BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0004.0023.p
         (307 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspi...   598   e-172
gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protei...    60   2e-10

>gi|300310491|ref|YP_003774583.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 307

 Score =  598 bits (1541), Expect = e-172,   Method: Compositional matrix adjust.
 Identities = 294/307 (95%), Positives = 303/307 (98%)

Query: 1   METLYHLIEQYGLILVFVNVLVEQLGAPVPAYPTLIITGALSATGRYSPALLLLLAVSAA 60
           METLYHLIEQYGL++VFVNVLVEQLGAPVPAYPTLI+TGALSA GRYSPA+LLL+AV AA
Sbjct: 1   METLYHLIEQYGLLIVFVNVLVEQLGAPVPAYPTLILTGALSAAGRYSPAVLLLVAVCAA 60

Query: 61  LIADYCWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120
           L+ADY WYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI
Sbjct: 61  LLADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120

Query: 121 ASALSGTMGTRRISFLLWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLAQLGHYGMLMIA 180
           ASALSGTMGTRR SFLLWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLA+LGHYGMLMIA
Sbjct: 121 ASALSGTMGTRRRSFLLWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLAELGHYGMLMIA 180

Query: 181 VALAAFVANKWWQRRRFRKSLEMARISVDELNTMLEEGNAPTIIDVRSPMSQQDGRIPGA 240
           VALAAFVANKWWQRRRFRKSLEMARISVDELNTMLEEGNAPTIIDVRSP+SQQDGRIPGA
Sbjct: 181 VALAAFVANKWWQRRRFRKSLEMARISVDELNTMLEEGNAPTIIDVRSPLSQQDGRIPGA 240

Query: 241 VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAAMVARQLMRRGYTRVRPLTGGIDAWRA 300
           VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAA+VARQLMRRGYTRVRPLTGGIDAWRA
Sbjct: 241 VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAALVARQLMRRGYTRVRPLTGGIDAWRA 300

Query: 301 AGYAIDV 307
           AGYAIDV
Sbjct: 301 AGYAIDV 307


>gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 220

 Score = 60.5 bits (145), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 57/208 (27%), Positives = 99/208 (47%), Gaps = 17/208 (8%)

Query: 7   LIEQYGLIL---VFVNVLVEQLGAPVPAYP---TLIITGALSATGRYSPALLLLLAVSAA 60
           LIEQYG ++   +F+ V  E     +P  P    L I G   ATG     LL+ L V AA
Sbjct: 20  LIEQYGALIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTFCATGAMDIWLLMTLLVVAA 79

Query: 61  LIADYCWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120
           +  +   Y  G   G+++ +   R  L+P++ +R+T + Y + G +++++A+F P   + 
Sbjct: 80  VAGNTLNYWIGGWVGQRVYTHNYR-WLNPEA-LRKTHAFYEKHGGKTIVIARFTPIVRTF 137

Query: 121 ASALSGTMGTRRISFLLWDAAGSALWAGLGIFLGSLFSS--AVDDLLNVLAQLGHYGMLM 178
           A  ++G        F L++  G+ LW    + LG LF +   V   LN L  +G     +
Sbjct: 138 APFIAGVSAMSFARFQLFNVLGAFLWVVGLVVLGYLFGNIPVVQQNLNTLVLIG-----I 192

Query: 179 IAVALAAFVANKW--WQRRRFRKSLEMA 204
           +A      +   W  W++ R ++ +  A
Sbjct: 193 VAAVFPIVLGGLWQIWRKLRGKRKVSNA 220


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.138    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 896,842
Number of extensions: 34188
Number of successful extensions: 229
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 230
Number of HSP's successfully gapped: 3
Length of query: 307
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 222
Effective length of database: 1,217,395
Effective search space: 270261690
Effective search space used: 270261690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)