSGASLVAA 420 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGL+ALSGASL+AA Sbjct: 361 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAA 420 Query: 421 VLVFLARTRPSVQDGGGSPEGSTEPVAKHA 450 VLVFLARTRP+ DGGG E + KHA Sbjct: 421 VLVFLARTRPAAPDGGGPAEAAV----KHA 446 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 586 bits (1511), Expect = e-168, Method: Compositional matrix adjust. Identities = 300/418 (71%), Positives = 347/418 (83%) Query: 13 VLTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLATDLGIGAAAFGLGAGLFFVGY 72 VL AV+KIK R+LPLFV MFI+NYIDRVNIGFVRSHLATD+GIG AA+GLGAGLFFVGY Sbjct: 25 VLQQAVAKIKQRVLPLFVIMFIVNYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGY 84 Query: 73 AIFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVHSETSFYFMRFLLGVTEAGFFPG 132 A+FEVPSN+LLQR+GA+AWLTRIM TWGL A MAFV+SET+FY +RFLLG EAGFFPG Sbjct: 85 ALFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPG 144 Query: 133 VVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGA 192 V+YYFTQWLP ERGRAMA+FLSGSA+ASILSGPI+G LL I+GGGL GWQWMFIIEG A Sbjct: 145 VIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQIDGGGLQGWQWMFIIEGMA 204 Query: 193 SMLFCVVVWKVLDSTPRDAKWLSPEQREALATVIAAEQEQRAANRPAHVSALTLLRDPQI 252 S+L C VW LDS P DAKWL+ ++R A+ I EQ +R ++PA VS LLRDPQI Sbjct: 205 SVLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMKHQPAQVSPRQLLRDPQI 264 Query: 253 VLFCLIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYVFAVLAAR 312 ++FC IYF+I LTIYGATFWLP+II+KMG F+DFQVGLFNSIPW+++I AMY FA LAAR Sbjct: 265 LIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAAR 324 Query: 313 YKKPQFWVATTLVISAIGMAVSASAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARI 372 +K Q W AT LVI+A GM S V FV++CFA +GFKAASSLFWP+PQAYLDARI Sbjct: 325 FKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARI 384 Query: 373 AAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLYALSGASLVAAVLVFLARTRP 430 AA V+ALINS+GNLGGF AP FG+LEQ+TGSIQGGL L+ SLVAA +VFLAR+ P Sbjct: 385 AAGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGP 442 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 314 bits (805), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 166/410 (40%), Positives = 244/410 (59%), Gaps = 9/410 (2%) Query: 19 SKIKWRILPLFVAMFIINYIDRVNIGFVRSHLATDLGIGAAAFGLGAGLFFVGYAIFEVP 78 SK+ WR+LPL ++ +Y+DRVN+GF + + DL +GLGAG+FF+GY +FEVP Sbjct: 22 SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVP 81 Query: 79 SNMLLQRYGARAWLTRIMFTWGLVAACMAFVHSETSFYFMRFLLGVTEAGFFPGVVYYFT 138 SNM+L + GAR W+ RIM TWG+++ MA+V + FY MRFLLGV EAGFFPGV+ Y T Sbjct: 82 SNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLT 141 Query: 139 QWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGG--GLHGWQWMFIIEGGASMLF 196 W P RG+ A+F++G A++ ++ GP++G ++ G G GWQWMFI+E S++ Sbjct: 142 YWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLIL 201 Query: 197 CVVVWKVLDSTPRDAKWLSPEQREALATVIAAEQEQRAANRPAHVSALTLLRDPQIVLFC 256 V+V + RDA WLS E++ L I+ E+ Q+ H+S + + ++ L Sbjct: 202 GVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK-----EHLSLGAMFANGRVWLGA 256 Query: 257 LIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYVFAVLAARYKKP 316 LIYF + +YG +FWLPTIIK G + VGL +IP+ A AAM + A ++ Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 317 QFWVATTLVISAIGMAVSASAG--PVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAA 374 ++ VA I IG+ +S G + ++ A +G LFW +P A+L AA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 375 AVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLYALSGASLVAAVLVF 424 A +ALINSLGNL GF +P G+L+ T S G+Y L+ + ++ +L Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTL 426 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 305 bits (782), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 160/420 (38%), Positives = 252/420 (60%), Gaps = 10/420 (2%) Query: 19 SKIKWRILPLFVAMFIINYIDRVNIGFVRSHLATDLGIGAAAFGLGAGLFFVGYAIFEVP 78 K+ WRI+P + ++I Y+DRVN+GF + ++ DLG FGLGAGLFF+GY +FEVP Sbjct: 23 KKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVP 82 Query: 79 SNMLLQRYGARAWLTRIMFTWGLVAACMAFVHSETSFYFMRFLLGVTEAGFFPGVVYYFT 138 SN+L+ + GAR W+ RIM TWG+++A FV + T FY +RFLLG+ EAGF+PG++ Y T Sbjct: 83 SNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLT 142 Query: 139 QWLPNHERGRAMAVFLSGSAIASILSGPITGALL--LIEGGGLHGWQWMFIIEGGASMLF 196 W P+H R + +AVF+SG +A IL P++G ++ + GGL GWQWMF+IE ++L Sbjct: 143 YWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLI 202 Query: 197 CVVVWKVLDSTPRDAKWLSPEQREALATVIAAEQEQRAANRPAHVSALTLLRDPQIVLFC 256 V LD+ + AKWL+ E++ +L A+ + A + + +++D ++ L C Sbjct: 203 GVATVLYLDNDVKSAKWLNDEEKASL----QADIDGDAKGKESKHGFGAIVKDARVWLMC 258 Query: 257 LIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYVFAVLAARYKKP 316 LIYF+ + YG T W+PT++K G + ++GL ++IP+ AI AM + A R ++ Sbjct: 259 LIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRER 318 Query: 317 QFWVATTLVISAIGMAVSA--SAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAA 374 ++ + ++ +G +A + + ++ A G + LFW +P A+L AA Sbjct: 319 RWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAA 378 Query: 375 AVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLYALSGASLVAAVLVFLARTRPSVQD 434 +A INS+GNL GF +P GYL+ T + G+Y L+ LV + LA T+P + + Sbjct: 379 VGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLA-VMLVVGSIATLA-TKPGMVN 436 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 287 bits (735), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 176/442 (39%), Positives = 249/442 (56%), Gaps = 22/442 (4%) Query: 2 NPSQSSPATNQVLTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLATDLGIGAAAF 61 N SQ S L K+ W+ILP + ++I Y+DRVN+GF + + +DLG+ AA+ Sbjct: 3 NASQGSAVQFDGL---YKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAY 59 Query: 62 GLGAGLFFVGYAIFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAF---------VHSE 112 GLGAG+FF+GY IFEVPSN++L R GA+ W+ RIM TWG++++ MAF V +E Sbjct: 60 GLGAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNE 119 Query: 113 TSFYFMRFLLGVTEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALL 172 T FY +RFLLG +EAGFFPGV+ Y T W P R MAV L G+ + IL GPI+GAL+ Sbjct: 120 TVFYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALM 179 Query: 173 LIEGGG--LHGWQWMFIIEGGASMLFCVVVWKVLDSTPRDAKWLSPEQREALATVIAAEQ 230 G G L GWQWMF+IE + L +V+ VL + AKWL+PE++ LA +AAE Sbjct: 180 QFMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAEN 239 Query: 231 EQRAANRPAHVSALTLLRDPQIVLFCLIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGL 290 +++ N P +L P + L+Y + + YG FW PTII+ G + +G+ Sbjct: 240 KEK-VNVPLK----SLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGM 294 Query: 291 FNSIPWIVAIAAMYVFAVLAARYKKPQFWVATTLVISAIGMAVSA--SAGPVIGFVAVCF 348 ++IP+I+ AM A + K + + +++ + + + I + Sbjct: 295 ISAIPYILGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAI 354 Query: 349 AGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGG 408 A G + +LFW P AY AAA +A+INS+GNLGGF P G L GS + G Sbjct: 355 AVGGSLTSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYG 414 Query: 409 LYALSGASLVAAVLVFL-ARTR 429 L L GA +A VL+ L R R Sbjct: 415 LMMLGGALFLAGVLIALGGRKR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,359,194 Number of extensions: 53583 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 341 Number of HSP's successfully gapped: 21 Length of query: 451 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 363 Effective length of database: 1,203,190 Effective search space: 436757970 Effective search space used: 436757970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)